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pKI-cjSF1-hCD271-PGK-Neo

Plasmid

#186171

Purpose

Knock-in gene targeting in marmoset cells at SF1 locus

Depositor

Sho Yoshimatsu

Publication

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

hCD271

Use

Marmoset targeting

Mutation

human codon-optimized

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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deltaVP1 + VP2C-MIOX

Plasmid

#166680

Purpose

Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Publication

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Inserts

VP2C-MIOX
Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1 and GAL10

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 mitoAURKA Lys162Met

Plasmid

#157752

Purpose

Expression of kinase-dead AURKA K62M in mitochondria

Depositor

Marc Tramier

Publication

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Expression

Mammalian

Mutation

AURKA K162M is a kinase-dead version of AURKA

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP N1 AURKA 1-110

Plasmid

#157755

Purpose

Expression of AURKA Nter (aa1-110):GFP in mammalian cells

Depositor

Marc Tramier

Publication

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

GFP

Expression

Mammalian

Mutation

The first 110 amino acids of AURKA were cloned

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV ShadowG-AURKA K162M-mTurq2

Plasmid

#157769

Purpose

Expression of AuroraA kinase-dead biosensor under CMV promoter

Depositor

Marc Tramier

Publication

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

mTurquoise2 and shadowG

Expression

Mammalian

Mutation

AURKA K162M is a kinase-dead version of AURKA

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV ShadowY-AURKA K162M-mTurq2

Plasmid

#157771

Purpose

Expression of AuroraA kinase-dead biosensor under CMV promoter

Depositor

Marc Tramier

Publication

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

mTurquoise2 and shadowY

Expression

Mammalian

Mutation

AURKA K162M is a kinase-dead version of AURKA

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD-H253A-R256A-R260A-R296A

Plasmid

#127789

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Publication

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in His2…

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD-R385A-K386A-K389A-K390A

Plasmid

#127792

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Publication

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in Arg3…

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Khc73(1-387)-GFP

Plasmid

#31747

Depositor

Ron Vale

Publication

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Khc73 (Khc-73 Fly)

Tags

GFP

Expression

Mammalian

Mutation

Contains only aa 1-387

Promoter

Metallothionein

Available since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only
Khc73(1-432)-GFP

Plasmid

#31748

Depositor

Ron Vale

Publication

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Khc73 (Khc-73 Fly)

Tags

GFP

Expression

Mammalian

Mutation

Contains only aa 1-432

Promoter

Metallothionein

Available since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only
p3xFLAG-CMV-9-sGPR124-VEGFR2 TMD-FKBP

Plasmid

#246697

Purpose

Expression vector for human GPR124 ECD fused to human VEGFR2 TMD and human FKBP1A F36V, N-terminal 3xFLAG tag

Depositor

Calvin Kuo, Mario Vallon

Publication

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and hVEGFR2 TMD-hFKBP1A F36V

Expression

Mammalian

Mutation

ECD

Promoter

CMV

Available since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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TAPBPR S104F K211L R270Q

Plasmid

#245834

Purpose

expresses TAPBPR-S104F-K211L-R270Q in S2 cells

Depositor

Nikolaos Sgourakis

Publication

Sun et al Sci Adv. 2023 Feb 24;9(8):eade7151. doi:

Insert

TAPBPR (S104F K211L R270Q) (TAPBPL Human)

Tags

AviTag biotinylation tag and His6 affinity tag

Expression

Insect

Mutation

S104F K211L R270Q (HiFi mutants) & C94S (remo…

Promoter

MT_puro

Available since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-NR5A1-deltaLBD-FLAG

Plasmid

#242228

Purpose

Expression of human SF-1 ∆LBD (deletion of a.a. 221-459) using piggyBac transposition

Depositor

Courtney Hodges

Publication

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

NR5A1 (NR5A1 Human)

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Mutation

Deleted ligand binding domain (LBD) from SF-1 (de…

Promoter

CAG

Available since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA)

Plasmid

#240229

Purpose

Parental donor plasmid for T2A-Gal4 knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-hhN-HA

Plasmid

#240236

Purpose

Expression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

hedgehog N-terminal fragment (hh Fly)

Tags

HA-spGFP11

Expression

Insect

Mutation

aa 1-257

Promoter

UAS

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-EGFP-PA-BAX(ER, G108V)

Plasmid

#233344

Purpose

Stable expression of EGFP-LOV2(N538E)-BAX(G108V)-Cyb5a in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.

Depositor

Noboru Mizushima

Publication

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

LOV2 domain of phototropin1
BAX (BAX Human)
Cyb5a (Cyb5a Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

BAX(3-171), N-terminus and C-terminus are deleted…

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-IFIT5_K197R-VA

Plasmid

#191223

Purpose

Lentiviral expression of human IFIT5_K197R in mammalian cells

Depositor

Mohan Babu

Publication

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

changed Lysine 197 to Arginine (K197R)

Promoter

CMV

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-IFIT5_K160R-VA

Plasmid

#191222

Purpose

Lentiviral expression of human IFIT5_K160R in mammalian cells

Depositor

Mohan Babu

Publication

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

changed Lysine 160 to Arginine (K160R)

Promoter

CMV

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 ori

Plasmid

#234737

Purpose

All-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.

Depositor

Kirill Martemyanov

Publication

Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

Insert

Nrl (Nrl Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pEF2-FL-hTyro_gR1

Plasmid

#225995

Purpose

Expresses a chimeric contruct having N-terminal human Tyro3 and C-terminal human Interferon Gamma Receptor

Depositor

Raymond Birge, Sergei Kotenko

Publication

Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6

Insert

human Tyro3 N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (TYRO3, IFNGR1 Human)

Expression

Mammalian

Promoter

EF1-a

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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