We narrowed to 20,626 results for: emb
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Plasmid#49942PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only
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pCS2 xenopus Frzb-1
Plasmid#24694DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFrzb-1 (frzb-1 Frog)
ExpressionMammalianAvailable sinceJuly 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
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InterTag-3xL
Plasmid#186913PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side expressing BFP and puromycin resistance geneDepositorInsertSpyTag003-sfCherry11-(GGGGS)3-PDGFR
ExpressionMammalianAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-FKBP-PIP5K1C_Kina-HDD316K
Plasmid#202742Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmTagBFP2:FKBP1A (3-108) : [GGSA]4GG : PIP5K1C(79-366) (D101R / R304D / D316K) (PIP5K1C Human)
ExpressionMammalianAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-p18-CIBN
Plasmid#188990PurposeA lentiviral plasmid encoding lysosome-tagged CIBNDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCIBN (CIB1 Mustard Weed)
Tagsp18ExpressionMammalianPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPW3755 : HDR-HsIRE1a-10xGS-HaloTag
Plasmid#185677PurposeHDR template used for endogenous CRISPR editing of ERN1 gene, inserting a C-terminal HaloTag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertERN1 homology arms (ERN1 Synthetic, Human)
UseCRISPRTagsHaloTagAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax
Plasmid#190153PurposeExpresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vectorDepositorInsertVamp2 (Vamp2 Mouse)
UseAAVTagsP2AT2A-EGFP-caaxExpressionMammalianMutationTruncation that includes only AA #1-94PromoterMBPAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-RHOA_sgRNA
Plasmid#183878PurposepX459V2.0-HypaCas9 plasmid with RHOA sgRNA for N-terminal tagging of RhoA in human cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRHOA sgRNA spacer (RHOA Human)
UseCRISPRExpressionMammalianPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-Rnd3 promoter [-1759 +248]
Plasmid#86438PurposeFirefly luciferase reporter under control of the Rnd3 promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRnd3 promoter (RND3 Human)
UseLuciferaseTagsLuciferase (under Rnd3 promoter control)ExpressionMammalianMutationpromoter sequence derived from HepG2 cellsAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
CD40-bio-His
Plasmid#51648PurposeExpresses full-length Tumor necrosis factor receptor superfamily member CD40 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertCD40 molecule (CD40 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
nes374tk/lacZ
Plasmid#47615Purposeused to create transgenic mice expressing LacZ reporter with Nestin enhancerDepositorInsertnestin 2nd intron fragment (NES Human)
UseEnhancer reporterTagsHSV tk promoter and lacZMutation374 bp from 2nd intron (comprising bp 1153 to 152…PromoterHSV tk promoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
InterTag-BFP
Plasmid#186901PurposeCell-free expression of transmembrane protein PDGFR-tagBFP-sfCherry11-SpyTagDepositorInsertSpyTag003-sfCherry11-tagBFP-PDGFR
UseSynthetic BiologyAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterCatch-GFP
Plasmid#186900PurposeCell-free expression of transmembrane protein TfR-sfGFP-SpyCatcher-sfCherry1-10DepositorInsertTfR-sfGFP-SpyCatcher003-sfCherry1-10
UseSynthetic BiologyAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
BASIC_5_Amp
Plasmid#68139Purposestorage vector for BASIC part: AmpDepositorPublicationInsertAmpicillin
ExpressionBacterialAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
BASIC_1_MB1
Plasmid#68135Purposestorage vector for BASIC part: MB1DepositorPublicationInsertMB1 origin
ExpressionBacterialAvailable sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2+/N-Halo
Plasmid#184417Purposevector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesisDepositorTypeEmpty backboneUseFor mrna synthesisTagsHalo-tagMutationCodon optimized for expression in Xenopus laevis …Available sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAX-EGFP-FBP17
Plasmid#178365PurposeExpresses FBP17 c-terminal labeled with EGFP in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFormin Binding Protein 17 (FNBP1 Human)
ExpressionMammalianPromoterChicken Beta ActinAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-INPP4A
Plasmid#171944PurposeExpresses INPP4A in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertINPP4A (INPP4A Human)
TagsEGFPExpressionMammalianMutationSee Depositor Comments BelowAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-optimizedNedd4.2d
Plasmid#165103Purposebacterial expression of a GST fusion of Human Nedd4.2dDepositorInsertNedd4.2d (NEDD4L Human)
TagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expressionAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only