We narrowed to 7,206 results for: SIM

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pVM2-GoPGF-HD1

Plasmid

#234909

Purpose

This all-in-one vector is used to specifically silence the GoPGF and HD1 gene via virus-induced gene silencing (VIGS), simultaneously.

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

VIGS fragements of GoPGF and GhHD1

Expression

Plant

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-NT-BmNG

Plasmid

#232204

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy-mNeonGreen

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mNeongreen

Expression

Bacterial

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-NT-BmSc

Plasmid

#232205

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy-mScarlet

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mScarlet

Expression

Bacterial

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-BmSc

Plasmid

#232208

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-BSD-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mScarlet

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-NT-PmSc

Plasmid

#232548

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mScarlet

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mScarlet

Expression

Bacterial

Mutation

None

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-hsAgo2

Plasmid

#221718

Purpose

Expression of human Ago2 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2 (AGO2 Human)

Use

Fish expression

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_Seq17_VS33_4.26_ChimeraIntron_3NLS_RFP_WPRE_polyA

Plasmid

#220225

Purpose

AAV-STARR-seq validation vector containing VS33 enhancer

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Insert

Enhancer (VS33)

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA

Plasmid

#174897

Purpose

ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sf GFP

Expression

Mammalian

Mutation

150TAA in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA

Plasmid

#174898

Purpose

opal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TGA in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR4 Ta-SRO1 JN177

Plasmid

#192546

Purpose

pENTR plasmid Triticum aestivum SRO1, full CDS, cultivar JN177, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1), cultivar Jinan 177 (JN177)

Use

Gateway entry vector

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR4 Ta-sro1 SR3

Plasmid

#192547

Purpose

pENTR plasmid Triticum aestivum sro1, full CDS, cultivar SR3, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1), cultivar Shanrong No. 3 (SR3)

Use

Gateway entry vector

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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NK10 SFFV-mCherry-SG(s70587-3xMS2)SG-EGFP (FLP-IN)

Plasmid

#191165

Purpose

Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the coding sequence in mammalian cells. Similar to EK0438, but with MS2 sequences directly after the sensor sequence, rather than 3' UTR

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587-3xMS2:EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_4xT790_28bp

Plasmid

#114720

Purpose

4 x 28 bp repeats of the EGFR WT allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Expression

Bacterial

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_T790_28bp

Plasmid

#114722

Purpose

28 bp fragment of the WT EGFR allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Expression

Bacterial

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q48T)

Plasmid

#112725

Purpose

This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48T)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48T

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P51A)

Plasmid

#112728

Purpose

Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P51A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P51A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mCherry Gamma 3

Plasmid

#65234

Purpose

To study the role of the differential rates of Gβγ translocation in modulating signaling dynamics in a cell

Depositor

Narasimhan Gautam

Article

Giri et al Biophys J. 2014 Jul 1;107(1):242-54. doi

Inserts

mcherry
Gng3 (Gng3 Mouse)

Tags

6-His tag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Alpha-0 Dronpa

Plasmid

#44193

Depositor

Narasimhan Gautam

Article

Chisari et al J Biol Chem. 2007 Aug 17;282(33):24092-8

Insert

G protein alpha 0 (Gnao1 Rat)

Tags

Dronpa

Expression

Mammalian

Mutation

Introduced KpnI and SpeI sites between 92nd and 9…

Promoter

CMV

Available Since

April 14, 2015

Availability

Academic Institutions and Nonprofits only
pCS2+-FLAG3-drAgo2DY-EF

Plasmid

#221716

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D651E and Y680F in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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PH Akt-Venus

Plasmid

#85223

Purpose

Venus tagged PH domain of Akt for use as a PIP3 sensor

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Insert

PH AKT(1-148) (AKT1 Human)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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