We narrowed to 19,562 results for: Tec

Showing: 6921 - 6940 of 19562 results

Results per page:

Sort results by:

LLP727_L2_pV01_0I_TMV-rLUC_TCTP-fLUC

Plasmid

#192370

Purpose

To control for variation introduced to the gene circuits, Rluc expression not affected by the introduction of terminators or recombination target sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ

Plasmid

#192375

Purpose

To test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-35Ster-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP729_L2_pV01_0I_FRT-OCS-rLUC_TCTP-fLUC

Plasmid

#192372

Purpose

To test for the repressive potential of the OCS with the FRT sites around it. Then used as a control to represent the off state of many cicuits.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

Plasmid

#192374

Purpose

To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCStrunc-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ

Plasmid

#192385

Purpose

To test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ

Plasmid

#192386

Purpose

To test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ

Plasmid

#192404

Purpose

On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ

Plasmid

#192401

Purpose

Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-Rluc-B3RT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZ

Plasmid

#192400

Purpose

On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-Rluc-B3RT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ

Plasmid

#192402

Purpose

On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ

Plasmid

#192405

Purpose

Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP713_L2pV3_NOT_FRT_Rluc_1IF_lacZ

Plasmid

#192403

Purpose

Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ

Plasmid

#192381

Purpose

To test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ

Plasmid

#192379

Purpose

To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP734_L2pV1_1I_OCS-rLUC_F-NOS-FlpO

Plasmid

#192377

Purpose

To test if the NOS promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP735_L2pV1_1I_OCS-rLUC_F-TCTP-FlpO

Plasmid

#192378

Purpose

To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
EK0544 CMV-TO-mCherry-SG(s70587-dblstop)SG-EGFP (FLP-IN)

Plasmid

#191176

Purpose

Mammalian expression of EK0209 with a second stop codon.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

CMV-TO:mCherry:s70587:EGFP (double-stop)

Expression

Mammalian

Mutation

additional stop codon

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
EK0546 SFFV-mCherry-SGc(GAPDH.s1-dblstop)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191178

Purpose

Mammalian expression of EK0493 with second stop codon.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:GAPDH.s1:EGFP (double-stop)

Expression

Mammalian

Mutation

additional stop codon

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
EK0344 SFFV-mCherry-SG(Bdnf.s1)SG-Cre (FLP-IN)

Plasmid

#191158

Purpose

Expression of a sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, Cre output; 90 bp long

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s1:Cre

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NSK025 CMV-TO-EGFP:c.637A>G(N213D; CCA>CCG)-stop-t70587 (PB)

Plasmid

#191166

Purpose

Mammalian expression of a EGFP mutant N213D derived from EK0379.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

EGFP(N213D)

Expression

Mammalian

Mutation

c.637A>G(N213D; CCA>CCG)

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge