Addgene

We narrowed to 2,370 results for: buc

Showing: 701 - 720 of 2370 results

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pDONR221-hSOX10-C1240T

Plasmid

#24735

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

C replaced with T at position 1240 resulting in H…

Promoter

Available since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pDONR-hSOX10-G128A

Plasmid

#24737

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

G replaced with A at position 128

Promoter

Available since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-hSOX10-G1238A

Plasmid

#24738

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

G replaced with A at position 1238 resulting in G…

Promoter

Available since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera1

Plasmid

#79379

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Chimera 1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4

Plasmid

#79382

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Chimera 4

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pGEXtev-HMG54kd

Plasmid

#26030

Depositor

Johann Deisenhofer

Article

Istvan et al EMBO J. 2000 Mar 1. 19(5):819-30.

Insert

3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Catalytic domain (amino acids 426-488) Methion…

Promoter

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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pLEX_305-C-dTAG

Plasmid

#91798

Purpose

Lentiviral/gateway cloning vector for C-terminally tagging proteins of interest with the dTAG system

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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OA-986F (AAEL003877-dCas9-VPR)

Plasmid

#183993

Purpose

Expresses dCas9-VPR under the Ae. aegypti polyubiquitin promoter

Depositor

Omar Akbari

Article

Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

Insert

AAEL003877-dCAS9-VPR

Use

CRISPR

Tags

dCas9-VPR

Expression

Insect

Mutation

Promoter

AAEL003887

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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hAAVS1-gs4_EF1a-Cas9-2A-GFP

Plasmid

#118414

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA AAVS1 (AAVS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV:cTNT::Luciferase

Plasmid

#69915

Purpose

Used to package AAV9:cTNT::luciferase. Specifically transduce cardiomyocytes.

Depositor

William Pu

Article

Lin et al Circ Res. 2014 Jul 18;115(3):354-63. doi

Insert

Firefly luciferase

Use

AAV

Tags

Expression

Mutation

Promoter

Chicken cardiac troponin T

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1a-mCherryLuciferase-W

Plasmid

#200101

Purpose

Lentiviral vector expressing mCherry fused with Luciferase at the C-terminus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

Luciferase

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_PEmax-2A-MLH1dn-2A-BSD

Plasmid

#234072

Purpose

For lentiviral expression of PEmax with MLH1dn and blasticidin resistance

Depositor

Greg Findlay

Article

Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

Insert

PEmax-2A-MLH1dn

Use

CRISPR and Lentiviral

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

EF-1α core promoter

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/DEST/hTfR-HA

Plasmid

#69610

Purpose

human transferrin receptor with an HA tag fused at the C-terminus

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

human transferrin receptor (TFRC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pMx/ATP9B-HA

Plasmid

#209231

Purpose

Mammalian expression of ATP9B

Depositor

Hye-Won Shin

Article

Yagi et al Life Sci Alliance. 2025 Apr 15;8(7):e202

Insert

ATP9B (ATP9B Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMD19_STARR-seq_plants

Plasmid

#117379

Purpose

STARR-seq vector with 35s core promoter for transient expression in plant protoplasts

Depositor

Xiaoyu Zhang

Article

Ricci et al Nat Plants. 2019 Nov 18. pii: 10.1038/s4

Type

Empty backbone

Use

Tags

GFP derived from pMDC107

Expression

Plant

Mutation

Promoter

35s core promoter

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

Plasmid

#167901

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Tags

Expression

Mutation

D10A, D839A, H840A, N863A

Promoter

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)

Plasmid

#156463

Purpose

Express Heme Oxygenase (Nostocaceae) and ReBphP-PCM

Depositor

Andre Stiel

Article

Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

Inserts

Heme Oxygenase
ReBphP-PCM

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pB[nos-Cas9 PUb-ZsGreen]

Plasmid

#169011

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Tags

Expression

Insect

Mutation

Promoter

Drosophila melanogaster nanos

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-SpCas9-2A-GFP-noITR

Plasmid

#118415

Purpose

Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_empty

Plasmid

#160093

Purpose

Control lentiviral vector for mammalian gene expression

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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