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PRK5 I7_pECIA14

Plasmid

#114782

Purpose

Prey vector PRK5 I7_pECIA14 should be used with bait vector PRK5 I7_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G50610

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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MEE39 N5_pECIA14

Plasmid

#114754

Purpose

Prey vector MEE39 N5_pECIA14 should be used with bait vector MEE39 N5_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G46330

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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RHS16 N12_pECIA14

Plasmid

#114760

Purpose

Prey vector RHS16 N12_pECIA14 should be used with bait vector RHS16 N12_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G29180

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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SERK4 A7_pECIA14

Plasmid

#114770

Purpose

Prey vector SERK4 A7_pECIA14 should be used with bait vector SERK4 A7_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G13790

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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SERK5 A8_pECIA14

Plasmid

#114771

Purpose

Prey vector SERK5 A8_pECIA14 should be used with bait vector SERK5 A8_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G13800

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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NIK2 A9_pECIA14

Plasmid

#114773

Purpose

Prey vector NIK2 A9_pECIA14 should be used with bait vector NIK2 A9_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G25560

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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SARK A10_pECIA14

Plasmid

#114774

Purpose

Prey vector SARK A10_pECIA14 should be used with bait vector SARK A10_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G30520

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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IOS1 A1_pECIA14

Plasmid

#114736

Purpose

Prey vector IOS1 A1_pECIA14 should be used with bait vector IOS1 A1_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G51800

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Arg-SH2

Plasmid

#46405

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Arg/Abl2 (ABL2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 151-257

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX chimerin-SH2

Plasmid

#46415

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

chimerin (CHN1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 44-147

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Dapp1-SH2

Plasmid

#46422

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Dapp1 (DAPP1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 25-151

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 Slp2-a C2AB

Plasmid

#40058

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp2-a C2AB (Sytl2 Mouse)

Tags

GST

Expression

Bacterial

Mutation

C2AB domains only

Promoter

Tac

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75234

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75235

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX Mist-SH2

Plasmid

#46454

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Mist (Clnk Mouse)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 300-435

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pKK-mVenus-TEV

Plasmid

#105774

Purpose

Expression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

mVenus-TEV

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Hook3(1-522)-GFP-Psc-StrepII

Plasmid

#111861

Purpose

C-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

Hook3(1-552)-GFP (HOOK3 Human)

Tags

GFP, Psc tag, and Strep tag

Expression

Insect

Mutation

Optimised for expression in SF9 cells

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS1920

Plasmid

#82562

Purpose

High copy number plasmid containing Ple253 (PITX3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

Ple253

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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Hy_pMT Laccase2 MCS-HIPK3 Exon

Plasmid

#69888

Purpose

Expresses the human HIPK3 exon 2 circular RNA in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

HIPK3 (HIPK3 Fly, Human)

Expression

Insect

Mutation

Expresses aa1-194 of HIPK3

Promoter

Metallothionein Promoter (pMT)

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-T,TreR-T]

Plasmid

#60775

Purpose

Contains PIq driving expression GalS-T, and PI driving expression of TreR-T.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-T
TreR-T

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. TAN DBD with GalS LB…

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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