We narrowed to 16,664 results for: yeast expression

1. pUDC175

   Plasmid

   #103019

   Purpose

   S. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoter

   Depositor

   Jean-Marc Daran

   Article

   Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

   Insert

   Fncpf1
   

   Tags

   3HA and NLS

   Expression

   Yeast

   Mutation

   human codon optimized

   Promoter

   TEF1

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJZC638

   Plasmid

   #62283

   Purpose

   Expresses MCP-VP64 and dCas9 in Yeast cells

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   MCP-VP64
   dCas9
   

   Tags

   VP64

   Expression

   Yeast

   Mutation

   Nuclease activity has been deactivated by mutatio…

   Promoter

   pAdh and pGal10

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. p415TEF cyto roGFP(iL)-Grx1(human)

   Plasmid

   #65002

   Purpose

   Yeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized roGFP; human Grx1

   Depositor

   Tobias Dick

   Article

   Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Tags

   roGFP(iL)

   Expression

   Yeast

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. KM-CashGem-Xyl2-URA3-LINEAR

   Plasmid

   #174838

   Purpose

   pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   Codon optimized Cas9:hGem
   

   Use

   CRISPR and Synthetic Biology

   Tags

   hGeminin tag

   Expression

   Bacterial and Yeast

   Promoter

   TEF1p

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pL90-Nat-2xHO

   Plasmid

   #139069

   Purpose

   CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

   Depositor

   Gianni Liti

   Article

   Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

   Insert

   Two guide RNAs targeting the HO endonuclease
   

   Expression

   Bacterial and Yeast

   Promoter

   TDH3

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGBT9-Hook2

   Plasmid

   #198524

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   Hook2 (HOOK2 Human)
   

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   His488Gln substitution

   Promoter

   ADH1

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUDR211

   Plasmid

   #113870

   Purpose

   expression of Cas9 programming sgRNA3 and sgRNA4 targetting HXT8 and HXT1 respectively

   Depositor

   Jean-Marc Daran, Jack Pronk

   Article

   Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

   Insert

   sgRNA3-HXT8 / sgRNA4-HXT14
   

   Expression

   Yeast

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUDP010

   Plasmid

   #101166

   Purpose

   E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Insert

   HH-gRNA-HDV targetting SeILV6 in S. pastorianus
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYO286

   Plasmid

   #235730

   Purpose

   Protein expression of yeast VPS15 (1-981)-ZZ in yeast

   Depositor

   Roger Williams

   Article

   Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

   Insert

   VPS15
   

   Tags

   3xTEV-ZZ

   Expression

   Yeast

   Mutation

   aa 1-981

   Available Since

   Oct. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYI2206

    Plasmid

    #228348

    Purpose

    Constitutive rPhlTA mutant expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    phlTA mutant
    

    Use

    Synthetic Biology

    Tags

    Three copies of VP16 (VP48)-Nuclear localization …

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K

    Promoter

    KpGAPDH promoter

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT1026

    Plasmid

    #228299

    Purpose

    Constitutive expression of PhlTA mutant with weak promoter and terminator

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    phlTA mutant
    

    Use

    Synthetic Biology

    Tags

    Three copies of VP16 (VP48)-Nuclear localization …

    Expression

    Yeast

    Mutation

    E41G

    Promoter

    KpYPT1 promoter

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT478

    Plasmid

    #228286

    Purpose

    Constitutive mutant rPhlTA expression with strong promoter

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    rphlTA mutant
    

    Use

    Synthetic Biology

    Tags

    Three copies of VP16 (VP48)-Nuclear localization …

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K

    Promoter

    KpGAPDH promoter

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETcon-pGAL1-Aga2-Htt(25Q)-c_myc

    Plasmid

    #225228

    Purpose

    Yeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.

    Depositor

    Urartu Seker

    Article

    Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

    Inserts

    Aga2
    Htt (25Q)
    

    Tags

    HA Tag and c-myc Tag

    Expression

    Yeast

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETcon-pGAL1-Aga2-Htt(46Q)-c_myc

    Plasmid

    #225229

    Purpose

    Yeast optimized human Htt (46Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (46Q) in yeast surface display.

    Depositor

    Urartu Seker

    Article

    Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

    Inserts

    Aga2
    Htt (46Q)
    

    Tags

    HA Tag and c-myc Tag

    Expression

    Yeast

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. BS-ZJLEU-PTEF1-GFP

    Plasmid

    #207018

    Purpose

    Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    GFP
    

    Expression

    Yeast

    Promoter

    pTEF1

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP

    Plasmid

    #207024

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    D384A Y387A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP

    Plasmid

    #207023

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    W371A I375A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. BS-ZJLEU-PTEF1-HFL1-Y387A-GFP

    Plasmid

    #207022

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Y387A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. BS-ZJLEU-PTEF1-HFL1-D384A-GFP

    Plasmid

    #207021

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    D384A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BS-ZJLEU-PTEF1-HFL1-I375A-GFP

    Plasmid

    #207020

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    I375A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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