We narrowed to 5,513 results for: mcs

1. pTS105 pHAGE2 CMVtetO2 MCS-TEV-22xGS-GFP

   Plasmid

   #199358

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TEV-EGFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPDK3902(TRV-RNA2-pPEBV-MCS-AtGly-tRNA)

   Plasmid

   #149278

   Purpose

   Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter and AtGly-tRNA mobile RNA sequence
   

   Use

   T-dna vector

   Mutation

   T2318C (DNA) in PEBV coat protein sequence

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-1417 (No intron)

   Plasmid

   #69898

   Purpose

   Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1 (stw, ZKSCAN1 Human)
   

   Expression

   Mammalian

   Mutation

   F169L in ZKSCAN1

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-SynI-CreOn-FlpOff-MCS-P2A-EGFP

   Plasmid

   #176276

   Purpose

   Viral vector for co-expression of a CDS of interest and EGFP in cells expressing Cre AND NOT Flp driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   MCS-P2A-EGFP
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   human Synapsin I

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS108 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-GFP

   Plasmid

   #199361

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-SUMOstar-EGFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDest-Tol2 (Chien)-Krt4:Gal4VP16:tdTomato:5xUAS_MCS

   Plasmid

   #105649

   Purpose

   tdTomato expression in differentiated keratinocytes of the periderm layer and during early larval stages also in the basal layer of the epidermis

   Depositor

   Sandra Rieger

   Insert

   Krt4:Gal4VP16:tdTomato:5xUAS_MCS
   

   Expression

   Mammalian

   Available Since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-1417 (No intron)

   Plasmid

   #69904

   Purpose

   Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1 (ZKSCAN1 Human)
   

   Expression

   Mammalian

   Mutation

   F169L in ZKSCAN1

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pALS186 (ttTi5605-MCS loxP::C.br.unc-119(+)::loxP)

   Plasmid

   #129539

   Purpose

   modified pCFJ151 for C. elegans mosSCI at ttTi5605 or compatible sites; floxed C. br. unc-119(+) selection

   Depositor

   Arneet Saltzman

   Article

   Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

   Type

   Empty backbone

   Use

   Cre/Lox; Mos1-mediated single copy insertion (mos…

   Expression

   Worm

   Available Since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Hy_pMT Laccase2 MCS-ZKSCAN1 548-1417 (No intron)

   Plasmid

   #69887

   Purpose

   Expresses a miniature version of the ZKSCAN1 circular RNA in Drosophila

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1 (stw, ZKSCAN1 Human)
   

   Expression

   Insect

   Mutation

   F169L in ZKSCAN1

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-U6-DR36(DjCas13d-SapI)-CMV-intron-MCS-pA

    Plasmid

    #233031

    Purpose

    To express a DjCas13d-compatible gRNA

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    DjCas13d-compatible gRNA expression cassette
    

    Use

    AAV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSAH46 - MosTI [unc-119(partial) | MCS | gfp]

    Plasmid

    #200366

    Purpose

    Cloning vector for mosTI(unc-119, gfp). MCS or Golden Gate.

    Depositor

    Christian Froekjaer-Jensen

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSAH45 - MosTI [unc-119(partial) | MCS | wrmScarlet]

    Plasmid

    #200365

    Purpose

    Cloning vector for mosTI(unc-119, wrmScarlet). MCS or Golden Gate.

    Depositor

    Christian Froekjaer-Jensen

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-MCS-PrP (1-22)-BioID2-HA-13 x Linker-GPI-PSS

    Plasmid

    #194751

    Purpose

    To fuse your protein of interest to the C-terminus of GPI-anchored-BioID2

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Type

    Empty backbone

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TetO-FUW-FLAG-NLS-EnR-MCS_N-term

    Plasmid

    #136884

    Purpose

    Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    engrailed repressor domain (en Fly)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. TetO-FUW-FLAG-NLS-VP64-MCS_N-term

    Plasmid

    #136886

    Purpose

    Doxycycline-inducible vector backbone containing the VP64 activator domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    VP64 activator domain
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. TetO-FUW-FLAG-NLS-KRAB-MCS_N-term

    Plasmid

    #136888

    Purpose

    Doxycycline-inducible vector backbone containing the KRAB repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    KRAB repressor domain
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDF1-MCS2-EF1-Puro-RGS7-R44C+V56C

    Plasmid

    #113730

    Purpose

    Express double mutant RGS7- R44C+V65C in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

    Insert

    RGS7 (RGS7 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R44C and V56C

    Promoter

    CMV

    Available Since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

18. MSCV-(mcs) human CSF1 full length-IRES-Puro

    Plasmid

    #86797

    Purpose

    retroviral expression of CSF-1

    Depositor

    Martine Roussel, Charles Sherr

    Article

    Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

    Insert

    CSF-1 (CSF1 Human)
    

    Use

    Retroviral

    Tags

    IRES-Puro

    Expression

    Mammalian

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTS107 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-GFP

    Plasmid

    #199360

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-SUMOEu-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSB-CMV-MCS-puro PM-mKate2-P2A-GEM-LTB4

    Plasmid

    #202643

    Purpose

    Expresses the LTB4 sensor GEM-LTB4 along with the plasma membrane targeted mKate2 tag in mammalian cells

    Depositor

    Balázs Enyedi

    Article

    Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

    Insert

    PM-mKate2-P2A-GEM-LTB4
    

    Tags

    PM-mKate2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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