We narrowed to 27,557 results for: pET
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Plasmid#154188PurposeTo express LanCL2-C321A/C367A in bacterial cellsDepositorInsertLanCL2
TagsHexahistidineExpressionBacterialMutationCys321->Ala321; Cys367->Ala367Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR
Plasmid#169499PurposeTier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)DepositorInsertPRPBSA-driven YPet-P2aA-PuroR
ExpressionMammalianPromoterPRBSAAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1047-Tier1-PhCMV-YPet-p2A-PuroR
Plasmid#169498PurposeTier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).DepositorInsertPCMV-driven YPet-P2aA-PuroR
ExpressionMammalianPromoterPhCMVAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His10-BTLAicd (AA190-289)-TwinStrep
Plasmid#180805PurposeExpression of 10*His tagged human BTLA intracellular domain (AA190-289)-TwinStrepDepositorAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
CEA-N domain residues 1-132 (CEACAM5)-pET30b
Plasmid#174693PurposePlasmid expressing CEA-N domainDepositorInsertCEA-N Domain
TagsTEV Cleavage Site and hexahistidine (HIS) tagExpressionBacterialMutationnonePromoterT7 PromoterAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-mVenus-dspB(E184Q W330Y)-6xHis
Plasmid#176577PurposePlasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsHexahistidine tag and mVenusExpressionBacterialMutationE184Q W330YPromoterT7Available SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin-FLN-ELP-His-ybbr
Plasmid#168039PurposeExpresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertAnticalin
Tags3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-FLN-ELP-His-ybbr
Plasmid#168047PurposeExpresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
Tags3xELP linker, 6x Histag, ddFLN4, and ybbr tagExpressionBacterialPromoterT7 promoterAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)
Plasmid#91818PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutationsDepositorInsertSPT6 (SPT6 Budding Yeast)
Tags6xHisExpressionBacterialMutationchanged lysine 1355 and lysine 1435 to alanines; …PromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-YPET-3XNLS-optoNES-WPRE
Plasmid#159947Purposeoptical regulation of nuclear exportDepositorInsertYPET-3XNLS-optoNES
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES-WPRE
Plasmid#159942Purposeoptical regulation of nuclear exportDepositorInsertNLS-YPET-optoNES
UseAAVExpressionMammalianMutationsyntheticPromoterhSyn1Available SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.F434L-WPRE
Plasmid#159975Purposeoptical regulation of nuclear export with shorter relaxation timeDepositorInsertNLS-YPET-optoNES.F434L
UseAAVExpressionMammalianMutationF434LPromoterhSyn1Available SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE
Plasmid#159973Purposeoptical regulation of nuclear export with extended relaxation timeDepositorInsertNLS-YPET-optoNES.V416I
UseAAVExpressionMammalianMutationV416IPromoterhSyn1Available SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.I427V-WPRE
Plasmid#159974Purposeoptical regulation of nuclear export with shorter relaxation timeDepositorInsertNLS-YPET-optoNES.I427V
UseAAVExpressionMammalianMutationI427VPromoterhSyn1Available SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-TBP(38Q)-IDR-mCherry
Plasmid#145283PurposeBacterial Expression of 6xHis TBP(38Q) IDR mCherry Fusion ProteinDepositorInsertTBP(38Q)-IDR (TBP Human)
ExpressionBacterialAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS
Plasmid#153443PurposeE. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.DepositorInsertRc.XDocB
Tags6xHis and ybbr tagExpressionBacterialMutationSerine 199 was mutated to amber codon (TAG)PromoterT7 promoterAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q79N Q109N (TAC-->TAT)
Plasmid#105738PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein Q79N Q109N (TAC-->TAT) (SNCA Human)
ExpressionBacterialMutationQ79N/Q109NPromoterT7Available SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 K321D
Plasmid#89288PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
Tags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Lys-321 to AspPromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 R325D
Plasmid#89289PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
Tags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Arg-325 to AspPromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 E322A
Plasmid#87748PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
Tags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Glu-322 to AlaPromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only