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Plasmid#99553PurposeExpresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].DepositorInsertSwi1 N-rich region (AA1-327) (SWI1 Budding Yeast)
UseTagsYFPExpressionYeastMutationPromoterTEF1Available sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHBS1398 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G368W]
Plasmid#118811PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationG368W in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pISH-Meis2
Plasmid#74349PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Meis2. Not for gene expression.DepositorInsertMeis homeobox 2 (Meis2 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation
Plasmid#58830PurposeExpresses GFP-Tagged and mutated Osh2 PH domain in yeastDepositorInsert2 x Osh2 PH domain (OSH2 Budding Yeast)
UseTagsGFP and MycExpressionYeastMutationchanged Lysine 307 to Glutamate and Arginine 309 …PromoterPHO5Available sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHBS941 TDP43 RRM-GFP VLIMFYW-S
Plasmid#121992PurposeTo test the effect of sequence on TDP43 RRM-GFP droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll hydrophobic residues in IDR1&IDR2 mutated…PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1383 TDP43 RRM-GFP FYW-S
Plasmid#118791PurposeTo test the effect of sequence on TDP43 RRM-GFP droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll FYW to S in CTD of TDP43 RRM-GFP reporterPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1386 TDP43 RRM-GFP VLIM-F
Plasmid#118794PurposeTo test the effect of sequence on TDP43 RRM-GFP droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll VLIM to F in CTD of TDP43 RRM-GFP reporterPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1387 TDP43 RRM-GFP KRED-S
Plasmid#118795PurposeTo test the effect of sequence on TDP43 RRM-GFP droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll KRED to S in CTD of TDP43 RRM-GFP reporterPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
p416TEF-NQYFP
Plasmid#99552PurposeExpresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].DepositorInsertSwi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)
UseTagsYFPExpressionYeastMutationPromoterTEF1Available sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pTriEX Tom20 mVenus BoPL
Plasmid#114467PurposeMammalian expression of mitochondrially targeted, mVenus labelled BoPLDepositorInsertBoPL
UseTagsHIS, TEV and FLAG and TOM20 and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx TOM20 mVenus BoLL
Plasmid#114465PurposeMammalian expression of mitochondrially targeted, mVenus labelled BoLLDepositorInsertBoLL
UseTagsHIS, TEV and FLAG and TOM20 and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK ENPP5 3'UTR
Plasmid#19854DepositorInsertENPP5 3'UTR (ENPP5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTriEX Tom20 mVenus BoLD
Plasmid#114468PurposeMammalian expression of mitochondrially targeted, mVenus labelled BoLDDepositorInsertBoLD
UseTagsHIS, TEV and FLAG and TOM20 and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Laccase2 Sense
Plasmid#69892PurposeExpresses the Drosophila laccase2 exon 2 circular RNADepositorInsertLaccase2 (stw Fly)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHBS931 TDP43 RRM-GFP F-S
Plasmid#107799PurposeTo test the effect of sequence on TDP43 droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll phenylalanine to serinePromoterAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLenCMV-PMMars1CyGFP
Plasmid#78315PurposeLentiviral transfer vector encoding Mars1Cy-GFP membrane-spanning proteinDepositorInsertMars1Cy-TMD-GFP
UseLentiviralTagsHA epitopeExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pBS chick LH-2B [TJ#34]
Plasmid#16271DepositorInsertLH2B (LHX9 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJJB1559
Plasmid#218590PurposeExpress peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimerDepositorInsertGES
UseTagsePTS1ExpressionYeastMutationErg20(F96W, N127W), Erg20-Erg20 synthetic homodim…PromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-Lg-B
Plasmid#216960PurposeExpression of Large BiT-Linker-GvpBDepositorInsertLarge BiT-Linker-GvpB
UseTagsFLAG-Split Nanoluciferase LargeBiTExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-B-Lg
Plasmid#216959PurposeExpression of GvpB-Linker-Large BiTDepositorInsertGvpB-Linker-Large BiT
UseTagsSplit Nanoluciferase LargeBiT-FLAGExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'
Plasmid#216877PurposeDerived from the plasmid for Mega GVs, pST39-pNL29 (Addgene #91696), but the overlap between GvpS and GvpK has been resolved.DepositorInsertDerived from Mega, and GvpS and GvpK overlap has been resolved
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1_HD_160-223AA
Plasmid#221477PurposeGateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationDeletion of 1-159AA N-terminal and 224-371AA C-te…PromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_HD_160-223AA
Plasmid#221489PurposeNKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationDeletion of 1-159AA N-terminal and 224-371AA C-te…PromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBS1506 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-L]
