We narrowed to 9,277 results for: Pol;

1. pLVX-EF1alpha-SARS-CoV-2-nsp15-2xStrep-IRES-Puro

   Plasmid

   #141381

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-nsp15 (ORF1ab SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available Since

   April 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pLVX-EF1alpha-SARS-CoV-2-orf10-2xStrep-IRES-Puro

   Plasmid

   #141394

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-orf10 (ORF10 SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available Since

   April 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf7b-IRES-Puro

   Plasmid

   #141389

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-orf7b (ORF7b SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available Since

   April 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLVX-EF1alpha-SARS-CoV-2-nsp11-2xStrep-IRES-Puro

   Plasmid

   #141377

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-nsp11 (ORF1ab SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available Since

   April 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. MutaT7

   Bacterial Strain

   #156460

   Purpose

   An E. coli strain that expresses MutaT7 for mutagenizing genes downstream from a T7 promoter.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Bacterial Resistance

   Streptomycin

   Species

   Rattus norvegicus

   Available Since

   Oct. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

6. FW102 OL2-62

   Bacterial Strain

   #53735

   Purpose

   Bacterial-two hybrid reporter strain

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Bacterial Resistance

   Kanamycin and streptomycin

   Species

   Escherichia coli

   Available Since

   June 18, 2014

   Availability

   Academic Institutions and Nonprofits only

7. drApo1–T7

   Bacterial Strain

   #156461

   Purpose

   An E. coli strain that expresses an inactive variant of MutaT7. Used as a control strain for mutagenesis experiments with MutaT7.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Bacterial Resistance

   Streptomycin

   Species

   Rattus norvegicus

   Available Since

   Oct. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

8. rEcoli XpS

   Bacterial Strain

   #192872

   Purpose

   MG1655-C321 -- All 321 UAG codons changed to UAA

   Depositor

   Jesse Rinehart

   Article

   Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

   Bacterial Resistance

   Bleocin (zeocin)

   Species

   Escherichia coli

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

9. CC1215

   Bacterial Strain

   #79062

   Purpose

   BL21(DE3)-based E. coli purine auxotrophic host strain used for the preparation of PurE proteins in a purE background.

   Depositor

   T. J. Kappock

   Bacterial Resistance

   Kanamycin

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pLEW13

    Plasmid

    #24007

    Depositor

    George Cross

    Insert

    T7 RNA Polymerase
    

    Use

    Trypanosome expression

    Available Since

    May 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcGFP-IVT

    Plasmid

    #205145

    Purpose

    IVT template of AcGFP with MCS in frame and polyA

    Depositor

    Igor Ulitsky

    Insert

    AcGFP1
    

    Use

    In-vitro transcription

    Promoter

    T7

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2FLAG

    Plasmid

    #16331

    Depositor

    Peter Klein

    Insert

    Flag tag
    

    Use

    Vertebrate expression

    Tags

    Flag

    Mutation

    Flag tag inserted into pCS2+ polylinker

    Available Since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcGFP-stop-IVT

    Plasmid

    #205146

    Purpose

    IVT template of AcGFP stop codon between the ORF and MCS and polyA

    Depositor

    Igor Ulitsky

    Insert

    AcGFP1
    

    Use

    In-vitro transcription

    Promoter

    T7

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOpen-T4 PNK

    Plasmid

    #165520

    Purpose

    The most commonly used polynucleotide kinase.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    T4 PNK
    

    Use

    Synthetic Biology

    Available Since

    Aug. 3, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. SV40-MCS-Z1

    Plasmid

    #127639

    Purpose

    Empty backbone plasmid with SV40 promoter and SV40 polyA with Zeocin/Bleocin bacterial resistance

    Depositor

    Jordan Green

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    SV40 promoter

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAPS1 R944L mKate2

    Plasmid

    #244994

    Purpose

    Rat bipolar mutant CAPS1 R994L

    Depositor

    Thomas Martin

    Insert

    CADPS1
    

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    R944L

    Available Since

    Oct. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRLuc-IVT

    Plasmid

    #205148

    Purpose

    IVT template of Renilla luciferase stop codon between the ORF and MCS and polyA

    Depositor

    Igor Ulitsky

    Insert

    Renilla Luciferase
    

    Use

    Luciferase; In-vitro transcription

    Promoter

    T7

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOpen-TthRT

    Plasmid

    #165566

    Purpose

    DNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    Thermus thermophilus (Tth) RT
    

    Use

    Synthetic Biology

    Available Since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pAcRP67A prM/E YFV

    Plasmid

    #52883

    Purpose

    expression of Yellow Fever Virus VLPs in baculovirus

    Depositor

    Yorgo Modis

    Insert

    prM/E
    

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

20. Hsp68-CreER(TM) (DM#264)

    Plasmid

    #15028

    Depositor

    Douglas Melton

    Insert

    Cre-ER(TM)
    

    Use

    Cre/Lox; For making transgenic constructs

    Tags

    Hsp68 promoter and polyA

    Expression

    Mammalian

    Available Since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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