We narrowed to 17,340 results for: EMB

1. pNeuroD-ires-GFP

   Plasmid

   #61403

   Purpose

   Mammalian expression vector under the neuron-specific promoter NeuroD. Co-expression of GFP with IRES sequence

   Depositor

   Franck Polleux

   Article

   Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   NeuroD

   Available Since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. Traptavidin-E6

   Plasmid

   #59549

   Purpose

   pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

   Insert

   Core Traptavidin-E6
   

   Tags

   E6 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pDG04439

   Plasmid

   #216365

   Purpose

   xhNup133-Nb2t (+2Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup133-Nb2t (+2Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SP-GFP-CD274-UTR

   Plasmid

   #121142

   Purpose

   Mammalian expression of GFP tagged CD274 with its own 3'UTR

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   CD274 with its own 3'UTR (CD274 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV5B-TGFbeta receptor I K232R

   Plasmid

   #11763

   Depositor

   Jeff Wrana

   Article

   Wieser et al EMBO J. 1995 May 15. 14(10):2199-208.

   Insert

   TGFbeta receptor I (TGFBR1 Human)
   

   Tags

   6xHIS

   Expression

   Mammalian

   Mutation

   K232R: kinase dead

   Promoter

   CMV

   Available Since

   May 12, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. Sox1/2/3-2373/+3>nls::Cas9::nls

   Plasmid

   #59989

   Purpose

   Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   nls::Cas9::nls
   

   Use

   CRISPR

   Mutation

   Reverted mutations from dCas9 to wiltdtype

   Promoter

   Ciinte.Sox1/2/3 (SoxB1) -2373/+3

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK453 (BSD-P2A-dTAG-mCherry)

   Plasmid

   #214383

   Purpose

   dTAG-mCherry for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BSD-P2A-dTAG-mCherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC118-FLIP-FlpE-Puro

   Plasmid

   #84539

   Purpose

   a mock vector for generating FLIP-FlpE targeting vectors

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

   Insert

   FLIP-FlpE - Puro
   

   Use

   Cre/Lox and Mouse Targeting

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCC25

   Plasmid

   #177319

   Purpose

   Expression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coli

   Depositor

   Rafal Tomecki

   Article

   Tomecki et al RNA Biol. 2021 Nov 12;18(sup2):623-639.

   Insert

   TtCutA 240-603
   

   Tags

   6xHis-SUMOTag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. SB_concave

    Plasmid

    #110102

    Purpose

    pBXPHM3 with scaffold Sybody of the concave library

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Insert

    scaffold Sybody of the concave library
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOGG012

    Plasmid

    #113986

    Purpose

    Vector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Partition genes (parABCDE)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMA-SpCas9-g1

    Plasmid

    #80784

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMA-SpCas9-g4

    Plasmid

    #80787

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSC226

    Plasmid

    #216375

    Purpose

    xhNup358-Nb2t (+3Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup358-Nb2t (+3Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBN1

    Plasmid

    #86710

    Purpose

    C. elegans injection marker

    Depositor

    Peter Askjaer

    Article

    Rodenas et al Mol Biol Cell. 2012 Mar;23(5):930-44. do

    Insert

    Plmn-1::mCherry::his-58::pie-1 3'UTR
    

    Expression

    Worm

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. PEP116E

    Plasmid

    #97402

    Purpose

    binary construct of plasma membrane-targeted mCherry with sfGFP11 tag for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    mCherry-sfGFP11
    

    Tags

    Plasma membrane target

    Expression

    Plant

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Skp

    Plasmid

    #213139

    Purpose

    Expresses chaperone protein SKP

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    SKP (21-161) (skp E.coli)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP hSmad4-HomDonor

    Plasmid

    #206044

    Purpose

    Donor plasmid for CRISPR knock-in

    Depositor

    Ali Hemmati Brivanlou

    Article

    Nemashkalo et al Development. 2017 Sep 1;144(17):3042-305

    Insert

    Smad4 (SMAD4 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXs-Oct3/4-IP

    Plasmid

    #15918

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. T7-RelA(K310R)

    Plasmid

    #23250

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    NFkB RelA (RELA Human)
    

    Tags

    T7 gp10

    Expression

    Mammalian

    Mutation

    K310R

    Available Since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only