We narrowed to 19,051 results for: Tec;

1. SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)

   Plasmid

   #194222

   Purpose

   Mammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptors

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   SynNotch-GAL4 DBD-VP64 AD
   

   Use

   Synthetic Biology

   Promoter

   None

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cas12f-GE ver3.0

   Plasmid

   #176508

   Purpose

   Expresses Cas12f-GE in mammalian cells

   Depositor

   Yong-Sam Kim

   Article

   Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

   Inserts

   Cas12f-GE ver3.0
   Cas12f-GE ver3.0
   

   Expression

   Mammalian

   Promoter

   U6 and chicken β-actin

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EFS-DIO-GRAB_OTmut

   Plasmid

   #185389

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   GPCR activation based oxytocin control sensor GRAB_OTmut
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOCC304

   Plasmid

   #118897

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with TEVprotease and TwinStrep-MB…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL0812 (pCRISPR(BbsI)_Vch)

   Plasmid

   #200898

   Purpose

   Expresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCRISPR (entry)
   

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pK1E-RNAP

   Plasmid

   #48144

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   rna polymerase K1E
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)

   Plasmid

   #181790

   Purpose

   Expresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
   

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_eA3A-nCas9

   Plasmid

   #163535

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSaPIPLC His

   Plasmid

   #173810

   Purpose

   Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells

   Depositor

   Anne Gershenson, Mary F Roberts

   Article

   Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87

   Insert

   Staphylococcus aureus plc
   

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry

    Plasmid

    #136603

    Purpose

    Encodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

    Depositor

    John Ngo

    Article

    Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

    Insert

    FKBP-C-mSOG-J-alpha
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUSE-RapR-Src(KD)-myc

    Plasmid

    #25934

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    RapR-Src (Fkbp1a Mouse)
    

    Expression

    Mammalian

    Mutation

    This pUSE-RapR-Src(KD)-myc plasmid contains the k…

    Available Since

    Oct. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-ABE-NT-sgRNA

    Plasmid

    #112734

    Purpose

    AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA

    Depositor

    Jin-Soo Kim

    Article

    Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

    Insert

    ABE-NT-sgRNA
    

    Use

    AAV

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-eGFP-dSaCas9-blast

    Plasmid

    #191653

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    eGFP-T2A-dSaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfB3052(gRNA X-4, XI-3, XII-5)

    Plasmid

    #73294

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGS1021

    Plasmid

    #59575

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
    

    Use

    E. coli cloning vector

    Tags

    Twin-Strep tag

    Promoter

    None

    Available Since

    Aug. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLCHKO

    Plasmid

    #155048

    Purpose

    Lentiviral backbone for cloning and expressing U6 driven hybrid guide (hg)RNAs with BfuAI cloning sites and puromycin selection.

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry

    Plasmid

    #67668

    Purpose

    Zebrafish expression vector for ubiquitous expression of APEX2-GBP

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-GBP
    

    Use

    Zebrafish expression with tol2

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-hA3F-BE3

    Plasmid

    #113414

    Purpose

    Expresses hA3F-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3F-BE3 (APOBEC3F S. pyogenes and Bacteriophage PBS2, Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSF-G1(6CNW)RS

    Plasmid

    #222873

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRB117.2

    Plasmid

    #181949

    Purpose

    aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    mNG-NarL; mCherry; tetR (narL, AX04_RS23485 Synthetic, narL - E. coli)
    

    Tags

    mNeonGreen

    Expression

    Bacterial

    Mutation

    See Depositor Comments Below

    Promoter

    mNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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