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YCplac111-KOG1 (pAN58)

Plasmid

#39209

Depositor

Michael Hall

Article

Loewith et al Mol Cell. 2002 Sep;10(3):457-68.

Insert

KOG1 (KOG1 Budding Yeast)

Tags

myc

Expression

Yeast

Mutation

L1512P

Promoter

KOG1

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only

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HL 3152 (=HL 716 + pHL 1056)

Plasmid

#30048

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pCon:MicC, pLlacO-1:rpoS::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
BacPAK Flag-NFRKB 1-465

Plasmid

#19788

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

NFRKB (NFRKB Human)

Tags

Flag and His

Expression

Insect

Mutation

only contains amino acids 1-465

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
HL 2817 (=HL 716 + pHL 720)

Plasmid

#30031

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:mCherry, pLlacO-1:sodB::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
HL 4120 (=HL 716 + pHL 1370)

Plasmid

#30094

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:DsrA, pCon:T7(st3) (900bps), pLlacO-1:rpoS::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only
HL 4180 (=HL 2752 + pHL 1358)

Plasmid

#30099

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLlacO-1:hfq, pLtetO-1m9:sodB::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only
pENTR-fishRab8B[Q67L]

Plasmid

#24908

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

Rab8B (rab8b Zebrafish)

Use

Gateway entry vector

Mutation

Changed Glutamine 67 to Leucine

Available Since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
Dom34 K82A

Plasmid

#19112

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

C tag

Expression

Bacterial

Mutation

K82A

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
SSO0083

Plasmid

#19097

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

PelA (Cell division protein pelota homolog) (Orf c04039 protein)

Tags

N tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO V5 DNAJB3

Plasmid

#19525

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 3 (DNAJB3 Mouse)

Tags

V5

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pLV hU6-gRNA hUbC-dSpCas9-2xVP64-2A-PuroR

Plasmid

#253229

Purpose

Expresses gRNA and VP64-dSpCas9-VP64-2A-PuroR from lentiviral vector

Depositor

Charles Gersbach

Article

Reisman et al bioRxiv 2025.10.11.681828

Insert

Humanized VP64-dSpCas9-VP64-2A-PuroR

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pLV hU6-gRNA hUbC-dSpCas9-2xVP64-2A-Thy1.1

Plasmid

#253230

Purpose

Expresses gRNA and VP64-dSpCas9-VP64-2A-Thy1.1 from lentiviral vector

Depositor

Charles Gersbach

Article

Reisman et al bioRxiv 2025.10.11.681828

Insert

Humanized VP64-dSpCas9-VP64-2A-Thy1.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV_aCatTS_indicator

Plasmid

#252386

Purpose

Non-FRET aCatenin tension indicator. cpEGFP with elastic linker is inserted at the hinge with C-term mCherry.

Depositor

Maretoshi Hirai

Article

Fujiwara et al Commun Biol. 2026 Feb 19. doi: 10.1038/s

Insert

aCatenin tension indicator

Tags

mCherry

Expression

Mammalian

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV_aActTS_indicator

Plasmid

#252385

Purpose

Non-FRET aActinin tension indicator. cpEGFP with elastic linker is inserted at the hinge with C-term mCherry.

Depositor

Maretoshi Hirai

Article

Fujiwara et al Commun Biol. 2026 Feb 19. doi: 10.1038/s

Insert

aActinin tension indicator

Tags

mCherry

Expression

Mammalian

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_J23102_R1_LuxR

Plasmid

#250887

Purpose

LuxR regulator protein expressed under BBa_J23102 promoter; part of BNeu AS4, AS8, AS9 and AS14; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

LuxR

Use

Synthetic Biology

Promoter

BBa_J23102

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pVG298

Plasmid

#227762

Purpose

Expresses a version of light-inducible transcription factor EL222 (Thr83Ala) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Thr83Ala mutant

Promoter

PGK1pr

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLux_R1_CI*

Plasmid

#250883

Purpose

Expresses frame-shifted cI under the control of PLux promoter; part of BNeuAS2 and BNeuAS3; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

Mutated cI

Use

Synthetic Biology

Mutation

cI* = Frame-shifted cI protein

Promoter

Plux

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a IRES mNeonGreen-P2A-Puro

Plasmid

#248030

Purpose

Backbone that constitutively expresses mNeonGreen and a puromycin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1467_pTet-ECOR31Ham_hamB_walkerB_Mut

Plasmid

#223367

Purpose

Expression of ECOR31 Hachiman mutant (HamB D431A) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman mutant (HamB D431A)

Expression

Bacterial

Mutation

HamB D431A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1469_pTet-ECOR31Ham_hamA_E138A_K140A_Mut

Plasmid

#223368

Purpose

Expression of ECOR31 Hachiman mutant (HamA E138A, K140A) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman mutant (HamA E138A, K140A)

Expression

Bacterial

Mutation

HamA E138A, K140A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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