Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 3,032 results for: 2-Oct

Showing: 1751 - 1800 of 3032 results

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pST39-Mega'-deltaBSK

Plasmid

#216952

Purpose

Mega' with knockout of GvpB, GvpS and GvpK

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Mega' with knockout of GvpB, GvpS and GvpK

Expression

Bacterial

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pST39-Mega'-deltaRNS

Plasmid

#216954

Purpose

Mega' with knockout of GvpR, GvpN and GvpS

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Mega' with knockout of GvpR, GvpN and GvpS

Expression

Bacterial

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pST39-Mega'-deltaBLK

Plasmid

#216950

Purpose

Mega' with knockout of GvpB, GvpL and GvpK

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Mega' with knockout of GvpB, GvpL and GvpK

Expression

Bacterial

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pST39-Mega'-deltaBLS

Plasmid

#216949

Purpose

Mega' with knockout of GvpB, GvpL and GvpS

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Mega' with knockout of GvpB, GvpL and GvpS

Expression

Bacterial

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pST39-Mega'-deltaBFG

Plasmid

#216948

Purpose

Mega' with knockout of GvpB, GvpF and GvpG

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Mega' with knockout of GvpB, GvpF and GvpG

Expression

Bacterial

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pST39-Mega'-deltaBRL

Plasmid

#216946

Purpose

Mega' with knockout of GvpB, GvpR and GvpL

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Mega' with knockout of GvpB, GvpR and GvpL

Expression

Bacterial

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-R-Lg

Plasmid

#216961

Purpose

Expression of GvpR-Linker-Large BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpR-Linker-Large BiT

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-Lg-F

Plasmid

#216966

Purpose

Expression of Large BiT-Linker-GvpF

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Large BiT-Linker-GvpF

Tags

FLAG-Split Nanoluciferase LargeBiT

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-F-Lg

Plasmid

#216965

Purpose

Expression of GvpF-Linker-Large BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpF-Linker-Large BiT

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-Lg-N

Plasmid

#216964

Purpose

Expression of Large BiT-Linker-GvpN

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Large BiT-Linker-GvpN

Tags

FLAG-Split Nanoluciferase LargeBiT

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-Lg-S

Plasmid

#216972

Purpose

Expression of Large BiT-Linker-GvpS

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Large BiT-Linker-GvpS

Tags

FLAG-Split Nanoluciferase LargeBiT

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-S-Lg

Plasmid

#216971

Purpose

Expression of GvpS-Linker-Large BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpS-Linker-Large BiT

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-Lg-G

Plasmid

#216968

Purpose

Expression of Large BiT-Linker-GvpG

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Large BiT-Linker-GvpG

Tags

FLAG-Split Nanoluciferase LargeBiT

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-G-Lg

Plasmid

#216967

Purpose

Expression of GvpG-Linker-Large BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpG-Linker-Large BiT

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-Lg-K

Plasmid

#216974

Purpose

Expression of Large BiT-Linker-GvpK

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Large BiT-Linker-GvpK

Tags

FLAG-Split Nanoluciferase LargeBiT

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-L-Lg

Plasmid

#216969

Purpose

Expression of GvpL-Linker-Large BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpL-Linker-Large BiT

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-Lg-T

Plasmid

#216978

Purpose

Expression of Large BiT-Linker-GvpT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Large BiT-Linker-GvpT

Tags

FLAG-Split Nanoluciferase LargeBiT

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-F-Sm

Plasmid

#216988

Purpose

Expression of GvpF-Linker-Small BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpF-Linker-Small BiT

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-Sm-R

Plasmid

#216985

Purpose

Expression of Small BiT-Linker-GvpR

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Small BiT-Linker-GvpR

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-K-Sm

Plasmid

#216996

Purpose

Expression of GvpK-Linker-Small BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpK-Linker-Small BiT

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-Sm-K

Plasmid

#216997

Purpose

Expression of Small BiT-Linker-GvpK

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Small BiT-Linker-GvpK

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-Lg-J

Plasmid

#216976

Purpose

Expression of Large BiT-Linker-GvpJ

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Large BiT-Linker-GvpJ

Tags

FLAG-Split Nanoluciferase LargeBiT

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-J-Lg

Plasmid

#216975

Purpose

Expression of GvpJ-Linker-Large BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpJ-Linker-Large BiT

