We narrowed to 2,924 results for: fas

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lenti SYN-dCas9-KRAB-MeCP2

Plasmid

#155365

Purpose

Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

Depositor

Jeremy Day

Article

Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

Insert

dCas9-KRAB-MeCP2

Use

CRISPR and Lentiviral

Tags

3x FLAG and 7x His

Expression

Mammalian

Promoter

SYN

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1-Q61L

Plasmid

#12981

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Rac1 constitutively active (RAC1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q61L Constitutively active

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42-T17N

Plasmid

#12976

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Cdc42 DN (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T17N Dominant negative

Promoter

CMV

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42-Q61L

Plasmid

#12986

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Cdc42 constitutively active (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q61L Constitutively active

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1-T17N

Plasmid

#12982

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Rac1 DN (RAC1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T17N Dominant negative

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pBBR1.cas9-NT

Plasmid

#197871

Purpose

Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)

Depositor

Baojun Wang

Article

Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72

Inserts

trancCRNA
Cas9
crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCR1162

Plasmid

#111098

Purpose

Expresses FANCA_-_89883048.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCA gRNA (FANCA Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1161

Plasmid

#111097

Purpose

Expresses FANCA_-_89883045.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCA gRNA (FANCA Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42-wt

Plasmid

#12975

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Cdc42 (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42337

Purpose

Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pSBbiRP Hu Full Length CD142 (Tissue Factor; F3)

Plasmid

#183254

Purpose

Expression of full length human tissue factor (CD142; F3) in a Sleeping Beauty transposon vector

Depositor

Alexander McLellan

Article

Muhsin-Sharafaldine et al Oncotarget. 2016 Aug 30;7(35):56279-5629

Insert

Tissue factor (F3 Human)

Expression

Mammalian

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJC-repo1.9kb-TALE4-VP64-P10

Plasmid

#104612

Purpose

Expresses TALE4 under the control of a 1.9kb repo enhancer element

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

1.9kb repo enhancer element (repo Fly)

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMX-DivisionRecorder

Plasmid

#179446

Purpose

Retroviral expression vector containing the DivisionRecorder cassette

Depositor

Ton Schumacher

Article

Bresser et al Nat Immunol. 2022 May;23(5):791-801. doi

Insert

GFP-P2A-511-CrePI-511

Use

Retroviral

Promoter

LTR

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-hFADD-FLAG

Plasmid

#31814

Depositor

Astar Winoto

Article

Young et al J Biol Chem. 2011 Feb 25;286(8):6521-31.

Insert

FADD (FADD Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNATM3.1/V5-His TOPO-PKCi-K274W

Plasmid

#55690

Purpose

expresses dominant negative human PKCi tagged with V5

Depositor

Pedro Salas

Article

Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

Insert

Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)

Tags

V5/His

Expression

Mammalian

Mutation

A120E and K274W

Promoter

CMV

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only
pcDNATM3.1/V5-His TOPO-PKCi-A120E

Plasmid

#55687

Purpose

expresses constitutively active human PKCi tagged with V5

Depositor

Pedro Salas

Article

Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

Insert

Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)

Tags

V5/His

Expression

Mammalian

Mutation

A120E

Promoter

CMV

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1-nsp7-FLAG

Plasmid

#201027

Purpose

Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

8xHis and FLAG

Expression

Mammalian

Promoter

T7 promoter

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR.sgKras.9

Plasmid

#91894

Purpose

sgRNAs targeting mouse Kras. 3rd generation lentiviral backbone.

Depositor

Wen Xue

Article

Mou et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

Kras sgRNA

Use

Lentiviral

Promoter

U6

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pJC-ase0.8kb-TALE3-VP64-P10

Plasmid

#104610

Purpose

Expresses TALE3 under the control of a 0.8kb ase enhancer element

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

0.8kb ase enhancer element (ase Fly)

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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