We narrowed to 14,429 results for: nts

1. CoV2-N-G204R

   Plasmid

   #177953

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (G204R). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (G204R) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G204R

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMOD_B2301

   Plasmid

   #91067

   Purpose

   Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   35S

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBSFI-myr-akt-K179M

   Plasmid

   #49188

   Purpose

   cDNA of Akt1 with myristyolation signal and point mutation from K to M at position 179 in cloning vector

   Depositor

   Peter Vogt

   Article

   Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Adaptor plasmid

   Tags

   HA tag and myristolation signal

   Expression

   Mutation

   K179M

   Promoter

   T7

   Available since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-DIO-CFL-SN-P2A-GCaMP6f

   Plasmid

   #181742

   Purpose

   AAV vector for expressing CFL-SN and GCaMP6f

   Depositor

   Yasunori Hayashi

   Article

   Goto et al Science. 2021 Nov 12;374(6569):857-863.

   Insert

   CFL-SN-P2A-GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. α1Ic-HE2-pcDNA3

   Plasmid

   #45808

   Depositor

   Edward Perez-Reyes

   Article

   Murbartian et al FEBS Lett. 2002 Sep 25;528(1-3):272-8.

   Insert

   α1Ic (Cacna1i Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAFW-Ago1

   Plasmid

   #50553

   Purpose

   Expression of FLAG tagged Ago1 in S2 cell

   Depositor

   Yukihide Tomari

   Article

   Kawamata et al Nat Struct Mol Biol. 2009 Sep;16(9):953-

   Insert

   Argonaute1 (AGO1 Fly)
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   Feb. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMP71-mIL15-IL12rb1-sushi-il12rb2

   Plasmid

   #174606

   Purpose

   Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMOD_C2517

   Plasmid

   #91086

   Purpose

   Module C, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   TaU3

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOD_B2615

   Plasmid

   #91078

   Purpose

   Module B, Promoter: AtU6, Gene: BsaI ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   BsaI ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   AtU6

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. (326) pAAV SV40-ZsGreen U6-gRNA

    Plasmid

    #163020

    Purpose

    Fluorescent reporter for Sa gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Zs Green
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. plc242-GFP-WDR24

    Plasmid

    #87050

    Purpose

    mammalian expression of WDR24

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    WDR24 (WDR24 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. ZIKV_Sensor_32B_LacZ

    Plasmid

    #75007

    Purpose

    Toehold switch sensor to detect Zika Virus

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_sensor_32B_LacZ
    

    Use

    Tags

    LacZ

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Venus-7aa-ActA-pEGFP-C1

    Plasmid

    #177405

    Purpose

    To express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.

    Depositor

    David Andrews

    Article

    Osterlund et al Protocol Exchange (2022)

    Insert

    ActA tail anchor (actA Listeria monocytogenes)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe puro H-Ras G12V Y40C

    Plasmid

    #12276

    Depositor

    Bob Weinberg

    Article

    Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

    Insert

    H-Ras G12V Y40C (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Contains G12V activating mutation. The Y40C mutat…

    Promoter

    Available since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

15. mCerulean-LaminB1-10

    Plasmid

    #55380

    Purpose

    Localization: Nuclear Envelope, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.2 mir-1-1 reporter hsa-mir-122

    Plasmid

    #46673

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-122 (MIR122 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV Myc P57S IRES GFP

    Plasmid

    #18771

    Depositor

    Scott Lowe

    Article

    Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

    Insert

    myc P57S (MYC Human)
    

    Use

    Retroviral

    Tags

    IRES GFP

    Expression

    Mammalian

    Mutation

    P57S

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pOPS0378

    Plasmid

    #133229

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAT15415-BEAR-GFP-target-mCherry

    Plasmid

    #162995

    Purpose

    plasmid expressing an sgRNA targeting the BEAR-GFP plasmid along with an mCherry marker

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    sgRNA targeting the BEAR-GFP plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJ3 SHP2 E76A

    Plasmid

    #8321

    Depositor

    Ben Neel

    Article

    Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E76A (activated mutant)

    Promoter

    Available since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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