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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,284 results for: crispr

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Showing: 7321 - 7340 of 25284 results

  1. pJAT57

    Plasmid
    #204303
    Purpose
    Expresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles.
    Depositor
    David Stern
    Publication
    Stern et al bioRxiv 2023.06.17.545412
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MHC-DsRed
    Use
    CRISPR
    Promoter
    MHC
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204303-plasmid-map-sequence-id-405488

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  2. pYZ675

    Plasmid
    #132954
    Purpose
    crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a Schizosaccharomyces pombe
    Depositor
    Jef Boeke
    Publication
    Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578
    Insert
    dFnCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    nucleoplasmin NLS
    Promoter
    adh1
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    132954-plasmid-map-sequence-id-307989

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  3. pYZ694

    Plasmid
    #132956
    Purpose
    crRNA entry vector of Prrk1-Not1-HHR with dFnCas12a-Clr4 in Schizosaccharomyces pombe
    Depositor
    Jef Boeke
    Publication
    Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578
    Insert
    dFnCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS and nucleoplasmin NLS
    Promoter
    adh1
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    132956-plasmid-map-sequence-id-307975

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  6. pPE4max-Blasticidin

    Plasmid
    #194905
    Purpose
    Expressing prime editing PE4max proteins, to work in conjunction with an epegRNA encoding plasmid or Circular Vector as described in the cited article.
    Depositor
    George Church
    Publication
    Oliynyk et al Commun Biol. 2022 Dec 21;5(1):1393. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BSD
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    194905-plasmid-map-sequence-id-383725

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  10. PM-SP!TB

    Plasmid
    #48650
    Purpose
    Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bacterial SP crRNA to prototspacer B
    Use
    CRISPR
    Promoter
    J23100 promoter
    Available since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    48650-plasmid-map-sequence-id-71696

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  11. miniTurbo-dCasRx

    Plasmid
    #219821
    Purpose
    EF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCasRx
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Promoter
    EF1a and hU6
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    219821-plasmid-map-sequence-id-440859

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  12. BFP Homology Donor

    Plasmid
    #149372
    Purpose
    Promoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection
    Depositor
    Tarek Abbas
    Publication
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CDDR BFP Homology Donor
    Expression
    Mammalian
    Available since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    149372-plasmid-map-sequence-id-313974

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  14. LbCas12a-2C-NLS in pCSDest

    Plasmid
    #126638
    Purpose
    Expresses LbCas12a-2C-NLS in mammalian cell
    Depositor
    Scot Wolfe
    Publication
    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LbCas12a-2C-NLS
    Use
    CRISPR
    Tags
    3xHuman influenza hemagglutinin (HA)-Tag
    Expression
    Mammalian
    Promoter
    CMV IE94 promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126638-plasmid-map-sequence-id-247967

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  16. pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)

    Plasmid
    #47870
    Purpose
    All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.
    Depositor
    Erik Sontheimer, James Thomson
    Publication
    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    NmCas9
    U6pr-tracrRNA
    OCT4 crRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Available since
    Sept. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    47870-plasmid-map-sequence-id-68843

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  17. pUC57-Amp_CRISPIE donor B2

    Plasmid
    #172843
    Purpose
    CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPIE designer exon (phase 0-0) encoding mRuby3
    Use
    CRISPR
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172843-plasmid-map-sequence-id-340940

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  20. qTAG-N-Puro-moxGFP-LMNB1

    Plasmid
    #207773
    Purpose
    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207773-plasmid-map-sequence-id-413797

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Showing: 7321 - 7340 of 25284 results