We narrowed to 187,406 results for: ins

1. pENTR-Sensor_P2A_SLC2A4

   Plasmid

   #221429

   Purpose

   Gateway entry clone encoding a polyprotein that is cleaved to FRET glucose sensor and SLC2A4 proteins

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   Sensor_P2A_SLC2A4 (SLC2A4 Synthetic)
   

   Expression

   Mammalian

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TH1203-pOCC177_pOEM1-N-HIS-MBP-PS-TAF15_opt-TEV-SNAP

   Plasmid

   #221891

   Purpose

   Shuttle vector for baculovirus production, using FlashBac bacmid

   Depositor

   Anthony Hyman

   Article

   Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

   Insert

   TAF15 (TAF15 Human)
   

   Tags

   6xHis-MBP-PreScission and TEV-SNAP-tag

   Expression

   Insect

   Mutation

   wt

   Promoter

   PH promoter

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-XPACK-DNAJC5 (L115R)- HaloTag

   Plasmid

   #205712

   Purpose

   Mammalian expression of XPACK-DNAJC5 (L115R)-HaloTag

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Tags

   HaloTag and XPACK (GCINSKRKD)

   Expression

   Mammalian

   Mutation

   L115R

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)

   Plasmid

   #208974

   Purpose

   Recruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   B103-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)

   Plasmid

   #208971

   Purpose

   Recruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)

   Plasmid

   #208970

   Purpose

   Recruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29(+exo)-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV_PGK_3xHA-LTB4R(T308A)

   Plasmid

   #208092

   Purpose

   Mammalian expression of the human LTB4 receptor LTB4R with the T308A mutation with an N-terminal 3x HA tag.

   Depositor

   Sean R Collins

   Article

   Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

   Insert

   3xHA-LTB4R(T308A) (LTB4R Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   T308A

   Promoter

   PGK

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV_PGK_3xHA-LTB4R(S310A)

   Plasmid

   #208093

   Purpose

   Mammalian expression of the human LTB4 receptor LTB4R with the S310A mutation with an N-terminal 3x HA tag.

   Depositor

   Sean R Collins

   Article

   Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

   Insert

   3xHA-LTB4R(S310A) (LTB4R Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   S310A

   Promoter

   PGK

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV_PGK_3xHA-LTB4R(T308A,S310A)

   Plasmid

   #208094

   Purpose

   Mammalian expression of the human LTB4 receptor LTB4R with the T308A and S310A mutations with an N-terminal 3x HA tag.

   Depositor

   Sean R Collins

   Article

   Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

   Insert

   3xHA-LTB4R(T308A,S310A) (LTB4R Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   T308A, S310A

   Promoter

   PGK

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. ACVR1-TASA-R206H

    Plasmid

    #206182

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S190A, S194A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Mutation

    S190A, S194A, R206H

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. ACVR1-TSAA-R206H

    Plasmid

    #206183

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S192A, S194A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Mutation

    S192A, S194A, R206H

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. ACVR1-VSAS-R206H

    Plasmid

    #206185

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S192A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Mutation

    T189V, S192A, R206H

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. ACVR1-VASS-R206H

    Plasmid

    #206186

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S190A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Mutation

    T189V, S190A, R206H

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET32a-hEPGN

    Plasmid

    #199232

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Epithelial mitogen isoform 6 (EPGN Human)
    

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSF-G1TMSNKRS

    Plasmid

    #198323

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Ekanayake et al J Med Chem. 2023 Mar 15. doi: 10.1021/ac

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-MYO10-FERM-ITGBD

    Plasmid

    #194855

    Purpose

    Express EGFP-MYO10 with mutations (S2001_F2002insA/T2009D) that interfere with ITGB binding in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

    Insert

    MYO10 S2001_F2002insA/T2009D (MYO10 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    MYO10 has the following mutations S2001_F2002insA…

    Promoter

    CMV

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRRL.SIN.EF1A.JAG1-F1.GS.CAR-3G

    Plasmid

    #194462

    Purpose

    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.

    Depositor

    Ana Sofia Coroadinha

    Article

    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

    Insert

    Anti-JAG1 CAR
    

    Use

    Lentiviral

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRRL.SIN.EF1A.JAG1-F1.218.CAR-3G

    Plasmid

    #194460

    Purpose

    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

    Depositor

    Ana Sofia Coroadinha

    Article

    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

    Insert

    Anti-JAG1 CAR
    

    Use

    Lentiviral

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Coexpressed VLP NES

    Plasmid

    #182433

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS
    deltaVP1
    VP2C-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1, GAL10, and GAL7

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Coexpressed VLP NES (M)

    Plasmid

    #182435

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS(M)
    deltaVP1
    VP2C-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1, GAL10, and GAL7

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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