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HsCLYBL-FLAG

Plasmid

#111290

Purpose

mammalian expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

human CLYBL (CLYBL Human)

Tags

linker and FLAG tag: TGGGSGGSGGSMDYKDDDDK

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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HsCLYBL(D320A)-FLAG

Plasmid

#111291

Purpose

mammalian expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

human CLYBL D320A (CLYBL Human)

Tags

linker and FLAG tag: TGGGSGGSGGSMDYKDDDDK

Expression

Mammalian

Mutation

D320A

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB4 (kinase dead)

Plasmid

#29533

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

changed lysine 751 to methionine

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
EGFP-hPMCA2x/b

Plasmid

#47585

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

pMCA2x/b (ATP2B2 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-mCherry.3xFLAG.NOS1AP-WPRE

Plasmid

#127864

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Promoter

hSyn

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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FCK-ArchT-GFP

Plasmid

#31177

Purpose

Lentiviral expression of ArchT-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pSico-shRaptor

Plasmid

#81086

Purpose

conditional expression of an shRNA targeting murine Raptor

Depositor

Angelique Bordey

Article

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

Raptor shRNA (Rptor Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pEX-CMV-SP-CFP-STM2(15-746)

Plasmid

#18863

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

Stromal Interaction Molecule 2 (STIM2 Human)

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted between signal peptide and residues …

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_Sik3_T142Q

Plasmid

#119973

Purpose

Lentiviral expression plasmid of mouse Sik3 cDNA (gatekeeper mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

Sik3 (Sik3 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

change Threonine 142 to Glutamine

Promoter

EFS promoter

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEasyG1_hph

Plasmid

#184912

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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ATG9A-2STrep

Plasmid

#210359

Purpose

Expresses ATG9A fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

ATG9A (ATG9A Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-Neo-gp78/JM20

Plasmid

#13303

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78 (AMFR Human)

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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GLG1-2Strep

Plasmid

#210362

Purpose

Expresses GLG1 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

GLG1 (GLG1 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pMCS.71/2272

Plasmid

#22732

Depositor

Mark Magnuson

Article

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox71-MCS-Lox2272

Use

Cre/Lox

Mutation

This is a basal exchange vector for RMCE containi…

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TREK-2

Plasmid

#224769

Purpose

In vitro transcription for Oocyte expression of 1 TREK-2

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 10 (Kcnk10 Mouse)

Use

In vitro transcription for oocyte expression

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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NSP3C-Flag

Plasmid

#210349

Purpose

Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

C terminal of SARS-CoV-2 NSP3 (ORF1ab )

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_M337P

Plasmid

#98675

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337P

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Mutation

M337P

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_A326P

Plasmid

#98672

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with A326P

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Mutation

A326P

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-hPMCA2z/b

Plasmid

#47584

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

pMCA2z/b (ATP2B2 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only
Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]

Plasmid

#26569

Depositor

Diane Lipscombe

Article

Bell et al Neuron. 2004 Jan 8. 41(1):127-38.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Deletion of exons a10, 18a, and 24a. Contains …

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only