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pGL3-Dusp6_2KBpromoter

Plasmid

#32666

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Dusp6 (dusp6 Zebrafish)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag TAOK3

Plasmid

#20647

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TAOK3 (TAOK3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag TYK2

Plasmid

#20660

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TYK2 (TYK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag AKT3

Plasmid

#20423

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AKT3 (AKT3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCAG-FLAG-puro-PTPIP51(1-150_175-470)

Plasmid

#170537

Purpose

Expresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfection

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Tags

FLAG tag

Expression

Mammalian

Mutation

deleted amino acids 151-175

Promoter

CAG promoter

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only
pQE32-pr-mEosFP (mEosFP-V69T)

Plasmid

#99213

Purpose

Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEosFP

Expression

Bacterial

Mutation

monomeric variant with mutation V69T for primed c…

Promoter

T5

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {hCAR}off-{ChETA-mRuby2}on-W3SL

Plasmid

#111391

Purpose

AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

hCAR (CXADR Human)
ChETA-mRuby2

Use

AAV

Tags

Myc and mRuby2

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e

Plasmid

#90010

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Cdc25C WT
NBirA*-14-3-3e

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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L40C-CRISPR.EFS.dTomato

Plasmid

#89392

Purpose

Lentiviral CRISPR-Cas9 delivery for sgRNA (hU6), dTomato coexpression, EFS Promoter driven

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P33

Plasmid

#120018

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with FLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

FLAG

Use

Pcr template

Tags

FLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z15

Plasmid

#120058

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with miRFP670

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

miRFP670

Use

Pcr template

Tags

miRFP670

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z02

Plasmid

#120045

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TagBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TagBFP

Use

Pcr template

Tags

TagBFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMINTC3GH (Kan)

Plasmid

#110076

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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SpyCas9(WT)

Plasmid

#101044

Purpose

Express Streptococcus pyogenes Cas9

Depositor

Zhiwei Huang

Article

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

SpyCas9

Tags

GST

Expression

Bacterial

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pETM6-RgTALsyn-HpaBC

Plasmid

#100949

Purpose

Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Inserts

RgTALsyn
HpaB
HpaC

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-Cys-ELP(120nm)-LPETGG

Plasmid

#90472

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

Cys-ELP(120nm)-LPETGG

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-GGG-ELP(120nm)-Cys

Plasmid

#91572

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-ELP(120nm)-Cys

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pet16b ALIX Bro1 (1-359)

Plasmid

#80641

Purpose

Residues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

ALIX Bro1, residues 1-359 (PDCD6IP Human)

Tags

His Tag

Expression

Bacterial

Mutation

Residues 1-359

Promoter

T7

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pWKS1583

Plasmid

#70188

Purpose

Modular vector for secreted amylase reporter expression

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

Insert

amyEopt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pGKO2-Gateway

Plasmid

#63618

Purpose

a binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants. Uses Gateway cloning.

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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