...things!   Myc1-9E10 and the Myc-tag today Since Munro and Pelham introduced the Myc tag to the world...commonly used Myc-tag. Who first started using it in protein tagging experiments? Why Myc? When did the...probe the functions of the human c-Myc gene (Evan, et al. 1985). Myc was one of the earliest identified...when Evan’s team generated their Myc antibodies. At first, anti-c-Myc [9E10] was just one of several new...  Find recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the myc-tag  ...C-terminal antibodies was Myc1-9E10 (aka anti-c-Myc [9E10]). They noticed that Myc1-9E10 and the other C-terminal...anti-Myc [9E10] as a recombinant antibody. Researchers first sequenced the variable domains of Myc1-9E10...

...proto-oncogene c-Myc. Since the generation of the first anti-c-Myc [9E10] (Even, et al., 1985), the Myc tag has...light-gated potassium channelrhodopsins Myc-tag antibody anti-c-Myc [9E10] now available! Improved voltage...doi.org/10.1101/2021.09.17.460684.)   Myc-tag antibody anti-c-Myc [9E10] now available! by: Ashley Waldron...to guess that the Myc-tag is one of them (and not just because of the title!) The Myc-tag is a string of... for ‘Myc tag’ in the Addgene catalog pulled up over 13,000 plasmids! Our recombinant anti-c-Myc [9E10... of Addgene’s western blot for the myc-tagged protein GFP-smFP-myc expressed from Plasmid 98926. See the... the Anti-c-Myc [9E10] page for more details. Image credit: Addgene.   Find anti-c-Myc antibody here...

...monoclonal (M2) as well as a chimera (rabbit). Myc The Myc tag is a 10 amino acid peptide (EQKLISEEDL) derived...negatively charged and 1 positively charged amino acids. Myc tags are frequently used for western blot, flow cytometry... However, protein purification is not ideal with Myc, as low pH conditions are required during the purification...affect protein function. Fun fact: The antibody to Myc was developed in 1985 and is still the most prevalent...Addgene has you covered with monoclonal antibodies to Myc for all of these species. HA The HA tag is a 9 amino...availability Plasmids 101: Protein Tags Antibodies: Of Myc and Men    ...

...either with a labeled anti- HA or -MYC antibody or with an anti-HA or -MYC primary and a labeled secondary... Spaghetti Monster: HA (H) or Myc (M) versions Labeled anti-HA or -Myc antibody Staining cultured cells... are highly antigenic tags that have 10 HA or 10 MYC tags built into a GFP scaffold. Some GFP nanobodies...RANbody detection with a directly labeled anti-HA or -MYC antibody is less sensitive than HRP RANbody detection...sections When detected with a labeled anti-HA or -MYC antibody, approximately as sensitive as detecting...secondary antibody staining HA Tagged GFP, RFP, H2A2B Myc Tagged RFP, H2A2B Chicken IgY (Y) Labeled Anti-Chicken...

...enhances iPSC generation as does replacing C-MYC with L-MYC [10, 11]. While the mechanisms underlying the...addition of an shRNA for P53 and replacing C-MYC with L-MYC in the episomal reprogramming cocktail by Okita...reprogramming factors- OCT3/4, SOX2, KLF4, and C-MYC [1]. For the derivation of clinical grade hiPSCs,...that encoded six factors - OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen (SV40LT). The method...pCXLE-hSK 27078 SOX2 and KLF4 pCXLE-hUL 27080 L-MYC and LIN28 pCXLE-hOCT3/4-shp53-F 27077 OCT3/4 and...direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci U S A, 2010. 107(32): p. 14152...

...you encounter mycoplasma contamination. Causes of mycoplasma contamination The mycoplasmas enter the cell...antibacterial drugs like penicillin and streptomycin. Mycoplasma can pass through filtration methods because... growth of mycoplasmas. The serum is nutrient-rich and also the best source for mycoplasma proliferation...suppress the growth of mycoplasma but cannot eradicate it completely. When mycoplasmas are seen in the culture...highly expensive). Detecting mycoplasma contamination Detection of mycoplasma by naked eyes or optical microscope...most of the mycoplasma species, apart from a few of them. Another method used for mycoplasma detection ...infecting mycoplasmas are used. A broad range of primers encompasses almost all the infecting mycoplasma. The...

...genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can...selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide... can be applied for identification of essential MYC binding sites in a wide range of human cells.  A new...’s lab previously developed such a system in Streptomyces, a prokaryotic genus capable of producing a ...

...expression cloning of novel oncogenes beyond ras and myc.  For this purpose we needed vectors that could stably...from tumors.  In addition, to study the biology of myc in detail via collaborations with labs headed by ...including one of the first to confer resistance to puromycin, vastly decreasing the cost of stable selection...selection relative to the then heavily used neomycinR/G418 tandem selection) we had to name them. In honor of...

...epitope tags? Short epitope tags like FLAG, HA, and Myc can adopt a number of different conformations and... fusions to inntags or traditional FLAG, HA, and Myc tags. Impressively, IT6-GFP did not reduce yeast ...

