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pTK_Snai2_enh_13_Citrine

Plasmid

#130574

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Snai2_enh_13

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-Q

Plasmid

#64980

Purpose

Expresses the E108Q mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E108Q

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pMYs-IRES-mitoRFP

Plasmid

#121996

Purpose

Expression of mitoRFP in mammalian cells

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

IRES-mitoRFP

Use

Retroviral

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAcGP67A human LRP6 E1E2 (20–629)

Plasmid

#132627

Purpose

expresses human LRP6 E1E2 (20–629) in Hi5 insect cell

Depositor

Wenqing Xu

Article

Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

Insert

LRP6 (20–629)

Expression

Insect

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMZ2007

Plasmid

#204399

Purpose

A 3OC6-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

Inserts

quorum sensing regulator LuxR
GFP

Use

Synthetic Biology

Promoter

PftsH and Synthetic promoter PLux0002

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGGF002

Plasmid

#48843

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

p35S::BastaR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site in 35S promoter re…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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p70 delta2-46 delta CT104

Plasmid

#1898

Depositor

Joseph Avruch

Article

Weng et al Mol Cell Biol 1995 May;15(5):2333-40.

Insert

p70 alpha1 S6 kinase (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Mutation

delta2-46, deltaCT104 (deletion of aa 2-46 and am…

Available Since

March 17, 2006

Availability

Academic Institutions and Nonprofits only
pICSL70002

Plasmid

#50333

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Biotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH87633

Plasmid

#50271

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, nos, (Agrobacterium tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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p3PA-Puro-iTk

Plasmid

#109067

Purpose

inserts three polyA sites to end transcription of a targetted gene and a puromycin cassette for selection

Depositor

Eric Lander

Article

Engreitz et al Nature. 2016 Nov 17;539(7629):452-455. d

Type

Empty backbone

Expression

Mammalian

Promoter

EFS

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAcGP67A human LRP6 E3E4 (630–1244)

Plasmid

#132628

Purpose

expresses human LRP6 E3E4 (630–1244) in Hi5 insect cell

Depositor

Wenqing Xu

Article

Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

Insert

LRP6 (630–1244)

Expression

Insect

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGP67A human LRP6 E1E4 (20–1244)

Plasmid

#132629

Purpose

expresses human LRP6 E1E4 (20–1244) in Hi5 insect cell

Depositor

Wenqing Xu

Article

Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

Insert

LRP6 (20–1244)

Expression

Insect

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Sox9_enh_345_Citrine

Plasmid

#130592

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox9_enh_345

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Sox10_enh_87_Cerulean

Plasmid

#130586

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_87

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Sox10_enh_85_Citrine

Plasmid

#130585

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_85

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLNCX2-lifeact-mAPPLE

Plasmid

#89820

Purpose

mAPPLE labeled fluorescent actin

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

lifeact-mAPPLE

Use

Retroviral

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pGGF008

Plasmid

#48848

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pNOS::BastaR::tNOS

Use

Golden gate compatible cloning vector

Mutation

chi sequence in BastaR removed by silent substitu…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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vector-dendra2 PCDNA

Plasmid

#121134

Purpose

mammalian cell expression

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

empty cassete for contol of miR expression

Expression

Bacterial

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL70005

Plasmid

#50335

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

bialaphos resistance cassette (Nos promoter (A. tumefaciens) + phosphinothricin acetyl transferase (S. hygroscopicus) + Nos term

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pILGFP4I

Plasmid

#104504

Purpose

Used to evaluate the expression output of Saccharomyces kudriavzevii GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SkGAL1 promoter-yEGFP

Expression

Yeast

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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