We narrowed to 4,407 results for: BRE

1. pcDNA3.1(+)Gα12-133-NLuc

   Plasmid

   #207156

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNA12 (GNA12 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position A(133)F

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330_SAFBgRNA9

   Plasmid

   #196108

   Purpose

   Contains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196107

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   SAFB (Safb Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMeCP2Y pc644

   Plasmid

   #167565

   Purpose

   The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced YFP (pEYFP-N1 vector; CLONTECH Laboratories, Inc.)

   Depositor

   Cristina Cardoso

   Article

   Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

   Insert

   Methyl-CpG binding protein 2 (Mecp2 Rat)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-Mm_Plk5(300-595) PBD domain

   Plasmid

   #136316

   Purpose

   Mammalian expression of mouse Plk5-D300 (PBD domain 300-595) with N-terminal EGFP

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

   Insert

   PLK5 (Plk5 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   fragment 300-595

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-Mm_MyoGEF(WT)

   Plasmid

   #136327

   Purpose

   Mouse MyoGEF-WT in Gateway pENTR

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

   Insert

   PLEKHG6 (Plekhg6 Mouse)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-FRT-TO-Nprot_GC3opt

   Plasmid

   #157730

   Purpose

   mammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nprot_GC3opt (N SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pcDNA5-FRT-TO-Nsp8_GC3opt

   Plasmid

   #157697

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp8_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pcDNA-FRT-TO-Nsp8-Nsp7_GC3opt

   Plasmid

   #157700

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pcDNA5-FRT-TO-FH-Nsp8_GC3opt

   Plasmid

   #157698

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA-PU.1-ETS N236H

    Plasmid

    #124616

    Purpose

    Chimeric ETS domain: N236H

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (ETS1, SPI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 236 altered from asparagine to a histidi…

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-PU.1-ETS N236Q

    Plasmid

    #124615

    Purpose

    Chimeric ETS domain: N236Q

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 236 altered from asparagine to a glutami…

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-PU.1-ETS N236Y

    Plasmid

    #124614

    Purpose

    Chimeric ETS domain: N236Y

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 236 altered from asparagine to a Tyrosine

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti-EF1a-dCas9-VPR-Puro

    Plasmid

    #99373

    Purpose

    3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    (Sp)dCas9-VPR-P2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-TetO-hNGN2-Puro

    Plasmid

    #79049

    Purpose

    Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.

    Depositor

    Kristen Brennand

    Article

    Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

    Insert

    hNGN2-P2A-PuroR (NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TetO

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. CIRTS-control

    Plasmid

    #213788

    Purpose

    CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.

    Depositor

    Timothy Bredy

    Article

    Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:

    Insert

    CIRTS-Calm3-U6-control gRNA
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Syn1, U6

    Available since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. A1-LCD-4D

    Plasmid

    #178672

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with four Asp residues removed (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-4D (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    four Asp residues removed

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. CIRTS-Gas5

    Plasmid

    #213172

    Purpose

    CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and Gas5 guide RNA is expressed under human Syn1 and U6 promoter, respectively.

    Depositor

    Timothy Bredy

    Article

    Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:

    Insert

    CIRTS-Calm3-U6-Gas5 gRNA
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Syn1, U6

    Available since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5-FRT-TO-Nprot-HF_GC3opt

    Plasmid

    #157732

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nprot-HF_GC3opt (N SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pcDNA5-FRT-TO-Nsp13-HF_GC3opt

    Plasmid

    #157716

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

    Plasmid

    #157702

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge