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pRB1017

Plasmid

#59936

Purpose

empty vector for gRNA cloning

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Type

Empty backbone

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pOPINJ

Plasmid

#26045

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

HisGST3C-lacZ

Tags

MAHHHHHH_GST_LEVLFQGT

Expression

Bacterial, Insect, and Mamm…

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pGGA008

Plasmid

#48817

Purpose

Provides the alcA promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

alcA promoter

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGC011

Plasmid

#48825

Purpose

Provides alcR as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

alcR

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Tg(mpeg1:EGFP-Mmu.Rpl10a)

Plasmid

#128840

Purpose

RNA isolation from machrophages via TRAP

Depositor

Anna Huttenlocher

Article

Lam et al PLoS One. 2013 Dec 19;8(12):e84436. doi:

Insert

EGFP-L10a

Use

Zebrafish expression

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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p6-8

Plasmid

#118089

Purpose

rapa-T7- mRNA(GFP)

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

Rapamycin-inducible split T7 RNAP with T7 driven mRNA for GFP

Expression

Mammalian

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pSR1051

Plasmid

#208739

Purpose

Characterization plasmid for PZwf (sfgfp expressed from PZwf on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PZwf

Use

Synthetic Biology

Expression

Bacterial

Promoter

PZwf

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSR1057

Plasmid

#208742

Purpose

Characterization plasmid for PPgk (sfgfp expressed from PPgk on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PPgk

Use

Synthetic Biology

Expression

Bacterial

Promoter

PPgk

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDR-GW AT1.03

Plasmid

#28003

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03

Tags

mVenus and mseCFP

Expression

Yeast

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pMN cFKT

Plasmid

#18785

Depositor

Han Htun

Article

Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

Insert

TRAP (mtrB Bacillus subtilis)

Tags

6xHis and HA

Expression

Mammalian

Available Since

Aug. 12, 2008

Availability

Academic Institutions and Nonprofits only

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p5-79

Plasmid

#118087

Purpose

N-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmid

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

N-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmid

Use

Synthetic Biology

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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PLlacO-1-mfLon

Plasmid

#45152

Depositor

Michel Maharbiz

Article

Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Insert

Lon

Use

Synthetic Biology

Tags

ssRA degradation (LVA)

Expression

Bacterial

Promoter

PLlacO-1

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a- mEGFP-kanMX6

Plasmid

#105146

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mEGFP-kanMX6

Tags

mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGGA000

Plasmid

#48856

Purpose

Empty entry vector for creating GreenGate promoter modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGC025

Plasmid

#48830

Purpose

Provides 3xGFP as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

3xGFP

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSANG10-3F

Plasmid

#39264

Depositor

John McCafferty

Article

Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

Type

Empty backbone

Use

Bacterial expression, bacterial expression of re…

Tags

6xHis, pelB leader, and tri-FLAG

Expression

Bacterial

Promoter

T7

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pVH21-Tier1-PhEF1a

Plasmid

#169561

Purpose

Tier-1 PhEF1a-driven expression vector (PhEF1a-MCS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Expression

Mammalian

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGGC000

Plasmid

#48858

Purpose

Empty entry vector for creating GreenGate coding sequence modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-mYFP-hphMX6

Plasmid

#105155

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mYFP-hphMX6

Tags

mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pDM291-tetR-tup11Δ70

Plasmid

#41027

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetR-tup11Δ70

Expression

Yeast

Mutation

tup11 starts at aa70

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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