...protocol starts out with anti-Myc antibodies bound to an ELISA plate to capture Myc-tagged sybodies. Then, the...the expression vector pSb-Init, which attaches a Myc-tag and a His6-tag to the end of each sybody candidate...

...fluorescent proteins (GFP and YFP) and protein tags (c-MYC and HA). We have also created the Monoclonal Antibody...

...to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have been the workhorses of the affinity tag...step purification with amylose, but wicked huge Myc EQKLISEEDL  1.2 Affinity Frequently used for western...

... viral particles that express oncogenes, such as Myc. Viral vectors also have the largest genomic footprint...transgenes is a concern as well, and reactivation of Myc or other oncogenes after differentiation has been...including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check out these plasmids if you’re interested...

...state using a cocktail of factors (Oct3/4, Sox2, c-Myc, and Klf4) that are known to maintain pluripotency...Lentivirus Human Expression of human Oct4, Sox2, Klf4, Myc, and Brd3R from five separate lentiviral plasmids...Doxycycline-inducible expression of human Oct4, Sox2, Klf4, and c-Myc from four separate lentiviral plasmids A drug-inducible... the expression of human Oct4, Klf4, Sox2, and c-Myc Human Induced Pluripotent Stem Cells Produced Under...vector for the expression of human Oct4, Klf4, c-Myc, and Sox2 Integrative Analyses of Human Reprogramming...Lentivirus Human Expression of human Klf4, Oct4, c-Myc, and Sox2 as VP-16 transcriptional activating fusions...polycistronic expression of human Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53 for single plasmid reprogramming...

...different types of tags, including epitope tags (FLAG, Myc, etc.) and fluorescent protein tags (GFP, mCherry...

...pCMVTNT PINK1 N-myc PINK1 Myc CMV Parkinson's Mark Cookson 13314 pCMVTNT PINK1 C-myc PINK1 Myc CMV Parkinson's...Ultra-Exon1Q23-Myc-A HTT Myc UbC Huntington's Baoji Xu 110487 Ultra-Exon1Q145-Myc-A HTT Myc UbC Huntington's...pUltra-Exon1Q23-Myc-B HTT Myc UbC Huntington's Baoji Xu 110489 pUltra-Exon1Q145-Myc-B HTT Myc UbC Huntington's...Strep-Lrrk2-Myc LRRK2 Myc, Strep CMV Parkinson's Maik Hintze 161583 pPuro3.1(+)_Strep-Lrrk2-Myc LRRK2 Myc, Strep...LRRK2-WT LRRK2 His, Myc CMV Parkinson's Ted Dawson 17612 pRK5-Myc-Parkin PRKN Myc CMV Parkinson's Ted ... pGW1-Myc-DJ1-WT PARK7 V5 CMV Parkinson's Mark Cookson 29349 pGW1-Myc-DJ1-L166P/K130R PARK7 Myc CMV Parkinson's...D1994S)-Myc LRRK2 Myc, Strep CMV Parkinson's Maik Hintze 161585 pPuro3.1(+)_Strep-Lrrk2(G2019S)-Myc LRRK2...

...pTag-RFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc Early endosomes...pTag-RFP-C-h-Rab11a-c-Myc Recycling endosomes Rab11a TagRFP James Johnson 79805 pTag-BFP-C-h-Rab11a-c-Myc Recycling...pTag-RFP-C-h-Rab4a-c-Myc Recycling endosomes Rab4a TagRFP James Johnson 79799 pTag-BFP-C-h-Rab4a-c-Myc Recycling ... NLS mKalama1 Robert Campbell 73205 pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from Mak16p protein)...p63 mTurquoise2 Dorus Gadella 73206 pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian) PDGFR-derived...SKL mNeonGreen Dorus Gadella 73210 pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL Peroxisome SKL tripeptide, peroxisome...Reticulum Sec61 BFP Gia Voeltz 73209 pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL Endoplasmic Reticulum KDEL dL5...

...and C-terminal tags (StrepII, 3xFLAG, 6xHIS, MBP, Myc). These constructs include proteins like nonstructural...

...C-terminal 3xHA tag for yeast expression Myc Epitope Tag pKMyc - N-terminal Myc tag for mammalian...expression 6xHis Epitope Tag pEZYmyc-His - C-terminal Myc-His tag for mammalian expression...SP6 promoter pCS2 mt-GFP - Create myc-tag, GFP-tag fusions for transcription pCSDest - ... Protein Guide NLS Nuclear Localization pENTR4-myc-nuc - pEntry vector that adds a C-...Organism Representative Empty Backbones Neomycin (G418) Mammalian pBABE-neo - Retroviral ...destination vector for gene expression Puromycin Mammalian pBABE-puro - Retroviral ...destination vector for gene expression Hygromycin Mammalian pBABE-hygro - Retroviral ...

...four reprogramming factors- OCT4, SOX2, KLF4, and C-MYC - that induce a differentiated somatic cell to revert...additional pluripotency stage) Less Safety Less (C-MYC, a known oncogene is one of the reprogramming factors...