We narrowed to 6,443 results for: plasmid dna

1. pcDNA3.1-α-COPI-WD40

   Plasmid

   #180758

   Purpose

   Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 (residues 1-327)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

   Insert

   α-COPI-WD40
   

   Use

   Tags

   C-terminal strep tag

   Expression

   Mammalian

   Mutation

   Encodes residues 1-327; mutations: Leu181Lys, Leu…

   Promoter

   CMV

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5-FRT-TO-Nsp2-HF

   Plasmid

   #157684

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp2-HF (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pcDNA-FRT-TO-Nprot_GC3opt

   Plasmid

   #157730

   Purpose

   mammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nprot_GC3opt (N SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pcDNA5-FRT-TO-Nsp8_GC3opt

   Plasmid

   #157697

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp8_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pcDNA5-FRT-TO-FH-Nsp8_GC3opt

   Plasmid

   #157698

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pcDNA3-sACE2-T92Q

   Plasmid

   #145156

   Purpose

   Mammalian expression plasmid for soluble ACE2 (protease domain) T92Q glycosylation mutant

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-MS2-HA-HshnRNPH1_185-449-del291-361_Z

   Plasmid

   #148123

   Purpose

   Mammalian Expression of HshnRNPH1_185-449-del291-361

   Depositor

   Elisa Izaurralde

   Insert

   HshnRNPH1_185-449-del291-361 (HNRNPH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAMTA2.1.0-gDNA

   Plasmid

   #132456

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   CAMTA2 (CAMTA2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMBD1.1.0-gDNA

   Plasmid

   #132444

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   MBD1 (MBD1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3.0_nucLAMA-G97 IRES mYFP

    Plasmid

    #130713

    Purpose

    Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and mYFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-G97-NLS IRES mYFP
    

    Use

    Tags

    NLS and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA3.0_nucLAMA-F98 IRES mYFP

    Plasmid

    #130712

    Purpose

    Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and mYFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-F98-NLS IRES mYFP
    

    Use

    Tags

    NLS and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1(+)/AAA-nLuc-3XFLAG

    Plasmid

    #127306

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AAA start codon (Negative control plasmid)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(+)/GGG-nLuc-3XFLAG

    Plasmid

    #127307

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from GGG start codon (Negative control plasmid)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.2 mir-1-1 reporter mir50-wt

    Plasmid

    #46719

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir50-wt
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.2 mir-1-1 reporter mir50-7

    Plasmid

    #46716

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir50-7
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.2 mir-1-1 reporter mir50-5

    Plasmid

    #46714

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir50-5
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.2 mir-1-1 reporter mir50-4

    Plasmid

    #46713

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir50-4
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.2 mir-1-1 reporter mir50-3

    Plasmid

    #46712

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir50-3
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.2 mir-1-1 reporter mir50-2

    Plasmid

    #46711

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir50-2
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.2 mir-1-1 reporter mir50-1

    Plasmid

    #46710

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir50-1
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge