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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,306 results for: CAR

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Showing: 7241 - 7260 of 7306 results

  1. pLX-EF1a_NKX2-1_HD_160-223AA

    Plasmid
    #221489
    Purpose
    NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation lentiviral overexpression
    Depositor
    Matthew Meyerson
    Publication
    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.
    Insert
    NKX2-1 (NKX2-1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Deletion of 1-159AA N-terminal and 224-371AA C-te…
    Promoter
    EF1a
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    221489-plasmid-map-sequence-id-437749

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  2. pWR63(YUM1)

    Plasmid
    #203336
    Purpose
    episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis
    Depositor
    J. Brian Robertson
    Publication
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    CaCas9
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    THD3 promoter (S. stipitis)
    tRNA-sgRNA(SapI)-HDV
    ADH1 terminator (S. stipitis)
    YUM1-targeting sequence
    Use
    CRISPR
    Expression
    Yeast
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    203336-plasmid-map-sequence-id-417292

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  6. t1-405(151-154AAAA)

    Plasmid
    #166131
    Purpose
    Expression of human talin-1 head (aa1-405) in E. coli. Four substitutions in the F1-loop (aa151-154 all mutated to Al). N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site.
    Depositor
    Vesa Hytönen
    Publication
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    T1head1-405(151-154AAAA) (TLN1 Human)
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    aa1-405 only. Contains 4 amino acid substitutions…
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166131-plasmid-map-sequence-id-330282

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  7. Donor-CD55-NeoR

    Plasmid
    #153551
    Purpose
    Used as a donor vector for the targeted knock-in of a CD55 mutation. This plasmid carries a neomycin resistance gene cassette
    Depositor
    Hiroyuki Konishi
    Publication
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    mutant CD55 (a 1415-bp fragment from intron 3, exon 4 and intron 4, with a 1-bp mutation in exon 4), divided into 5' and 3' arms
    Use
    AAV and Cre/Lox; Donor plasmid/targeting vector
    Tags
    N/A
    Mutation
    a 1-bp mutation in exon 4
    Promoter
    No promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    153551-plasmid-map-sequence-id-326384

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  8. Donor-PIGA-NeoR

    Plasmid
    #153549
    Purpose
    Used as a donor vector for the targeted knock-in of a PIGA mutation. This plasmid carries a neomycin resistance gene cassette
    Depositor
    Hiroyuki Konishi
    Publication
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6), divided into 5' and 3' arms
    Use
    AAV and Cre/Lox; Donor plasmid/targeting vector
    Tags
    N/A
    Mutation
    a 3-bp mutation in exon 6
    Promoter
    No promoter
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    153549-plasmid-map-sequence-id-319273

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  14. pKP134 [pRS406-YBR139Wp-YBR139W(S219,D415,H474A)-GFP-ADH1t]

    Plasmid
    #106469
    Purpose
    Expresses Atg42/Ybr139w(S219,D415,H474A) with a C-terminal GFP tag in yeast cells
    Depositor
    Daniel Klionsky
    Publication
    Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109
    Insert
    YBR139W(S219,D415,H474A)
    Tags
    GFP
    Expression
    Bacterial and Yeast
    Mutation
    Changed Serine 219, Aspartate 415 and Histidine 4…
    Promoter
    YBR139W
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    106469-plasmid-map-sequence-id-204903

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  16. pHM1 KGA-33A

    Plasmid
    #227836
    Purpose
    Used for microinjection of nematodes for expression of KGA-33A, GLS with 33 residues mutated to alanine at the interface for interacting with PDZD8
    Depositor
    Sheng-cai Lin
    Publication
    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414
    Insert
    KGA isoform (GLS Human)
    Tags
    GFP
    Expression
    Worm
    Mutation
    P137, L139, E140, L142, Y145, G150, Q151, E152, K…
    Available since
    July 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    227836-plasmid-map-sequence-id-479443

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  17. Lenti-SAM-Cre

    Plasmid
    #198714
    Purpose
    For expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cells
    Depositor
    Tyler Jacks
    Publication
    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1
    Inserts
    U6-MS2-gRNAscaffold
    Pgk-MPH-Cre
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Promoter
    Pgk and U6
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    198714-plasmid-map-sequence-id-402628

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Showing: 7241 - 7260 of 7306 results