We narrowed to 16,276 results for: nes
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Plasmid#20424DepositorPublicationInsertAMHR2 (AMHR2 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationC473G mutation when compared to Genbank reference…Available sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)
Plasmid#171089PurposeCRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.DepositorInsertsDecoy sgRNA
dCas9
Chloramphenicol acetyltransferase
UseCRISPR; Toxoplasma gondii expressionTags3X FLAG and Nuclear Localization SignalMutationD10A, H840APromoterTUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pATP416-pluxO5-pphlO6-crtYBI
Plasmid#165977PurposeExpresses carotenoid biosynthesis gene in response to homoserine lactone and 2,4-diacetylphloroglucinol in yeast expressing PhlTA and LuxTADepositorInsertsXanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)
UseSynthetic BiologyExpressionYeastMutationC66A, C1359T and C1281A, G1698APromoterSynthetic promoter (pluxO5), Synthetic promoter (…Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
R701-X52-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169)
Plasmid#159560PurposeE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169)DepositorAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X09-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D
Plasmid#159541PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) G12D (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG12DAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDSAY
Plasmid#62291Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP markerDepositorTypeEmpty backboneExpressionInsectAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDSAR
Plasmid#62292Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed RFP markerDepositorTypeEmpty backboneExpressionInsectAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pISce1-memGFP
Plasmid#32673PurposeTransgenesis with SceI meganuclease, membrane-localized EGFP reporterDepositorTypeEmpty backboneUseZebrafish transgenesisTagsEGFPAvailable sinceNov. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLY70
Plasmid#130948PurposeA CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).DepositorInsertsdxcas9 (3.7)
tetR
rhaS
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9 for dCas9; Sy…PromoterPcon, PpspA-LEA3B3, PrhaB, and PtetAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Multiplex-CRISPR-miR-200FAM-T2A-GFP
Plasmid#179856Purposeto delete miR200 family genes including miR-141, miR-200c, miR-429 and miR-200bDepositorInsertUseCRISPR and LentiviralExpressionMammalianAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VHC
Plasmid#112618Purposeexpression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsTagsVenusExpressionMammalianPromoterCAGAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVHC_PGKneoLox2DTA.2
Plasmid#112622PurposeH2BVenus_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology armsDepositorInsertsUseCre/Lox and Mouse TargetingTagsVenusPromoterNo PromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTopaz-pBAD
Plasmid#54841PurposeLocalization: Protein Expression Vector , Excitation: 514, Emission: 527DepositorTypeEmpty backboneTags6xHis and mTopazExpressionBacterialAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAW-SpyCsn2
Plasmid#113251PurposeE. coli expression vector for S. pyogenes Csn2-MBP-HisDepositorInsertS. pyogenes Csn2
TagsMBP-6xHisExpressionBacterialPromoterT7Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAW-SpyCas1
Plasmid#113248PurposeE. coli expression vector for His-MBP-S. pyogenes Cas1DepositorInsertS. pyogenes Cas1
Tags6xHis-MBPExpressionBacterialPromoterT7Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAW-SpyCas2
Plasmid#113249PurposeE. coli expression vector for His-MBP-S. pyogenes Cas2DepositorInsertS. pyogenes Cas2
Tags6xHis-MBPExpressionBacterialPromoterT7Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSECRETS-A
Plasmid#196986PurposeFor SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterpltetO1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mpCHECK2
Plasmid#167576PurposeThe mpCHECK2 plasmid has the ability to evaluate the effect of polyadenylation signal variants on expression of disease-associated genes.DepositorTypeEmpty backboneUseLuciferaseAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHERD20T-V3TnAcrVIA1
Plasmid#250984PurposepHERD20T vector providing the transposon donor with acrVIA1DepositorInsertTransposon donor V3 encoding acrVIA1
ExpressionBacterialAvailable sinceMarch 31, 2026AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEC2483 (pSpy1C)
Plasmid#220248Purposereplicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expressionDepositorTypeEmpty backboneExpressionBacterialAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only