We narrowed to 1,576 results for: PFL

1. LLP779_αGCN4-VP64-CO

   Plasmid

   #211772

   Purpose

   SunTag counterpart binding domain, αGCN4, fused to transcriptional activator VP64, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   aGCN4-VP64
   

   Use

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect VP64 e…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LLP790_αGCN4-EZH2-FL-CO

   Plasmid

   #211784

   Purpose

   SunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SnoopCatcher-KRAB
   

   Use

   Tags

   2xOLLAS

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect KRAB e…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LLP789_αGCN4-DNMT3A-CO

   Plasmid

   #211783

   Purpose

   SunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   aGCN4-DNMT3A
   

   Use

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Last 300 bp codon optimised (CO) to detect DNMT3A…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LLP782_Traptavidin-KRAB-CO

   Plasmid

   #211775

   Purpose

   AviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SpyCatcher-DNMT3A
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect DNMT3A…

   Promoter

   pEF1a and pSV40

   Available since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LLP785_Traptavidin-DNMT3A-CO

   Plasmid

   #211778

   Purpose

   AviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Traptavidin-EZH2
   

   Use

   Tags

   3xV5

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect EZH2 e…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LLP781_SnoopCatcher-KRAB-CO

   Plasmid

   #211774

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Traptavidin-KRAB
   

   Use

   Tags

   3xV5

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect KRAB e…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LLP792_L2_FRT-OCS-tGFP_Red_mCherry_HSP-FlpO

   Plasmid

   #192382

   Purpose

   To test if a single plasmid stably transformed into Arabidopsis can switched from off to on when heat shocked.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::B3RT-OCS-B3RT::Act2::FRT-OCS-FRT::Turbo GFP
   

   Use

   Synthetic Biology

   Tags

   N7

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LLP793_L2_V05_s35S_AND_GFP_pOp6_Flp_CO2_B3

   Plasmid

   #192397

   Purpose

   To test if a single plasmid stably transformed into Arabidopsis can switched from off to on when its AND gate unit is activated by both cell type (cortex) and chemical induction (by DEX).

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   35S::FRT-OCS-FRT::B3RT-OCS-B3RT::Turbo GFP
   

   Use

   Synthetic Biology

   Tags

   N7

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LLP722_pSP15

   Plasmid

   #192435

   Purpose

   To test the relative expression strength of the TCTP1 promoter

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   TCTP1::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LLP720_pSP13

    Plasmid

    #192433

    Purpose

    To test the relative expression strength of the 35S promoter

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    35S::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LLP721_pSP14

    Plasmid

    #192434

    Purpose

    To test the relative expression strength of the Act2 promoter

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LLP743_L2pV1_OR_B3RT_FRT_Rluc_0I_lacZ

    Plasmid

    #192387

    Purpose

    To act as the 0-input state of the OR gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LLP689_L2_1IF2_FRT-OCS_Rluc_C1_FlpF2

    Plasmid

    #192412

    Purpose

    To act as a 1-input state of the split-AND gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LLP690_L2_1IF1_FRT-OCS_Rluc_FlpF1_C1-NLS

    Plasmid

    #192413

    Purpose

    To act as a 1-input state of the split-AND gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LLP752_L2pV1_AND_B3RT_FRT_Rluc_2IBF_lacZ

    Plasmid

    #192396

    Purpose

    To act as a 2-input state of the AND gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LLP747_L2pV1_OR_B3RT_FRT_Rluc_2IBF_lacZ

    Plasmid

    #192391

    Purpose

    To act as a 2-input state of the OR gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LLP675_L2_pV01_1I_vox-OCS-Rluc_TCTP_Vika_lacZ

    Plasmid

    #192427

    Purpose

    To act as the on state for testing of the alt Vika recombinase.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::vox-OCS-vox::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LLP772_L2pV1_NOR_B3RT_FRT_Rluc_2IBF_lacZ

    Plasmid

    #192432

    Purpose

    To act as a 2-input state of the NOR gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-B3RT-Rluc-B3RT-FRT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LLP762_L2pV1_OR-OCS_TCTP-Flp_s35S-Cre

    Plasmid

    #192421

    Purpose

    To act as the 2-input state of the OR gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-loxP-OCS-FRT-loxP::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LLP765_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_1IF_lacZ

    Plasmid

    #192409

    Purpose

    To act as a 1-input state of the A NIMPLY B gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    B3RT-Act2-B3RT::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge