We narrowed to 22,844 results for: Sis

1. CDS12_Cas9-NLS

   Plasmid

   #136122

   Purpose

   Puchta Arabidopsis codon-optimised Cas9 for CRISPR. CDS12 for N-term fusion with a CTAG

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CDS12_AtcoCas9-NLS(noStop)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBtPIPLC_N168C His

   Plasmid

   #173809

   Purpose

   Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

   Depositor

   Mary F Roberts, Anne Gershenson

   Article

   He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

   Insert

   Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Asn168Cys for labeling

   Promoter

   T7

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. HBT-CPK32ac-Flag

   Plasmid

   #107431

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK32 (CPK32 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Antares-SEPLuc C1A4E

   Plasmid

   #141088

   Purpose

   precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-IRESv24-SEP-C1A4E
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Please see depositor comments

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p5E-161K enhancer-NR2F2-MUT

   Plasmid

   #138371

   Purpose

   Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with NR2F2 binding sites mutated

   Depositor

   Nathan Lawson

   Article

   Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

   Insert

   161k human enhancer with conserved NR2F2 binding sites mutated
   

   Use

   Gateway compatible

   Tags

   Expression

   Mutation

   NR2F2 sites mutated

   Promoter

   none

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p5E-161K enhancer-ETS-CORE-MUT

   Plasmid

   #138372

   Purpose

   Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with ETS binding sites mutated

   Depositor

   Nathan Lawson

   Article

   Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

   Insert

   human 161K human enhancer with ETS bind sites mutated
   

   Use

   Gateway compatible

   Tags

   Expression

   Mutation

   ETS factor binding sites mutated

   Promoter

   NONE

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. hHSF4b K294R

   Plasmid

   #118357

   Purpose

   This plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hHSF4b (HSF4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K294R

   Promoter

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Gal4-HSF4b WT

   Plasmid

   #118358

   Purpose

   This plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hHSF4b (HSF4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHyg-AID(31-114)

   Plasmid

   #99514

   Purpose

   Truncated C-terminal AID degron cassette with HygR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(31-114) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   Expression

   Mutation

   internal inversion of nucleotides 265-312 of the …

   Promoter

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMCh-1474

    Plasmid

    #82420

    Purpose

    Plasmid for expression of HA-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 NED
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 1159-1698 from nvGW182

    Promoter

    CMV

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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