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-Lg-L

Plasmid

#216970

Purpose

Expression of Large BiT-Linker-GvpL

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Large BiT-Linker-GvpL

Tags

FLAG-Split Nanoluciferase LargeBiT

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-K-Lg

Plasmid

#216973

Purpose

Expression of GvpK-Linker-Large BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpK-Linker-Large BiT

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-FRB-Lg

Plasmid

#216981

Purpose

Expression of FRB-Linker-Large BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

FRB-Linker-Large BiT

Tags

Split Nanoluciferase LargeBiT

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26b(modified)-Lg-U

Plasmid

#216980

Purpose

Expression of Large BiT-Linker-GvpU

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Large BiT-Linker-GvpU

Tags

FLAG-Split Nanoluciferase LargeBiT

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-SFFV_NKX2-1_Short_V5-GFP

Plasmid

#221500

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an SFFV promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Promoter

SFFV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-EF1a_NKX2-1_CdTag-HA

Plasmid

#221493

Purpose

NKX2-1 short isoform lentiviral overexpression of C-terminal dTag fusion

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-EF1a_NKX2-1_W208L

Plasmid

#221486

Purpose

NKX2-1 W208L DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

W208L

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-UBC_NKX2-1

Plasmid

#221495

Purpose

NKX2-1 short isoform lentiviral overexpression using UBC promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

UBC

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_NKX2-1_DelCTerm_1-223AA

Plasmid

#221476

Purpose

Gateway cloning of NKX2-1 C-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

Deletion of 224-371AA C-terminal domain

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_NKX2-1_DelNTerm_160-371AA

Plasmid

#221475

Purpose

Gateway cloning of NKX2-1 N-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

Deletion of 1-159AA N-terminal domain

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-EF1a_NKX2-1_DelNTerm_160-371AA

Plasmid

#221487

Purpose

NKX2-1 N-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 1-159AA N-terminal domain

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-EF1a_NKX2-1_V205F

Plasmid

#221485

Purpose

NKX2-1 V205F DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

V205F

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-hPGK_NKX2-1_Short_V5-GFP

Plasmid

#221498

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an hPGK promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Promoter

hPGK

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-EF1a_NKX2-1_DelCTerm_1-223AA

Plasmid

#221488

Purpose

NKX2-1 C-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 224-371AA C-terminal domain

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-SFFV_NKX2-1

Plasmid

#221496

Purpose

NKX2-1 short isoform lentiviral overexpression using SFFV promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

SFFV

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJC8

Plasmid

#170757

Purpose

Co-express in mammalian cells two target proteins C-terminally fused to V5 and FLAG tags respectively, using an internal ribosome entry site and two multi-cloning sites.

Depositor

Roberto Dominguez

Article

Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi

Type

Empty backbone

Tags

site 1- V5 tag and site 2- FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMP89b CvTA V124N

Plasmid

#141207

Purpose

Chromobacterium violaceum transaminase with enhances affinity for PLP. TEV cleaving site after the N-terminal Histag.

Depositor

Francesca Paradisi

Article

Roura Padrosa et al Front Bioeng Biotechnol. 2019 Oct 18;7:2

Insert

CvTA

Expression

Bacterial

Mutation

V124N

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1291 TDP43 RRM-GFP G368W

Plasmid

#107837

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G368W mutation in LLPS reporter

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1279 TDP43 RRM-GFP G348V

Plasmid

#107831

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G348V mutation in LLPS reporter

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1280 TDP43 RRM-GFP G348F

Plasmid

#107830

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G348F mutation in LLPS reporter

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS968 TDP43 RRM-GFP G309S

Plasmid

#107824

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G309S mutation in LLPS reporter

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS966 TDP43 RRM-GFP G309F

Plasmid

#107822

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G309F mutation in LLPS reporter

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1256 TDP43 RRM-GFP W385G

Plasmid

#107807

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

W385G mutation in LLPS reporter

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CTL2

Plasmid

#92212

Purpose

Expresses C-Terminal segment of C. elegans Piezo1 in E. coli

Depositor

Douglas Rees

Article

Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

Insert

CTL2 of PIEZO1

Tags

6x Histidine Tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
KHBD00396

Plasmid

#34507

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8961 (tef Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00410

Plasmid

#34518

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6701 (CG6701 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00187

Plasmid

#34339

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG4694 (her Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only

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