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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,947 results for: emb

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Showing: 6941 - 6960 of 11947 results

  1. pChlr-4NYQ

    Plasmid
    #52157
    Purpose
    Encodes module 4 with amino acids 12N-13Y-16Q for recognition of nt 5U
    Depositor
    Huimin Zhao
    Publication
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 13H to 13Y in Module 4
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. pChlr-5NQ

    Plasmid
    #52161
    Purpose
    Encodes module 5 with amino acids 12N-16Q for recognition of nt 4U
    Depositor
    Huimin Zhao
    Publication
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12C to 12N in Module 5
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pChlr-6CQ

    Plasmid
    #52163
    Purpose
    Encodes module 6 with amino acids 12C-16Q for recognition of nt 3A
    Depositor
    Huimin Zhao
    Publication
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C in Module 6
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pEMS1408

    Plasmid
    #29205
    Purpose
    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested
    Depositor
    Elizabeth Simpson
    Publication
    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple201 (SLC6A5 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available since
    Nov. 15, 2011
    Availability
    Academic Institutions and Nonprofits only
    29205-plasmid-map-sequence-id-13620

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  14. pAAV hSyn INTRON mEmerald-ER WPRE

    Plasmid
    #236230
    Purpose
    AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum
    Depositor
    Jennifer Lippincott-Schwartz
    Publication
    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do
    Insert
    mEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)
    Use
    AAV
    Tags
    mEmerald
    Promoter
    human Synapsin 1
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    236230-plasmid-map-sequence-id-471247

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  16. mCherry-Dyskerin

    Plasmid
    #207577
    Purpose
    N-terminal mCherry-tag using SalI/EcoRI
    Depositor
    Jens Schmidt
    Publication
    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    generated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    LTR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207577-plasmid-map-sequence-id-429490

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  17. Mito-mEosEM-E

    Plasmid
    #226335
    Purpose
    Photoswitchable fluorescent protein for correlative light-electron microscopy; mitochondria-targeted mEosEM-E for mammalian expression
    Depositor
    Pingyong Xu
    Publication
    Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    COX8A (COX8A Human)
    Tags
    mEosEM-E
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    226335-plasmid-map-sequence-id-456241

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  18. pcDNA3.1-Tras heavy chain-GSG-SpyTag003

    Plasmid
    #216292
    Purpose
    Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab
    Depositor
    Mark Howarth
    Publication
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    Tras heavy chain-GSG-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216292-plasmid-map-sequence-id-433349

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  19. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII

    Plasmid
    #217897
    Purpose
    Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tag
    Depositor
    Brenda Schulman
    Publication
    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    Tags
    2xSTII
    Expression
    Insect
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    217897-plasmid-map-sequence-id-432185

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  20. pCAGGS-3xFLAG-bIV spectrin (repeats 13-15)

    Plasmid
    #213728
    Purpose
    Mammalian cell expression vector for overexpression of bIV spectrin (repeats 13-15) in NIH/3T3 cells.
    Depositor
    Chaolin Zhang
    Publication
    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bIV spectrin (repeats 13-15) (Sptbn4 Mouse)
    Expression
    Mammalian
    Available since
    March 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    213728-plasmid-map-sequence-id-422465

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Showing: 6941 - 6960 of 11947 results