...things!   Myc1-9E10 and the Myc-tag today Since Munro and Pelham introduced the Myc tag to the world...commonly used Myc-tag. Who first started using it in protein tagging experiments? Why Myc? When did the...probe the functions of the human c-Myc gene (Evan, et al. 1985). Myc was one of the earliest identified...when Evan’s team generated their Myc antibodies. At first, anti-c-Myc [9E10] was just one of several new...  Find recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the myc-tag  ...C-terminal antibodies was Myc1-9E10 (aka anti-c-Myc [9E10]). They noticed that Myc1-9E10 and the other C-terminal...anti-Myc [9E10] as a recombinant antibody. Researchers first sequenced the variable domains of Myc1-9E10...

...proto-oncogene c-Myc. Since the generation of the first anti-c-Myc [9E10] (Even, et al., 1985), the Myc tag has...light-gated potassium channelrhodopsins Myc-tag antibody anti-c-Myc [9E10] now available! Improved voltage...doi.org/10.1101/2021.09.17.460684.)   Myc-tag antibody anti-c-Myc [9E10] now available! by: Ashley Waldron...to guess that the Myc-tag is one of them (and not just because of the title!) The Myc-tag is a string of... for ‘Myc tag’ in the Addgene catalog pulled up over 13,000 plasmids! Our recombinant anti-c-Myc [9E10... of Addgene’s western blot for the myc-tagged protein GFP-smFP-myc expressed from Plasmid 98926. See the... the Anti-c-Myc [9E10] page for more details. Image credit: Addgene.   Find anti-c-Myc antibody here...

...monoclonal (M2) as well as a chimera (rabbit). Myc The Myc tag is a 10 amino acid peptide (EQKLISEEDL) derived...negatively charged and 1 positively charged amino acids. Myc tags are frequently used for western blot, flow cytometry... However, protein purification is not ideal with Myc, as low pH conditions are required during the purification...affect protein function. Fun fact: The antibody to Myc was developed in 1985 and is still the most prevalent...Addgene has you covered with monoclonal antibodies to Myc for all of these species. HA The HA tag is a 9 amino...availability Plasmids 101: Protein Tags Antibodies: Of Myc and Men    ...

...either with a labeled anti- HA or -MYC antibody or with an anti-HA or -MYC primary and a labeled secondary... Spaghetti Monster: HA (H) or Myc (M) versions Labeled anti-HA or -Myc antibody Staining cultured cells... are highly antigenic tags that have 10 HA or 10 MYC tags built into a GFP scaffold. Some GFP nanobodies...RANbody detection with a directly labeled anti-HA or -MYC antibody is less sensitive than HRP RANbody detection...sections When detected with a labeled anti-HA or -MYC antibody, approximately as sensitive as detecting...secondary antibody staining HA Tagged GFP, RFP, H2A2B Myc Tagged RFP, H2A2B Chicken IgY (Y) Labeled Anti-Chicken...

...enhances iPSC generation as does replacing C-MYC with L-MYC [10, 11]. While the mechanisms underlying the...addition of an shRNA for P53 and replacing C-MYC with L-MYC in the episomal reprogramming cocktail by Okita...reprogramming factors- OCT3/4, SOX2, KLF4, and C-MYC [1]. For the derivation of clinical grade hiPSCs,...that encoded six factors - OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen (SV40LT). The method...pCXLE-hSK 27078 SOX2 and KLF4 pCXLE-hUL 27080 L-MYC and LIN28 pCXLE-hOCT3/4-shp53-F 27077 OCT3/4 and...direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci U S A, 2010. 107(32): p. 14152...

...you encounter mycoplasma contamination. Causes of mycoplasma contamination The mycoplasmas enter the cell...antibacterial drugs like penicillin and streptomycin. Mycoplasma can pass through filtration methods because... growth of mycoplasmas. The serum is nutrient-rich and also the best source for mycoplasma proliferation...suppress the growth of mycoplasma but cannot eradicate it completely. When mycoplasmas are seen in the culture...highly expensive). Detecting mycoplasma contamination Detection of mycoplasma by naked eyes or optical microscope...most of the mycoplasma species, apart from a few of them. Another method used for mycoplasma detection ...infecting mycoplasmas are used. A broad range of primers encompasses almost all the infecting mycoplasma. The...

...genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can...selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide... can be applied for identification of essential MYC binding sites in a wide range of human cells.  A new...’s lab previously developed such a system in Streptomyces, a prokaryotic genus capable of producing a ...

...expression cloning of novel oncogenes beyond ras and myc.  For this purpose we needed vectors that could stably...from tumors.  In addition, to study the biology of myc in detail via collaborations with labs headed by ...including one of the first to confer resistance to puromycin, vastly decreasing the cost of stable selection...selection relative to the then heavily used neomycinR/G418 tandem selection) we had to name them. In honor of...

...epitope tags? Short epitope tags like FLAG, HA, and Myc can adopt a number of different conformations and... fusions to inntags or traditional FLAG, HA, and Myc tags. Impressively, IT6-GFP did not reduce yeast ...

...protocol starts out with anti-Myc antibodies bound to an ELISA plate to capture Myc-tagged sybodies. Then, the...the expression vector pSb-Init, which attaches a Myc-tag and a His6-tag to the end of each sybody candidate...

...fluorescent proteins (GFP and YFP) and protein tags (c-MYC and HA). We have also created the Monoclonal Antibody...

...to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have been the workhorses of the affinity tag...step purification with amylose, but wicked huge Myc EQKLISEEDL  1.2 Affinity Frequently used for western...

... viral particles that express oncogenes, such as Myc. Viral vectors also have the largest genomic footprint...transgenes is a concern as well, and reactivation of Myc or other oncogenes after differentiation has been...including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check out these plasmids if you’re interested...

...different types of tags, including epitope tags (FLAG, Myc, etc.) and fluorescent protein tags (GFP, mCherry...

...and C-terminal tags (StrepII, 3xFLAG, 6xHIS, MBP, Myc). These constructs include proteins like nonstructural...

...four reprogramming factors- OCT4, SOX2, KLF4, and C-MYC - that induce a differentiated somatic cell to revert...additional pluripotency stage) Less Safety Less (C-MYC, a known oncogene is one of the reprogramming factors...

... transcription factors OCT3/4, SOX2, KLF4, and c-MYC is the established model for reprogramming somatic...article have been deposited with Addgene and include MYC- and FLAG-tagged TZAP constructs (wild-type and truncation... the B. subtilis Single Gene Deletion Library—Kanamycin and the CRISPRi Essential gene Knockdown library...regions (excluding start and stop codons) with kanamycin cassettes and were carefully designed to allow...are available at Addgene and include a variety of cMYC, KLF4, SOX2 and OCT4 DNA binding domain and transcription...

...Figure 1: A) Cell-penetrating Cas9, fused to HIV TAT, Myc and SV40 Nuclear Localization Signals, and GFP. B...

... T2A-TurboGFP-PEST), and small epitope tags (HA, Myc, Strep tag II, AviTag, HaloTag, SpyTag). The Foerstemann...YFP, Strep, TEV-V5) with either blasticidin or puromycin selection. Researchers at the Allen Institute ...

...of four embryonic genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated from mouse fibroblasts...