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pAAV-EF1α1.1-FLEX-rc [ChromeQ-GFP]

Plasmid

#153542

Purpose

AAV-mediated expression of ChromeQ-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeQ-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

EF1α1.1

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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908I

Plasmid

#160241

Purpose

VanO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-SV40-Ubiquitin-Renilla-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

Van Operator (2x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter

Tags

T2A GFP

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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CJ50 (cg11094 utr mut)

Plasmid

#17778

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

cg11094 utr fragment mut

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

mutant version

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
CJ58 (cg6551 utr mut)

Plasmid

#17784

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

cg6551 utr fragment mut

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

mutant version

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pIND21-puro_SOX10-OLIG2-3xFLAG

Plasmid

#250321

Purpose

Tetracycline-inducible expression of SOX10-OLIG2 as polyprotein linked by self cleavage sites P2A. rtTA (Tet-ON) is driven the EF1alpha promoter.

Depositor

Michael Wehr

Article

Raabe et al Cells. 2022 Jan 11;11(2):241. doi: 10.33

Insert

SOX10-P2A-OLIG2 (SOX10, OLIG2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE2

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV/EF1a::DIO-iLMO2

Plasmid

#129482

Purpose

AAV2 transfer plasmid for Cre-dependent expression of iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical

Depositor

Robert Gross

Article

Jaiswal et al J Neurosci Res. 2017 Aug 3. doi: 10.1002

Insert

iLMO2

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_H0-ABD+

Plasmid

#234552

Purpose

CTNNA1 enhanced-actin-binding domain mutations

Depositor

Cara Gottardi

Article

Quinn et al Mol Biol Cell. 2024 May 1;35(5):ar65. do

Insert

mEGFP:hCTNNA1_H0-ABD+ (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

R666G, A667S, I668G, M669S

Promoter

CMV

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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EC.SurA.pTYB1.a21-281&390-428.M231R

Plasmid

#15958

Depositor

David McKay

Article

Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

Insert

Escherichia coli surA delta PPIase domain 2

Tags

intein

Expression

Bacterial

Mutation

residues 282-389 deleted; M231R mutation

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pLV-mEGFP-hCTNNA1_M-Open-DDD

Plasmid

#234561

Purpose

CTNNA1 M2-M3 salt-bridge disrupting mutant

Depositor

Cara Gottardi

Article

Quinn et al Mol Biol Cell. 2024 May 1;35(5):ar65. do

Insert

mEGFP:hCTNNA1_M-Open-DDD (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

D392A, D500A, D503A

Promoter

CMV

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_M-Open-KRR

Plasmid

#234562

Purpose

CTNNA1 M2-M3 salt-bridge disrupting mutant

Depositor

Cara Gottardi

Article

Quinn et al Mol Biol Cell. 2024 May 1;35(5):ar65. do

Insert

mEGFP:hCTNNA1_M-Open-KRR (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

K525A, R548A, R551A

Promoter

CMV

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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BLaER1 pgRNA CRISPR library

Pooled Library

#183825

Purpose

Paired gRNA library targeting genes related to B-cell to macrophage transdifferentiation.

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only
TB204 △hisD

Bacterial Strain

#229548

Purpose

Fluorescently labelled (sfGFP) E. coli, auxotrophic for histidine

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △metB

Bacterial Strain

#229549

Purpose

Fluorescently labelled (sfGFP) E. coli, auxotrophic for methionine

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △hisD proB74

Bacterial Strain

#229550

Purpose

Fluorescently labelled (sfGFP) E. coli, auxotrophic for histidine, proline overproducer

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △metB proB74

Bacterial Strain

#229551

Purpose

Fluorescently labelled (sfGFP) E. coli, auxotrophic for methionine, proline overproducer

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
TB205 △proC △trpR

Bacterial Strain

#229546

Purpose

Fluorescently labelled (mCherry) E. coli, auxotrophic for proline, tryptophan overproducer

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △trpC proB74

Bacterial Strain

#229547

Purpose

Fluorescently labelled (sfGFP) E. coli, auxotrophic for tryptophan, proline overproducer

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
TB205 △proC △hisL

Bacterial Strain

#229552

Purpose

Fluorescently labelled (mCherry) E. coli, auxotrophic for proline, histidine overproducer

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
pDEST-DB

Plasmid

#215482

Purpose

Yeast expression plasmid

Depositor

David E Hill

Article

Luck et al Nature. 2020 Apr;580(7803):402-408. doi:

Type

Empty backbone

Expression

Yeast

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pSO5

Plasmid

#106364

Purpose

A vector backbone, including mKate, to be used to make constructs to be inserted as a single copy at a site on Chr I.

Depositor

Jonathan Ewbank

Article

Lee et al Virulence. 2018 Dec 31;9(1):648-658. doi

Type

Empty backbone

Expression

Worm

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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