We narrowed to 14,429 results for: nts

1. pcDNA3.2 mir-1-1 reporter mir16-1 wt

   Plasmid

   #46683

   Purpose

   Expresses wild-type miR-16-1 in mammalian cells

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   mir16-1 wt (MIR16-1 Human, Synthetic)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. E1b-GFP-Tol2-Gateway dre SE-zic2a S

   Plasmid

   #89388

   Purpose

   Vector for reporter assay of one zebrafish super-enhancer region

   Depositor

   Alena Shkumatava

   Article

   Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

   Insert

   zic2a (zic2a Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 15, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pmalc4x_E323K_TEV

   Plasmid

   #47699

   Purpose

   Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation E323K

   Depositor

   Raquel Lieberman

   Article

   Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

   Insert

   Maltose Binding Protein and Olfactomedin-like domain with TEV cleavage site (MYOC Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed glutamic acid 323 to lysine

   Promoter

   Ptac

   Available since

   Sept. 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pJG488

   Plasmid

   #91196

   Purpose

   WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   nTaCas9_H840A+gUbi8+gUbi1+donor
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-sgRNA_Dicer_3

   Plasmid

   #68809

   Purpose

   specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

   Depositor

   Constance Ciaudo

   Article

   Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

   Insert

   sgRNA mouse Dicer1
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. KHBD00120

   Plasmid

   #34274

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG3956 (sna Fly)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

7. FusionRed-Sit-N-15

   Plasmid

   #56133

   Purpose

   Localization: Golgi TGN, Excitation: 580, Emission: 608

   Depositor

   Michael Davidson

   Article

   Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

   Insert

   Sit (ST6GAL1 Human)
   

   Use

   Tags

   FusionRed

   Expression

   Mammalian

   Mutation

   aa 1-45 of SiT

   Promoter

   CMV

   Available since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. DCT.VV.Orf222

   Plasmid

   #12910

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf222
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

9. DCT.VV.Orf264

   Plasmid

   #12952

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf264
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

10. DCT.VV.Orf194

    Plasmid

    #12882

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf194
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

11. DCT.VV.Orf92

    Plasmid

    #12780

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf92
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

12. p35S::IRE1a

    Plasmid

    #135232

    Purpose

    constitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)

    Depositor

    Armin Djamei

    Article

    Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

    Insert

    IRE1a (IRE1A Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGHa-SERBP1(189-400)

    Plasmid

    #172315

    Purpose

    Expression of the C-terminal (189-400) domain of SERBP1 in E. coli.

    Depositor

    David Libich

    Article

    Baudin et al Biomol NMR Assign. 2021 Oct;15(2):461-46

    Insert

    Plasminogen Activator Inhibitor 1 RNA-binding protein, residues 189-400 (SERBP1 Human)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    deleted 1-188, 401-408

    Promoter

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pnEA-vH_His-TEV-DSep1

    Plasmid

    #174493

    Purpose

    bacterial expression of Drosophila Sep1

    Depositor

    Manos Mavrakis

    Article

    Mavrakis et al Nat Cell Biol. 2014 Apr;16(4):322-34. do

    Insert

    Sep1 (Sep1 Fly)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFAB1935

    Plasmid

    #47860

    Purpose

    BIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    his_T_min_linker_crp_T_min double terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    concatenate of his_min and crp_min

    Promoter

    pLTetO15

    Available since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAT9750-BEAR-mCherry

    Plasmid

    #162990

    Purpose

    BEAR target plasmid with split mCherry and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    mCherry split with an intron between amino acids 119-120
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAT15416-BEAR-mScarlet-target-BFP

    Plasmid

    #162996

    Purpose

    plasmid expressing an sgRNA targeting the BEAR-mScarlet plasmid along with a TagBFP marker

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    sgRNA targeting the BEAR-mScarlet plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

    Plasmid

    #160610

    Purpose

    Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    P35s:AsCas12a:T35s
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-sgRNA_Dicer_2

    Plasmid

    #68808

    Purpose

    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of

    Depositor

    Constance Ciaudo

    Article

    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

    Insert

    sgRNA mouse Dicer1
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAT-9222

    Plasmid

    #124222

    Purpose

    Cloning plasmid for EGxFP assay with self-targeting gRNA-s

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    self-targeting gRNA between EGFP halves
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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