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pNL3.2.CMV.5UTR-TH.NLuc-PEST.Hygro

Plasmid

#209953

Purpose

In mammalian cells expresses NanoLuc mRNA with the TH in the 5'-UTR. Also expresses a hygromycin resistance marker.

Depositor

Kevin Weeks

Article

Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38

Inserts

NanoLuc
Hygromycin resistance marker

Expression

Mammalian

Promoter

CMV and SV40

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_003

Plasmid

#210760

Purpose

SC_Hygromycin phosphotransferase

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

Hygromycin phosphotransferase

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_AaNHis_RbcL

Plasmid

#229517

Purpose

Expresses Anthoceros agrestis Rubisco large (N terminus hexahistidine)

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Insert

Rubisco large subunit

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-AzaQ

Plasmid

#224429

Purpose

Express AzaQ in E. coli

Depositor

Emily Balskus

Article

Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

Insert

azaQ

Tags

His6

Expression

Bacterial

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pJJB170

Plasmid

#218591

Purpose

Express engineered TFs Adr1c(S230A), Pip2c, and Oaf1c

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Adr1

Expression

Yeast

Mutation

Adr1(S230A), Pip2(1-168 + 2,497-2,988), Oaf1(1-30…

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1340

Plasmid

#218593

Purpose

Express a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22. Also overexpresses Pex11

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex22

Expression

Yeast

Mutation

Pex22(1-36)

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-AzaM

Plasmid

#224428

Purpose

Express AzaM in E. coli

Depositor

Emily Balskus

Article

Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

Insert

azaM

Tags

His6

Expression

Bacterial

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-AzaC

Plasmid

#224424

Purpose

Express AzaC in E. coli

Depositor

Emily Balskus

Article

Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

Insert

azaC

Tags

His6

Expression

Bacterial

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-AzaD

Plasmid

#224425

Purpose

Express AzaD in E. coli

Depositor

Emily Balskus

Article

Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

Insert

azaD

Tags

His6

Expression

Bacterial

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-AzaB

Plasmid

#224423

Purpose

Express AzaB in E. coli

Depositor

Emily Balskus

Article

Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

Insert

azaB

Tags

His6

Expression

Bacterial

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-AzaG

Plasmid

#224427

Purpose

Express AzaG in E. coli

Depositor

Emily Balskus

Article

Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

Insert

azaG

Tags

His6

Expression

Bacterial

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-AzaE

Plasmid

#224426

Purpose

Express AzaE in E. coli

Depositor

Emily Balskus

Article

Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

Insert

azaE

Tags

His6

Expression

Bacterial

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWR95(URA3)

Plasmid

#203917

Purpose

BLINCAR plasmid for gene deletion, URA3 target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

URA3 upstream homology
URA3 downstream homology

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMS161

Plasmid

#215683

Purpose

Guide only plasmid targeting chrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTCCAGCGGCAGATCGGCGG

Use

CRISPR

Expression

Worm

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMS160

Plasmid

#215682

Purpose

Guide only plasmid targeting chrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTTTGAGTAGAGCACTCAGG

Use

CRISPR

Expression

Worm

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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IL1_1

Plasmid

#207623

Purpose

Low copy plasmid expressing mTurquoise2 under the TDH3p promoter

Depositor

Yvonne Nygård

Article

Mormino et al Microb Cell Fact. 2022 Oct 15;21(1):214.

Insert

mTurquoise2

Expression

Yeast

Promoter

ScTDH3

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pWR95

Plasmid

#203916

Purpose

BLINCAR plasmid for gene deletion, no genomic target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

modified MCS

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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Atg32 [200-341] 1B

Plasmid

#190431

Purpose

Recombinant expression plasmid of S. cerevisiae Atg32 amino acids 200 - 341 in E. coli.

Depositor

Michael Ragusa

Article

Xia et al Autophagy. 2018;14(9):1620-1628. doi: 10

Insert

Atg32

Tags

6xHis-TEV

Expression

Bacterial

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-Mss4-Kina

Plasmid

#202724

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:S. cerevisiae Mss4(377-756) (MSS4 Budding Yeast)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-FLPe-9

Plasmid

#197724

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

FLPe recombinase (tomato optimized)

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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