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Nav1.8 Na+ channel scFv [N134/12]

Plasmid

#190531

Purpose

Mammalian Expression of Nav1.8 Na+ channel scFV. Derived from hybridoma N134/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Nav1.8 Na+ channel (Rattus norvegicus) recombinant scFV (Scn10a Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-EVA1ext-hFcIgG3-IRES-YFP

Plasmid

#64926

Purpose

To express extracellular portion of EVA1 (mouse) fused with human Fc(IgG3)

Depositor

Pau Serra

Article

Garabatos et al Monoclon Antib Immunodiagn Immunother. 2

Insert

mouse EVA1 fused with human FcIgG3 (Mpzl2, IGHG3 Human, Mouse)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Promoter

LTR

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1 SIRT1-deltaExon2

Plasmid

#105671

Purpose

Mammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1 (Sirt1 Mouse)

Expression

Mammalian

Mutation

Synonymous base changes not affecting the protein…

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-7 [N275/14R]

Plasmid

#177526

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-7 (Mouse). Derived from hybridoma N275/14.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Synaptotagmin-7 (Mus musculus) recombinant mouse monoclonal antibody (Syt7 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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p21-S208C/S242R/M694V-MEFV

Plasmid

#134706

Purpose

Lentiviral expression of human MEFV S208C/S242R/M694V after doxycycline administration

Depositor

Thomas Henry

Article

Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Mutation

p.S208C/S242R/M694V

Promoter

TRE2 (Dox-inducible)

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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R620-M21-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.429

Plasmid

#170200

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.429 variant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) B.1.429 (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

S13I, W152C, L452R, D614G

Promoter

CMV51

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX

Plasmid

#112762

Purpose

express GFP-4EBP1-Rheb15(CAAX mutation)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-GFP-4EBP1-Rheb15 (CAAX mutant) (EIF4EBP1 Human)

Use

Lentiviral

Tags

Rheb15(CAAX mutant)

Mutation

CAAX mutation to prevent lipid localization

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

Plasmid

#85821

Purpose

iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Article

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

Zf Ubi::cMyc oDam_f_GFPoNLS

Use

Tol2 based vector

Tags

cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…

Mutation

L122A. Codon optimization compatible to work in M…

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe 3XFLAG-S37A-GATA6-3XAU1 puro

Plasmid

#72608

Purpose

For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#37 to alanine mutation

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Mutation

changed serine37 to alanine

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-eHGT_78h-Cre_PEST

Plasmid

#231791

Purpose

Expresses Cre in excitatory neurons; Cre expression is attenuated by PEST sequence.

Depositor

Karl Deisseroth

Article

Drexler et al Cell. 2025 Aug 21;188(17):4640-4657.e30.

Insert

Cre

Use

AAV

Tags

PEST

Promoter

minBetaGlobin

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gnas

Plasmid

#245994

Purpose

Express HA N-terminal tagged mouse Galphas transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnas (Gnas Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pSPcas9(BB)-2A-Puro V2.0 sgCHMP2B-2 aauucccaaaugaagauggc

Plasmid

#231998

Purpose

Expression of an sgRNA targeting CHMP2B, Cas9, and a PuroR marker

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B-targeted sgRNA (CHMP2B Human)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only
Anti-PICK1 [L20/26R]

Plasmid

#222149

Purpose

Mammalian Expression Plasmid of anti-PICK1 (Human). Derived from hybridoma L20/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-PICK1 (Homo sapiens) recombinant mouse monoclonal antibody. (PICK1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

Plasmid

#223323

Purpose

Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

Depositor

Jonathan Arias

Article

Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

Insert

SpCas9 and sgRNA-T2 (cas9 )

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-12 [N277/26R]

Plasmid

#225386

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-12 (Mouse) IgG2a R-mAb. Derived from hybridoma N277/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Synaptotagmin-12 (Mus musculus) recombinant (Mouse) monoclonal antibody. (Syt12 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-MMP9 [L51/73R]

Plasmid

#225373

Purpose

Mammalian Expression Plasmid of anti-MMP9 (Rat) IgG2a R-mAb. Derived from hybridoma L51/73.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-MMP9 (Rattus norvegicus) recombinant (Mouse) monoclonal antibody. (Mmp9 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-NALCN [N185/7R-2b]

Plasmid

#225412

Purpose

Mammalian Expression Plasmid of anti-NALCN (Rat) IgG2b R-mAb. Derived from hybridoma N185/7.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-NALCN (Rattus norvegicus) recombinant (Mouse) monoclonal antibody. (Nalcn Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-12 [N277/44R]

Plasmid

#225387

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-12 (Mouse) IgG2a R-mAb. Derived from hybridoma N277/44.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Synaptotagmin-12 (Mus musculus) recombinant (Mouse) monoclonal antibody. (Syt12 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-6 [N270/28R]

Plasmid

#225385

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-6 (Mouse) IgG2a R-mAb. Derived from hybridoma N270/28.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Synaptotagmin-6 (Mus musculus) recombinant (Mouse) monoclonal antibody. (Syt6 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-3 [N278/16R]

Plasmid

#225388

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-3 (Mouse) IgG2a R-mAb. Derived from hybridoma N278/16.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Synaptotagmin-3 (Mus musculus) recombinant (Mouse) monoclonal antibody. (Syt3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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