We narrowed to 3,106 results for: ogn
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Plasmid#109033PurposeD16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
TagsFlag TagExpressionBacterialPromoterT7Available SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag H588A
Plasmid#109032PurposeH588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
TagsFlag TagExpressionBacterialPromoterT7Available SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS2_HindIII
Plasmid#65666PurposeFLAG-HA expression vector with RBPMS2 carrying mutation to introduce HindIII siteDepositorInsertRBPMS2 (RBPMS2 Human)
TagsFLAG HAExpressionMammalianMutationintroduced HindIII site (final goal to exchange F…PromoterCMV, 2x TetAvailable SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K570A
Plasmid#14102DepositorAvailable SinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K533A
Plasmid#14100DepositorAvailable SinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2Q545A
Plasmid#14101DepositorAvailable SinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K533AQ545A
Plasmid#14103DepositorInsertAgo2 (AGO2 Human)
TagsFLAG/HAExpressionMammalianMutationchanged Lys533 to Ala, Gln545 to AlanineAvailable SinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
LLP1013
Plasmid#239935PurposeCsy4 recognition sequences (Cog) as Level-0 part (Golden Gate cloning)DepositorInsertCsy4 recognition sequence
UseSynthetic BiologyMutationN/AAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19_21 tRNA genes
Plasmid#242430PurposeIn vitro transcription of 21 tRNAs after Nt.BspQI treatment. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene.DepositorInsert21 tRNA genes with a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene
ExpressionBacterialAvailable SinceAug. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEW107
Plasmid#232796PurposeCOMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) in pBIG2abDepositorTagsHis6-3xFLAG, TwinstrepExpressionInsectMutationySet1(762-1080)Promoterpolyhedrin promoterAvailable SinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEM-601[insert(+1) TA-rich]
Plasmid#221196Purpose601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601DepositorInsert601[insert(+1)TA-rich]
UseUnspecifiedMutation601 has an additional mutation added 22 bp from d…Available SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-ScChd1[R126A/R130A]
Plasmid#221197PurposeScChd1-aa118-1274 chromatin remodeler with R126A and R130A mutationsDepositorInsertScChd1 (aa118-1274)
Tags6xHis-tag (Precission protease cleavable)ExpressionBacterialMutationR126A, R130APromoterT7Available SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-ScChd1[∆ChEx]
Plasmid#221199PurposeScChd1-aa142-1274 chromatin remodeler with ChEx domain deletedDepositorInsertScChd1 (aa142-1274)
Tags6xHis-tag (Precission protease cleavable)ExpressionBacterialMutationdeleted amino acids 1-117 and 1275-1468Available SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SCML2_3
Plasmid#220878PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceJune 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-SHmOCR
Plasmid#206874PurposeEmpty Gateway pDEST vector for bacterial expression with Super His - Monomeric OCR fusionDepositorTypeEmpty backboneTagsHSV + 6xHis and SuperHis (14xHis) + monomeric OCR…ExpressionBacterialPromoterT7Available SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ1A
Plasmid#198168PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 μ1A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 μ1A
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastPromoterADH1Available SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_motif_scram
Plasmid#149397PurposeP. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motifDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutationUpstream motif recognized by Cas1-2/3 during spac…PromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8-Mcl1_deltaTM
Plasmid#187118Purposeinsect cell expression of Mcl1-deltaTMDepositorAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8-DDIT4_delta8
Plasmid#187117Purposeinsect cell expression of DDIT4-delta1-8DepositorAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_R4187C
Plasmid#187141PurposeEntry cloneDepositorAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only