We narrowed to 12,230 results for: nsf

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mNG-sfTq2

Plasmid

#124218

Purpose

Cytoplasmic mNeongreen superfolder mTurquoise2 tandem, ~60 % energy transfer. pNM044 - pSAV057-mNG-sfTq2

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

mNG-sfTq2

Expression

Bacterial

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn-FLEX.iGABASnFR.F102G.mRuby3

Plasmid

#112170

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Tags

mRuby3

Mutation

F102G

Promoter

Synapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eGFP-L10a

Plasmid

#166605

Purpose

Encodes Cre-dependent eGFP-L10a under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

Insert

eGFP-L10a

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-GeneTRAP

Plasmid

#218184

Purpose

To KO and genetrap mouse Hevin simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-N-HATag

Plasmid

#218185

Purpose

To tag Hevin with HA at the N-terminal

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1 KO

Plasmid

#218182

Purpose

To KO Hevin in astrocytes

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-Vmn1r78

Plasmid

#133413

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 78

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-vmn1r78 (Vmn1r78 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2_VP1_RaPID_r1c3

Plasmid

#65723

Purpose

Produce random mCherry insertion in AAV2 VP3 region of VP1; ITRs in VP1 only

Depositor

Junghae Suh

Article

Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

Insert

mCherry

Use

AAV

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pICH47732:PtFCP:ShBle

Plasmid

#104893

Purpose

Expresses ShBle with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation.

Depositor

Thomas Mock

Article

Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

Insert

ShBle

Use

Phaeodactylum; golden gate assembly

Tags

PtFCP promoter and terminator

Available Since

Dec. 28, 2017

Availability

Academic Institutions and Nonprofits only

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Syn:NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEcs(ENLYFQ/M)-tTA

Plasmid

#135462

Purpose

Neuronal expression of uFLARE TF component

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEVcs(ENLYFQ/M)-tTA

Use

AAV

Tags

HA, flag, VP16

Expression

Mammalian

Promoter

Synapsin

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iGABASnFR.R205A

Plasmid

#112162

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.R205A

Use

AAV

Mutation

R205A (no GABA binding)

Promoter

hSynapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-Vmn1r86

Plasmid

#133415

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 86

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-vmn1r86 (Vmn1r86 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP5g-P2A-Vmn1r58

Plasmid

#133418

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 58

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP5g-P2A-PAmCherry-Vmn1r58 (Vmn1r58 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-SYN-TdTomato-RAB11

Plasmid

#203732

Purpose

AAV vector plasmid expressing human RAB11 fused to TdTomato under the human synapsin (SYN) promoter

Depositor

Richard Eva, Bart Nieuwenhuis

Insert

RAB11 (RAB11A Human)

Use

AAV

Tags

TdTomato

Expression

Mammalian

Promoter

Human synapsin promoter

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pNTI826

Plasmid

#216634

Purpose

cpTurboID (V5) for AAV production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

cpTurboID

Use

AAV

Tags

V5

Expression

Mammalian

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pmEGFP-N1 SYS1

Plasmid

#175092

Purpose

SYS1-mEGFP can be transfected in mammalian cells

Depositor

Juan Bonifacino

Article

Ishida et al J Cell Biol. 2019 Nov 4;218(11):3681-369

Insert

SYS1 (SYS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS

Plasmid

#199347

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP-3C

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMVS-V1

Plasmid

#183094

Purpose

Basic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus

Depositor

Lars Angenent, Bastian Molitor

Article

Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

Inserts

pME2001
thermostable neomycin phosphotransferase gene

Use

Bacterial propagation and conjugative

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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GFAP-Archon-EGFP

Plasmid

#187979

Purpose

Expresses the fluorescent voltage indicator Archon under the GFAP promoter

Depositor

Chris Dulla

Article

Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

Insert

Archon1-EGFP

Use

AAV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iGABASnFR.F102Y.Y137L

Plasmid

#112161

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Use

AAV

Mutation

F102Y.Y137L

Promoter

hSynapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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