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pET259-pUC57 24xMS2V6

Plasmid

#104391

Purpose

MS2V6 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP670

Plasmid

#45466

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-Zdk1

Plasmid

#81057

Purpose

mammalian expression of Zdk1

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, and mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pGH224_sgRNA_2xMS2_Puro

Plasmid

#85413

Purpose

hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

puromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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mOrange2-C1

Plasmid

#54650

Purpose

Localization: C1 Cloning Vector, Excitation: 549, Emission: 565

Depositor

Michael Davidson, Roger Tsien

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Tags

mOrange2

Expression

Mammalian

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pTriEx-NLyn-Venus-Zdk1

Plasmid

#81042

Purpose

mammalian expression of plasma-membrane anchored Zdk1

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, NLyn, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416I

Plasmid

#81045

Purpose

mammalian expression of LOV V416I

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV V416I

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416I

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 I427V

Plasmid

#81044

Purpose

mammalian expression of LOV2 I427V

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV I427V

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

I427V

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416T

Plasmid

#81040

Purpose

mammalian expression of LOV V416T

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2 V416T

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416T

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pBAMD1-2

Plasmid

#61564

Purpose

Standardized mini-Tn5 delivery plasmid, kanamycin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available Since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pMK344 (Hygro-P2A-mAID)

Plasmid

#121178

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-G-Creon shRNA[Control]

Plasmid

#181824

Purpose

This vector simultaneously expresses GFP protein and shRNA in a Cre-dependent manner.

Depositor

Eun Mi Hwang

Article

Kim et al J Neurosci Methods. 2021 Dec 21:109452.

Type

Empty backbone

Use

AAV and RNAi

Expression

Mammalian

Promoter

EF1a/U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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NW30

Plasmid

#133444

Purpose

Covalently circularized nanodisc NW30 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW30

Expression

Bacterial

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP

Plasmid

#84254

Purpose

Expresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GoldenGate

Plasmid

#47388

Purpose

Expression plasmid for TALE proteins compatible with GoldenGate cloning system

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

Type

Empty backbone

Use

TALEN

Tags

Flag, HA, and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pclbw-mitoTagRFP

Plasmid

#58425

Purpose

Mammalian expression of tagRFP targeted to the mitochondrial matrix

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mito-tagRFP

Use

Retroviral

Expression

Mammalian

Promoter

cmv-bactin

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pclbw-mitoCFP

Plasmid

#58426

Purpose

Mammalian expression of CFP targeted to the mitochondrial matrix

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mito-CFP

Use

Retroviral

Expression

Mammalian

Promoter

cmv-bactin

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pHR SFFVp Phy-mCherry-CAAX

Plasmid

#50839

Purpose

Optogenetic PI3K activation system; membrane component

Depositor

Wendell Lim

Article

Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

Insert

PhyB 1-908

Use

Lentiviral and Synthetic Biology

Tags

mCherry

Promoter

SFFV

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pBAMD1-6

Plasmid

#61566

Purpose

Standardized mini-Tn5 delivery plasmid, gentamicin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pDZ415 (24MS2SL loxP-Kan-loxP)

Plasmid

#45162

Depositor

Robert Singer, Daniel Zenklusen

Article

Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

Insert

24xMS2 Stem Loop Cassette

Available Since

Aug. 8, 2013

Availability

Academic Institutions and Nonprofits only

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