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FUW-KM

Plasmid

#20332

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Klf4-F2A-cMyc

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pRL1383a

Plasmid

#70692

Purpose

RSF1010-based vector with extensive polylinker, and resistance to Strep and Spec. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.

Depositor

Peter Wolk

Article

Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

Type

Empty backbone

Use

Bacterial shuttle vector

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual- GST-TEV-TBK1

Plasmid

#208875

Purpose

For expression in insect cells of TBK1

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

TBK1 (TBK1 Human)

Tags

GST-TEV

Expression

Insect

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAC155-pCR8-sgExpression

Plasmid

#49045

Purpose

U6-driven sgRNA expressing cassette on a gateway donor vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-MAFB-sg

Plasmid

#194718

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hMAFB

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1 (MAFB Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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FoxFTVC+bpFOG_Cdkn1b.a

Plasmid

#107523

Purpose

Negative regulator of cell cycle progression by G1/S transition inhibition

Depositor

Lionel Christiaen

Article

Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

Insert

Cdkn1b.a

Promoter

TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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FoxFTVC+bpFOG_Cdkn1b.b

Plasmid

#107524

Purpose

Negative regulator of cell cycle progression by G1/S transition inhibition

Depositor

Lionel Christiaen

Article

Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

Insert

Cdkn1b.b

Promoter

TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pPGKpuro

Plasmid

#11349

Depositor

Rudolf Jaenisch

Article

Tucker et al Genes Dev. 1996 Apr 15. 10(8):1008-20.

Insert

pgk-1 5' and 3' regions (Pgk1 Mouse)

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD

Plasmid

#84475

Purpose

Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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Eef1a-1955/-1>nls::Cas9::nls

Plasmid

#59987

Purpose

Eef1a (EF1alpha) promoter driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Eef1a (EF1alpha) -1955/-1

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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hNav1.8_pcDNA3.1

Plasmid

#182847

Purpose

Expresses hNav1.8 in mammalian cells

Depositor

Paul DeCaen

Article

McCollum et al Cell Rep. 2022 Aug 23;40(8):111248. doi:

Insert

SCN10A (SCN10A Human)

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Nr5a1

Plasmid

#41081

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Nr5a1 (Nr5a1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO + minimal CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD_IM

Plasmid

#84479

Purpose

Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD_IM

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Dnmt3a_IM

Plasmid

#84478

Purpose

Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a_IM

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMF-TetTight

Plasmid

#193288

Purpose

Lentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgene

Depositor

Rudolf Jaenisch

Article

Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

Insert

rtTA-P2A-tTS

Use

Lentiviral

Expression

Mammalian

Promoter

EF‐1α

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-oct4

Plasmid

#20323

Depositor

Rudolf Jaenisch

Article

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

oct4 (Pou5f1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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pMF-FU_TetO_GW_PPARG2

Plasmid

#193289

Purpose

Lentiviral construct that expresses a dox-inducible human PPARG2

Depositor

Rudolf Jaenisch

Article

Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

Insert

PPARG2 (PPARG2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAC103-PBNeo-U6-sgRNA-Expression

Plasmid

#48228

Purpose

sgRNA expression on PiggyBac vector with Neo-selectable marker

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only

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pBMN-HA-SINTBAD

Plasmid

#210209

Purpose

For mammalian expression of SINTBAD (Internal ref. SMC2249)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

TBKBP1 (TBKBP1 Human)

Expression

Mammalian

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAC150-PBLHL-4xHS-EF1a-DEST

Plasmid

#48234

Purpose

PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

Piggybac

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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