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Showing: 681 - 700 of 15862 results

  1. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-deltaPEST

    Plasmid
    #221118
    Purpose
    FAP tagged STE3-PEST mutant (delta413-470 - N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER
    Depositor
    Allyson O’Donnell
    Article
    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi
    Insert
    MFA-IgKappa-FAPoptim-STE3-deltaPEST
    Use
    Tags
    Expression
    Yeast
    Mutation
    STE3 mutant that lacks the PEST domain in the C-t…
    Promoter
    STE3
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    221118-plasmid-map-sequence-id-442464

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  2. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-K424R

    Plasmid
    #221119
    Purpose
    FAP tagged STE3-K424R mutant (N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER
    Depositor
    Allyson O’Donnell
    Article
    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi
    Insert
    MFA-IgKappa-FAPoptim-STE3-K424R
    Use
    Tags
    Expression
    Yeast
    Mutation
    STE3 mutantK424R
    Promoter
    STE3
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    221119-plasmid-map-sequence-id-442458

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  3. pTipi2.1_Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa

    Plasmid
    #221354
    Purpose
    Mammalian expression of Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa; to be used with Anti-GCN4 [IPI-C11L34] heavy chain (Plasmid 221353) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221354-plasmid-map-sequence-id-436804

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  5. pcDNA3.1-kappa-myc-dL5-2xG4S-TMst

    Plasmid
    #73206
    Purpose
    Expresses myc-dL5(E52D)-TM (PDGFR derived) on the surface of mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    kappa-myc-dL5-2XG4S-TM (MYC Human)
    Use
    Tags
    The FAP is fused with 2 copies of a G4S linker an…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73206-plasmid-map-sequence-id-139721

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  10. pTipi2.1_Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa

    Plasmid
    #221364
    Purpose
    Mammalian expression of Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa; to be used with Anti-His [IPI-HAP1/5.13.22] heavy chain (Plasmid 221363) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221364-plasmid-map-sequence-id-436832

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  14. pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL

    Plasmid
    #73209
    Purpose
    Expresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    kappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Synthetic, Human)
    Use
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73209-plasmid-map-sequence-id-139734

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  17. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

    Plasmid
    #216695
    Purpose
    Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)
    Depositor
    Franck Perez
    Article
    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s
    Inserts
    FIREmate-KDEL
    ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
    Use
    Tags
    FIREtag, Streptavidin Binding Protein (SBP), and …
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216695-plasmid-map-sequence-id-429706

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  18. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid
    #105842
    Purpose
    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mutation
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105842-plasmid-map-sequence-id-393690

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  20. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid
    #105842
    Purpose
    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mutation
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105842-plasmid-map-sequence-id-393690

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Showing: 681 - 700 of 15862 results