Plasmid#133330PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll FYW to L in CTD of full-length TDP43PromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGJM010
Plasmid#128546PurposeExpresses Chimera SFA8+BNDepositorInsertChimera SFA8+Ber e 2
UseTagsExpressionYeastMutationPromoterAOX1Available sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1397 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 W385G]
Plasmid#118812PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationW385G in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1400 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G295F]
Plasmid#118814PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationG295F in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1393 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaRRM]
Plasmid#118805PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationDeletion of RRM in full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1394 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaCTD]
Plasmid#118806PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationDeletion of CTD in full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1392 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaNTD]
Plasmid#118807PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationDeletion of NTD in full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1385 TDP43 RRM-GFP VLIM-S
Plasmid#118792PurposeTo test the effect of sequence on TDP43 RRM-GFP droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll VLIM to S in CTD of TDP43 RRM-GFP reporterPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1384 TDP43 RRM-GFP FYW-L
Plasmid#118793PurposeTo test the effect of sequence on TDP43 RRM-GFP droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll FYW to L in CTD of TDP43 RRM-GFP reporterPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL829
Plasmid#119697Purposeprecursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347CDepositorInsertslow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C
UseTagsnoneExpressionBacterialMutationmutations Y283D, G289C and V347CPromoterT5Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEX Tom20 mVenus BoPD
Plasmid#114466PurposeMammalian expression of mitochondrially targeted, mVenus labelled BoPDDepositorInsertBoPD
UseTagsHIS, TEV and FLAG and TOM20 and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS934 TDP43 RRM-GFP M-V
Plasmid#107801PurposeTo test the effect of sequence on TDP43 droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll methionine to valine in LLPS reporterPromoterAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS935 TDP43 RRM-GFP M-S
Plasmid#107800PurposeTo test the effect of sequence on TDP43 droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll methionine to serine in LLPS reporterPromoterAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1052 TDP43 RRM-GFP R-K
Plasmid#107856PurposeTo test the effect of sequence on TDP43 droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll arginine to lysine in LLPS reporterPromoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1292 TDP43 RRM-GFP G368W+W385G
Plasmid#107838PurposeTo test the effect of sequence on TDP43 droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationG368W and W385G mutations in LLPS reporterPromoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1039 TDP43 RRM-GFP 2xΦ
Plasmid#107806PurposeTo test the effect of sequence on TDP43 droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll hydrophobics combined to two clusters in LLPS…PromoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1038 TDP43 RRM-GFP 4xΦ
Plasmid#107805PurposeTo test the effect of sequence on TDP43 droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll hydrophobics combined to four clusters in LLP…PromoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1037 TDP43 RRM-GFP 6xΦ
Plasmid#107804PurposeTo test the effect of sequence on TDP43 droplet propertiesDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll hydrophobics combined to six clusters in LLPS…PromoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
CTL2 (M2225R)
Plasmid#96952PurposeExpresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coliDepositorInsertCTL2 (M222R) of PIEZO1
UseTags6x Histidine TagExpressionBacterialMutationPromoterT7 PromoterAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
p415TEF-NQYFP
Plasmid#99554PurposeExpresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].DepositorInsertSwi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)
UseTagsYFPExpressionYeastMutationPromoterTEF1Available sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 Sense
Plasmid#69883PurposeExpresses the Drosophila laccase2 exon 2 circular RNADepositorInsertLaccase2 (stw Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBEG R1-ChlorR-R3 #BGV148
Plasmid#48942PurposeContains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR3 sites for the creation of Destination vectorsDepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -