Addgene

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pSB3C5-proC-B0032-E0051

Plasmid

#107242

Purpose

Expresses lacZa-GFP fusion from constitutive proC promoter

Depositor

Robert Sauer, Joseph Davis

Article

Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

Insert

ProC-RBS(B0032)-Gemini (E0051)

Use

Tags

Expression

Mutation

Promoter

proC

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSB3C5-proB-B0032-E0051

Plasmid

#107243

Purpose

Expresses lacZa-GFP fusion from constitutive proB promoter

Depositor

Robert Sauer, Joseph Davis

Article

Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

Insert

ProB-RBS(B0032)-Gemini (E0051)

Use

Tags

Expression

Mutation

Promoter

proB

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCMB-PLAr

Plasmid

#61201

Purpose

Fluorescent reporter for plastids MtRubisco1sp-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRubisco1sp-mCherry

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRO288

Plasmid

#233827

Purpose

MoClo-YTK; type4A; Activation domains; VP48

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

VP48

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO129

Plasmid

#233805

Purpose

MoClo-YTK; type3; rtTA systems; rtTA3

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

rtTA3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO232

Plasmid

#233808

Purpose

MoClo-YTK; type2A; ABA systems; phlfO7

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

phlfO7

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO175

Plasmid

#233810

Purpose

MoClo-YTK; type3B; ABA systems; ABI

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

ABI

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO177

Plasmid

#233811

Purpose

MoClo-YTK; type3B; ABA systems; PYL1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

PYL1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO286

Plasmid

#233812

Purpose

MoClo-YTK; type2A; Auxin systems; amtRO7

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

amtRO7

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN022

Plasmid

#233814

Purpose

MoClo-YTK; type3B; Auxin systems; AIDΔ34

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

AIDΔ34

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN079

Plasmid

#233819

Purpose

MoClo-YTK; type3B; NS3 systems; NS3a

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NS3a

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN043

Plasmid

#233821

Purpose

MoClo-YTK; type3B; NS3 systems; DNCR2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

DNCR2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO117

Plasmid

#233823

Purpose

MoClo-YTK; type3; Reporter; mNeonGreen

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Tgl3-4V5-APEX2-URA

Plasmid

#207059

Purpose

Expressing Tgl3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Tgl3

Use

Tags

4xV5-APEX2

Expression

Yeast

Mutation

Promoter

pTGL3

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Pex3-4V5-APEX2-URA

Plasmid

#207057

Purpose

Expressing Pex3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

PEX3

Use

Tags

4xV5-APEX2

Expression

Yeast

Mutation

Promoter

pPEX3

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-RfA

Plasmid

#186370

Purpose

Destination vector for the expression of a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-Kan

Plasmid

#188928

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-Kan

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-URA

Plasmid

#188927

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-URA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-NAT

Plasmid

#188926

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-NAT

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-Phleomycin

Plasmid

#188925

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-Phleomycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge