We narrowed to 9,668 results for: bli

1. pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6

   Plasmid

   #159913

   Purpose

   Mutagenesis of Kcnj6

   Depositor

   Larry Zweifel

   Insert

   Kcnj6 (Kcnj6 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. 4IA9

   Plasmid

   #51320

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   WDR5 (WDR5 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Truncation: See Comments

   Promoter

   T7

   Available Since

   Feb. 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. HECW2

   Plasmid

   #32875

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HECW2 (HECW2 Human)
   

   Tags

   His tag (with TEV cleavage)

   Expression

   Bacterial

   Available Since

   Jan. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pBACgus_Sumostar/LRRK2 1867-2161_C2024S/C2025S

   Plasmid

   #40394

   Depositor

   Michael J Fox Foundation MJFF

   Inserts

   SUMO
   LRRK2 (LRRK2 Human)
   

   Use

   Baculovirus

   Tags

   6xHis and HisTEV

   Mutation

   aa1867-2161,C2024S/C2025S

   Promoter

   polH

   Available Since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. 4PZI

   Plasmid

   #61955

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   MLL4:JMC054-D08:C226821 (KMT2B Human)
   

   Tags

   6xHis and MHHHHHHSSGRENLYFQG

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available Since

   March 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. Hs.KRAS4b C185S

   Plasmid

   #83139

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), C185S mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   C185S

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PIWIL2

   Plasmid

   #32042

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PIWIL2 (PIWIL2 Human)
   

   Tags

   His (with TEV cleavage)

   Expression

   Bacterial

   Mutation

   PAZ domain only (amino acids 386-533)

   Available Since

   Jan. 3, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

8. EPB41

   Plasmid

   #32040

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   EPB41 (EPB41 Human)
   

   Tags

   His (with TEV cleavage)

   Expression

   Bacterial

   Mutation

   FERM domain only (amino acids 1-279)

   Available Since

   Jan. 13, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

9. HSD17B8

   Plasmid

   #38853

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   HSD17B8 (Hsd17b8 Human)
   

   Tags

   Tev His Flag

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. GHRHR

    Plasmid

    #39173

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GHRHR (GHRHR Human)
    

    Use

    Baculovirus expression

    Tags

    Signal-His6-TEV

    Mutation

    N20->Q mutation of putative glycosylsation sit…

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pTEI134

    Plasmid

    #126071

    Purpose

    Vector for expression of plastid targeted YFP1-10 (T203Y) in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Insert

    Chloroplastic FNR transit peptide
    

    Use

    Binary

    Tags

    YFP1-10

    Expression

    Plant

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Gli3ZF in AINGpCAGGS

    Plasmid

    #121972

    Purpose

    Gli3 zinc fingers

    Depositor

    James Briscoe

    Insert

    Gli3ZF (GLI3 Human)
    

    Use

    Chick electroporation

    Tags

    5xmyc

    Expression

    Mammalian

    Mutation

    N terminus and C terminus deletion only nt 1411-1…

    Available Since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Hs.KRAS4b Q61K

    Plasmid

    #83164

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61K mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61K

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAdTrack-mLIP

    Plasmid

    #49197

    Purpose

    adenoviral shuttle vector with mouse C/EBPbeta LIP fragment

    Depositor

    Jed Friedman

    Insert

    C/EBPbeta (LIP fragment) (Cebpb Mouse)
    

    Use

    Adenoviral

    Mutation

    only the LIP fragment

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

15. Myc-gamma2s-EGFP

    Plasmid

    #118040

    Purpose

    GABAA receptor expression (gamma2 short subunit) double tagged, near N terminus and at C-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG2 (Gabrg2 Rat)
    

    Tags

    EGFP and cMyc epitope EQKLISEEDL inserted between…

    Expression

    Mammalian

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5/FRT/TO/Flag-tGFP-Cryz

    Plasmid

    #110378

    Purpose

    Mammalian expression of turboGFP-CRYZ with FLAG tag, Flp-In T-Rex system

    Depositor

    Sandra Martha Gomes Dias

    Insert

    crystallin zeta (CRYZ Human)
    

    Tags

    FLAG and tGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDNR-hSox2

    Plasmid

    #49389

    Purpose

    donor vector for cloning human Sox2 with 6xHN tag

    Depositor

    Randall McKinnon

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Cre/Lox; Donor vector

    Tags

    6xHN (6 repeating His-Asn subunits)

    Mutation

    TGA (stop) to CCA (Pro) at nt 1027 with respect t…

    Promoter

    none

    Available Since

    Nov. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

18. Mouse Alpha2 delta-2 mutMIDAS HA pMT2

    Plasmid

    #58733

    Purpose

    expression of HA tagged mouse Alpha2 delta-2 with MIDAS site mutations in mammalian cells

    Depositor

    Annette Dolphin

    Insert

    alpha2 delta-2 (Cacna2d2 Mouse)
    

    Expression

    Mammalian

    Mutation

    MIDAS site mutations (D300VSGS to AVAGA)

    Promoter

    SV40

    Available Since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK571

    Plasmid

    #75379

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Gene synthesis

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glycine-209 to aspartate and synthetic ge…

    Promoter

    n/a

    Available Since

    Aug. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBACgus N-His/GST/LRRK2 1867-2176

    Plasmid

    #40406

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    GST-Tev and HisTev

    Mutation

    WT aa1867-2176

    Promoter

    polH

    Available Since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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