We narrowed to 1,588 results for: PFL

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LLP764_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_1IB_lacZ

Plasmid

#192408

Purpose

To act as a 1-input state of the A NIMPLY B gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP791_PB_dCas9-SSSavi_BirA

Plasmid

#211786

Purpose

PiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

dCas9, Spy, Snoop, aGCN4, AviTags

Tags

3xHA

Expression

Mammalian

Mutation

dCas9 D10A and H840A

Promoter

pCAGG abd pEF1a

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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LLP726_L2_pV01_0I_TMV-tGFP_TCTP-fLUC

Plasmid

#192406

Purpose

To be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::Turbo GFP

Use

Synthetic Biology

Tags

N7

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ

Plasmid

#192380

Purpose

To test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP1014

Plasmid

#239936

Purpose

Hsp18.2 (heat inducible) promoter driving the expression of sgRNA-B

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

Hsp18.2::sgRNA-B

Use

Synthetic Biology

Expression

Plant

Mutation

N/A

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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LLP984

Plasmid

#239906

Purpose

TCTP-SynPro-04 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-04 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP985

Plasmid

#239907

Purpose

TCTP-SynPro-05 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-05 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1020

Plasmid

#239942

Purpose

AtACT2 promoter driving the expression of dCas9-ZAT10(2x)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

AtACT2::dCas9-ZAT10(2x)

Use

CRISPR and Synthetic Biology

Expression

Plant

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1022

Plasmid

#239944

Purpose

Firefly luciferase plus as a Level-0 part (Golden Gate)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

Fluc

Use

Synthetic Biology

Tags

PEST on C-terminus of Fluc plus

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1011

Plasmid

#239933

Purpose

dCas9 without Stop codon as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

dCas9

Use

CRISPR and Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1013

Plasmid

#239935

Purpose

Csy4 recognition sequences (Cog) as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

Csy4 recognition sequence

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1005

Plasmid

#239927

Purpose

sgRNA compatible with TCTP-SynPro-02 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1006

Plasmid

#239928

Purpose

sgRNA compatible with TCTP-SynPro-02 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP982

Plasmid

#239904

Purpose

TCTP-SynPro-02 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-02 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP989

Plasmid

#239911

Purpose

TCTP-SynPro-09 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-09 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP990

Plasmid

#239912

Purpose

TCTP-SynPro-10 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-10 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP981

Plasmid

#239903

Purpose

TCTP-SynPro-01 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-01 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP983

Plasmid

#239905

Purpose

TCTP-SynPro-03 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-03 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP986

Plasmid

#239908

Purpose

TCTP-SynPro-06 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-06 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP987

Plasmid

#239909

Purpose

TCTP-SynPro-07 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-07 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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