We narrowed to 9,186 results for: mel

1. pAAV-EF1α1.1-FLEX-rc [Chronos-GFP]

   Plasmid

   #84485

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1α, 1.1 kb long (short version)

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. JNK2 WT O/E (MAPK9)-pcw107-V5

   Plasmid

   #64617

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   MAPK9 (transcript variant JNK2-a2) (MAPK9 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   none

   Promoter

   PGK

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

3. FLAG-MKK6 (S207E,T211E)-pcw107

   Plasmid

   #64625

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   MAP2K6 (MAP2K6 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   S207E,T211E

   Promoter

   PGK

   Available Since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

4. myr-FLAG-MEK5-pcw107

   Plasmid

   #64620

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   MAP2K5 (MAP2K5 Human)
   

   Use

   Lentiviral

   Tags

   Flag and myristoylated

   Promoter

   PGK

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pBRPy CAGGS-Mbd3-IRES-PURO

   Plasmid

   #52376

   Purpose

   Mammalian expression vector for constitutive expression of Mbd3

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   Mbd3 (Mbd3 Mouse)
   

   Tags

   2xFlag (DYKDDK)

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. NES-EGFP-PH-BTKx1

   Plasmid

   #183651

   Purpose

   PtdIns(3,4,5)P3 biosensor

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   BTK (BTK Frog, Human)
   

   Tags

   X. laevis map2k1.L(32-44)-EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 2-170

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. TMEM230-WT-IRES(isoform 2)

   Plasmid

   #99564

   Purpose

   mammalian expression of TMEM230 (isoform 2) and ZsGreen

   Depositor

   Han-Xiang Deng

   Article

   Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

   Insert

   TMEM230 (TMEM230 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-CAG-FLPX-rc [ChrimsonR-GFP]

   Plasmid

   #118295

   Purpose

   AAV-mediated expression of ChrimsonR-GFP under the CAG promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   CAG

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. shERK2b-mlpx-neo

   Plasmid

   #65231

   Purpose

   encodes a shRNA against ERK2

   Depositor

   Gerardo Ferbeyre

   Article

   Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

   Insert

   shRNA against ERK2 (MAPK1 Human)
   

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-EF1α1.1-FLPX-rc [ChrimsonR-GFP]

    Plasmid

    #128588

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    EF1α (1.1 kb short version)

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEAHISMRSA0482

    Plasmid

    #96968

    Purpose

    expression of methicillin-resistant Staphylococcus aureus orf 0482

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    MRSA ORF0482
    

    Tags

    N-ter TEV protease cleavable 6His

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-YAP2 (8SA)-pcw107

    Plasmid

    #64637

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    YAP2 (transcript variant 3) (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    8SA*

    Promoter

    PGK

    Available Since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

13. mRFP-FKBP-INPP5E(D556A)

    Plasmid

    #183678

    Purpose

    Recruitable human type IV 5-phosphatase enzyme (catalytically inactive)

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    INPP5E (INPP5E Human)
    

    Tags

    mRFP-FKBP12(1-108)

    Expression

    Mammalian

    Mutation

    C641A, D556A

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221-HES3

    Plasmid

    #101411

    Purpose

    Donor Vector containing HES3 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    HES3 (HES3 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGG369

    Plasmid

    #165606

    Purpose

    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…

    Expression

    Bacterial

    Mutation

    Recoding of BsaI site in the AmpR cassette, two B…

    Promoter

    lacUV2 driving ω-dCas9 and UV5 driving sgRNA

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. TMEM230-WT-IRES(isoform 1)

    Plasmid

    #99558

    Purpose

    mammalian expression of TMEM230 (isoform 1) and ZsGreen

    Depositor

    Han-Xiang Deng

    Article

    Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

    Insert

    TMEM230 (TMEM230 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEAHISMRSA2028

    Plasmid

    #97001

    Purpose

    Expressing MRSA Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase
    

    Tags

    N-ter TEV protease cleavable 6His

    Mutation

    none

    Promoter

    T7

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28-His6-SUMO-Fyn-SH2-AviTag

    Plasmid

    #214202

    Purpose

    Bacterial expression of SH2 domain of the Fyn kinase (B- iso) with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

    Insert

    FYN kinase (FYN Human)
    

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28-His6-SUMO-Fgr-SH2-AviTag

    Plasmid

    #214205

    Purpose

    Bacterial expression of SH2 domain of the Fgr kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

    Insert

    Fgr kinase SH2 domain (FGR Human)
    

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB CRIV Gc Spike H6

    Plasmid

    #240163

    Purpose

    Plasmid for making PiggyBac stable cell line expressing Cristoli virus (CRIV) glycoprotein Gc spike with a C-terminal His6 tag

    Depositor

    Stephen Graham

    Article

    Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

    Insert

    CRIV Gc spike
    

    Use

    Piggybac

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    contains residues 478-906 only

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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