We narrowed to 19,050 results for: Tec

1. PatoL-OG241

   Plasmid

   #72841

   Purpose

   Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.

   Depositor

   Chris Barnes

   Article

   Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

   Insert

   PatoL
   

   Expression

   Bacterial

   Promoter

   PatoL

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB2909(XII-5 MarkerFree)

   Plasmid

   #73281

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. EK0493 SFFV-mCherry-SGc(GAPDH.s1)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191156

   Purpose

   Expression of a sensor for GAPDH 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:GAPDH.s1:EGFP-3xMS2
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

   Plasmid

   #181791

   Purpose

   Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
   

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcg 2.0-BFP-circ-arRNA backbone

   Plasmid

   #213965

   Purpose

   pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o

   Depositor

   Wensheng Wei

   Article

   Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-RIPK3[V458A][Q459A][V460A][G461A]-FLAG

   Plasmid

   #199328

   Purpose

   mutated RHIM motive (interaction inhibited)

   Depositor

   Adrian Press

   Article

   Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

   Insert

   Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed V458, Q459,V460 and G461 to alanine

   Available Since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2T-cmv-SpCas9-TadDE-BlastR

   Plasmid

   #193850

   Purpose

   Express TadDE in mammalian cells with BlastR selection, Tol2 integration

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadDE
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Rescue Septin 11 mRNA

   Plasmid

   #122022

   Purpose

   Rescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.

   Depositor

   Angel de Blas

   Article

   Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

   Insert

   SEPT11 (Sept11 Rat)
   

   Expression

   Mammalian

   Mutation

   Silent mutations are in 5 consecutive codons (AUU…

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. TS MS-Sensor

   Plasmid

   #199788

   Purpose

   Synthetic gene circuit

   Depositor

   Timothy Lu

   Article

   Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

   Insert

   TS MS-Sensor
   

   Use

   Synthetic Biology

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMW96

    Plasmid

    #178066

    Purpose

    Expression of His6-SUMO-TEV-GFP-PreScission-γ-TuNA in E. coli

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al Cell. 2020 Jan 9;180(1):165-175.e16. doi

    Insert

    CDK5RAP2 (CDK5RAP2 Human)
    

    Expression

    Bacterial

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. [pSK468] pLJC5 Flag-Depdc5

    Plasmid

    #109337

    Purpose

    Lenti-viral expression of Flag-tagged wildtype Depdc5

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAF0362 Ef1a-Cp36_attP-Puro-p2a-mCherry donor

    Plasmid

    #193469

    Purpose

    Cp36 Donor with promoter for mcherry and puro expression

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9-NG-CGBE1

    Plasmid

    #198537

    Purpose

    Expresses human codon-optimized SpCas9-NG-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-CGBE1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-CGBE1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTrc99S-ssDsbA-MBP-4xDQNAT

    Plasmid

    #128398

    Purpose

    Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Maltose binding protein
    

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Y342H- please see depositor comments

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EFS-fDIO-GRAB_eCB2.0

    Plasmid

    #164610

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in FLP positive cells

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Mutation

    S383T

    Promoter

    EFS

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. AA179

    Plasmid

    #215939

    Purpose

    Fragmid fragment: (guide cassette) short sequence with no known function

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Filler
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AA331

    Plasmid

    #215954

    Purpose

    Fragmid fragment: (guide cassette) guide expression

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v2 [Sa]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-DIO-GRAB_eCB2.0mut

    Plasmid

    #164607

    Purpose

    Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid non-responsive sensor GRAB_eCBmut
    

    Use

    AAV

    Mutation

    S383T, F177A

    Promoter

    hSyn

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. RS35075_pMQ30_KO_Kan

    Plasmid

    #185390

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS35075 upstream flanking homology region
    RR42_RS35075 downstream flanking homology region
    nptII kanamycin resistance marker
    

    Expression

    Bacterial

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX601-AAV-CMV-NLS-ISAba30-NLS-3xHA-bGHpA;U6-BbsI-reRNA

    Plasmid

    #205320

    Purpose

    All-in-one AAV vector expressing ISAba30 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISAba30 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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