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pFB-MeCP2.9Ch (pc2385)

Plasmid

#211720

Purpose

Expresses MeCP2 (aa163-309) mCherry tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MeCP2 aa163-309

Tags

mCherry

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MeCP2G.9 (pc1574)

Plasmid

#211719

Purpose

Expresses MeCP2 (aa163-309) GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MeCP2 aa163-309

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MeCP2G.8 (pc1573)

Plasmid

#211718

Purpose

Expresses MeCP2 (aa310-492) GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MeCP2 aa310-492

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB MBD2a-2-strep (pc1794)

Plasmid

#211715

Purpose

Expresses fl. MBD2 2xStrep tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2

Tags

2xStrep

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB MBD2a-C-GFP (pc1565)

Plasmid

#211712

Purpose

Expresses fl. MBD2 GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMBD2.1R (pc2813)

Plasmid

#211575

Purpose

Expresses MBD2 (aa1-152) RFP tagged in mammalian cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.1 aa1-152

Tags

RFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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LV-sgRNA-mCherry-Puro sgPIDD

Plasmid

#211528

Purpose

Deletes PIDD

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgPIDD

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 hygro sgUCK1

Plasmid

#211524

Purpose

Deletes UCK1

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgUCK1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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Hy_pRnrS eGFP SV40

Plasmid

#195062

Purpose

Expresses eGFP mRNA from the Drosophila RnrS promoter

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

eGFP

Expression

Insect

Promoter

RnrS

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Hy_pAna eGFP SV40

Plasmid

#195063

Purpose

Expresses eGFP mRNA from the Drosophila Ana promoter

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

eGFP

Expression

Insect

Promoter

Ana

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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hPRL1_1-169

Plasmid

#209792

Purpose

Bacterial expression of human PRL-1 without farnesylation motif

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

Insert

PRL-1 (PTP4A1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-169 only

Promoter

T7

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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hPRL1_1-169_C104D

Plasmid

#209793

Purpose

Bacterial expression of human PRL-1 C104D mutant without farnesylation motif

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

Insert

PRL-1 (PTP4A1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-169 only; C101D

Promoter

T7

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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hPRL3_1-169_C104D

Plasmid

#209797

Purpose

Bacterial expression of human PRL-3 C104D mutant without farnesylation motif

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

Insert

PRL-3 (PTP4A3 Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-169 only; C101D

Promoter

T7

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3/HF-CDC50B

Plasmid

#209224

Purpose

Mammalian expression of CDC50B

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

CDC50B (TMEM30B Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.13_GagZip-MCP_Zip-P2A-eGFP

Plasmid

#205528

Purpose

Express HIV GagZip-MCP_Zip-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

GagZip-MCP_Zip

Expression

Mammalian

Mutation

Nucleocapsid replaced with leucine zipper

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.7_Gag-dZF2-MCP-P2A-eGFP

Plasmid

#205523

Purpose

Express HIV Gag-delta-ZF2-MCP-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Gag delta ZF2-MCP

Expression

Mammalian

Mutation

ZF2 deleted

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.14_GagZip-dp1dp6-MCP-P2A-eGFP

Plasmid

#205529

Purpose

Express HIV GagZip-dp1dp6-MCP-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

GagZip-dp1dp6-MCP

Expression

Mammalian

Mutation

Nucleocapsid replaced with leucine zipper, p1 and…

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.99_PB-EPN1-MCP_I-T2A-eGFP-PGK-Blast

Plasmid

#205544

Purpose

Express EPN1-MCP-I-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN1-MCP-I

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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FB395

Plasmid

#203631

Purpose

TU for the expression of luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

R1:G1aG2b.1:mPAF:Luc:TtrpC

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FB396

Plasmid

#203632

Purpose

TU for the expression of luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

R1:G1ab.1:mPAF:Luc:TtrpC

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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