We narrowed to 9,236 results for: mel

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beta-catenin (S33A, S37A, T41A, S45A)-pcw107

Plasmid

#64612

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Ctnnb1 (Ctnnb1 Mouse)

Use

Lentiviral

Mutation

S33A, S37A, T41A, S45A

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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SmoM2 (W535L)-pcw107

Plasmid

#64627

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

SMO (SMO Human)

Use

Lentiviral

Mutation

W535L

Promoter

PGK

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only
IKKalpha (S176E,S180E)-pcw107-V5

Plasmid

#64608

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

CHUK (CHUK Human)

Use

Lentiviral

Tags

V5

Mutation

S176E, S180E

Promoter

PGK

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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beta-catenin (S33Y)-pcw107-V5

Plasmid

#64616

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral

Tags

V5

Mutation

S33Y

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-AlkB

Plasmid

#228218

Purpose

Express wild type AlkB protein in E. coli BL21(DE3)

Depositor

Junchao Shi

Article

Shi et al Nat Cell Biol. 2021 Apr;23(4):424-436. d

Insert

Alpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)

Tags

His tag

Expression

Bacterial

Mutation

codon optimized

Promoter

T7

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mNeon-PuroR [M1G]

Plasmid

#171800

Purpose

Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mNeonGreen-T2A-PuroR

Use

Gene tagging

Mutation

PuroR: Changed Methionin 1 to Glycine.

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-GATA4

Plasmid

#101417

Purpose

Donor Vector containing GATA4 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

GATA4 (GATA4 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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NES-EGFP-PH-BTKx2

Plasmid

#183652

Purpose

PtdIns(3,4,5)P3 biosensor

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

BTK (BTK Frog, Human)

Tags

X. laevis map2k1.L(32-44)-EGFP

Expression

Mammalian

Mutation

Amino acids 2-174 and 2-170

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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AR-V7-pcw107-V5

Plasmid

#64636

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

AR (transcript variant 1, splice isoform) (AR Human)

Use

Lentiviral

Tags

V5

Mutation

splice isoform *

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
Notch1 intracellular domain-pcw107

Plasmid

#64621

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Mutation

intracellular domain *

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pCRISPaint-mNeon-BlastR [M1G]

Plasmid

#171805

Purpose

Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mNeonGreen-T2A-BlastR

Use

Gene tagging

Mutation

BlastR: Changed Methionin 1 to Glycine.

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3Basic_ME.1/ApoEpromoter

Plasmid

#51435

Purpose

This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.1 enhancer

Depositor

Sohail Tavazoie

Article

Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

Insert

ME.1+ApoE promoter -980/+(93 bp.) (APOE, ME1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

ME.1 enhancer is fused upstream of ApoE promoter …

Available Since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-EF1α1.1-ChrimsonR-GFP

Plasmid

#122063

Purpose

AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has Service

AAV2

Insert

ChrimsonR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

EF1a（1.1kb short version)

Available Since

March 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-fd [ChrimsonR-tdTomato]

Plasmid

#84483

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. tdTomato is a codon diversified version.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

human synapsin promoter

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pNTK human MBD3 PGK-Neo-pA flox

Plasmid

#52362

Purpose

Targeting vector for Human MBD3 conditional knockout. Also creates hypomorphic alleles.

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

MBD3 (MBD3 Human)

Use

Targeting

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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Hras (G12V)-pcw107

Plasmid

#64603

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

HRAS (HRAS Human)

Use

Lentiviral

Mutation

G12V

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
AAV-Syn-FLPX-rc [ChrimsonR-tdTomato]

Plasmid

#128589

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has Service

AAV8

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato (codon diversified version)

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

Syn

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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TRCN0000338462

Plasmid

#78158

Purpose

shXPO1 targeting CDS

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

XPO1 (XPO1 Human)

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
MEK5 DD(S311D,T315D)-pcw107-V5

Plasmid

#64619

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K5 (MAP2K5 Human)

Use

Lentiviral

Tags

V5

Mutation

S311D, T315D*

Promoter

PGK

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-FOXN1

Plasmid

#101445

Purpose

Donor Vector containing FOXN1 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXN1 (FOXN1 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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