We narrowed to 10,563 results for: ESP

1. PCDF Bravo-PotH-OL1del

   Plasmid

   #216749

   Purpose

   Study the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.

   Depositor

   Gordon Laurie

   Article

   Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

   Insert

   potH (potH E. coli (Bacteria))
   

   Tags

   10x His

   Expression

   Bacterial

   Mutation

   Outer loop 1 (OL1) with the sequence of [FKISLAEM…

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PCDF Bravo-PotH-OL2del

   Plasmid

   #216750

   Purpose

   Study the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL1 in its interaction with exogenous peptides.

   Depositor

   Gordon Laurie

   Article

   Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

   Insert

   potH (potH E. coli (Bacteria))
   

   Tags

   10x His

   Expression

   Bacterial

   Mutation

   Outer loop 2 (OL2) with the sequence of [WMGILKNN…

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PCDF Bravo-PotH-OL3del

   Plasmid

   #216751

   Purpose

   Study the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 3 (OL3) by examining the variant lacking OL1 in its interaction with exogenous peptides.

   Depositor

   Gordon Laurie

   Article

   Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

   Insert

   potH (potH E. coli (Bacteria))
   

   Tags

   10x His

   Expression

   Bacterial

   Mutation

   Outer loop 3 (OL3) with the sequence of [ELLGGPDS…

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET41-FeoB-OL1del

   Plasmid

   #216741

   Purpose

   Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.

   Depositor

   Gordon Laurie

   Article

   Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

   Insert

   feoB (PA4358 P. aeruginosa (Bacteria))
   

   Tags

   8x His

   Expression

   Bacterial

   Mutation

   Outer loop 1 (OL1) with the sequence of [INIGGALQ…

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR

   Plasmid

   #207135

   Purpose

   DNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-in

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)
   

   Expression

   Mammalian

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-U6-DCK-Hygro

   Plasmid

   #202409

   Purpose

   gRNA expression vector for DCK

   Depositor

   Morito Kurata

   Article

   Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

   Insert

   guide RNA (DCK Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Dck

   Promoter

   U6

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204214

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Deletion of DNA binding domain (delDBD)

   Promoter

   TetON

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204191

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Deletion of R162

   Promoter

   TetON

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G

   Plasmid

   #204213

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Point mutation on I207M

   Promoter

   TetON

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204211

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Point mutation on Q175* (*termination)

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204210

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Point mutation on R178* (*termination)

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G

    Plasmid

    #204212

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NK2 homeobox 1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Point mutation on I207F

    Expression

    Mammalian

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX4T3-GST-PolB(T304I)

    Plasmid

    #177133

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CIRC

    Plasmid

    #186343

    Purpose

    Contains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)

    Depositor

    Giovanni Blandino

    Article

    Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

    Insert

    VEGFA (VEGFA Human)
    

    Expression

    Mammalian

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX4T3-GST-PolB(K72A)

    Plasmid

    #177132

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLentiGuide-PolB1-puro

    Plasmid

    #177146

    Purpose

    Lentiviral vector expressing PolB-gRNA-1 and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolBgRNA1 (POLB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX-Flag-PolB(T304I)-Puro

    Plasmid

    #177143

    Purpose

    Lentiviral vector expressing Flag-PolB(T304I) and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIFU002

    Plasmid

    #174299

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    HA and cMyc

    Expression

    Yeast

    Mutation

    N343Q, N501Y

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIFU001

    Plasmid

    #174298

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    HA and cMyc

    Expression

    Yeast

    Mutation

    N343Q, E484K

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETM33_Nsp3e_NAB

    Plasmid

    #156473

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3e_NAB (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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