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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,359 results for: 11

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Showing: 6841 - 6860 of 8359 results

  7. pNAS1b split mCherry 14-3-3β ctrl

    Plasmid
    #112031
    Purpose
    Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)
    Depositor
    Jesse Rinehart
    Publication
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
    Tags
    Split mCherry
    Expression
    Bacterial
    Available since
    July 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112031-plasmid-map-sequence-id-216275

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  8. myc-POMP

    Plasmid
    #86764
    Purpose
    N-terminal myc-tagged protein expression in mammalian cells
    Depositor
    Do-Hyung Kim
    Publication
    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human POMP (POMP Human)
    Tags
    myc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    86764-plasmid-map-sequence-id-170534

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  9. pcDNA3.1-nAChr-Alpha6

    Plasmid
    #48812
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Publication
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    48812-plasmid-map-sequence-id-363332

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  10. Negative Control Binder

    Plasmid
    #179140
    Purpose
    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9) and an additionional A70Q mutation greatly reduces affinity for SsrA peptide
    Depositor
    Klaus Hahn
    Publication
    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SspB
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9. Addi…
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179140-plasmid-map-sequence-id-353410

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  11. pVenusCfC

    Plasmid
    #128567
    Purpose
    Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.
    Depositor
    Oliver Vugrek
    Publication
    Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus
    Expression
    Mammalian
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    128567-plasmid-map-sequence-id-321691

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  12. pVenusCfN

    Plasmid
    #128566
    Purpose
    Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.
    Depositor
    Oliver Vugrek
    Publication
    Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus
    Expression
    Mammalian
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    128566-plasmid-map-sequence-id-513794

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  20. pANY6

    Plasmid
    #112198
    Purpose
    This plasmid is used for efficient cloning of DNA, efficient expression of protein in E. coli, and ELP-based protein purification.
    Depositor
    Yingfeng An
    Publication
    Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61
    Type
    Empty backbone
    Tags
    ELP-tag
    Expression
    Bacterial
    Available since
    Aug. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    112198-plasmid-map-sequence-id-218116

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Showing: 6841 - 6860 of 8359 results