We narrowed to 14,429 results for: nts

1. pMOD_C2519

   Plasmid

   #91088

   Purpose

   Module C, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   OsU3

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. 625_HOXD13 in pmiRGlo

   Plasmid

   #78129

   Purpose

   luciferase reporter for miRNA activity

   Depositor

   Heidi Schwarzenbach

   Article

   Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

   Insert

   HOXD13 (HOXD13 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   3'UTR containing miRNA binding sites

   Promoter

   PGK

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. KHBD00638

   Plasmid

   #39705

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG3090 (Sox14 Fly)
   

   Use

   Gateway donr vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

4. KHBD00118

   Plasmid

   #34272

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG7345 (Sox21a Fly)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pCTCON2-BXG-2TAG

   Plasmid

   #158138

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   BFP-GFP fragment
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Cloned in a TAG codon at the first serine residue…

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. KHBD00732

   Plasmid

   #39749

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG3848 (trr Fly)
   

   Use

   Gateway donr vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

7. mKO-Fibrillarin-7

   Plasmid

   #57842

   Purpose

   Localization: Nuclear - Nucleolus, Excitation: 548, Emission: 561

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Fibrillarin (FBL Human)
   

   Use

   Tags

   mKO

   Expression

   Mammalian

   Mutation

   R36Q, S66F

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. CoV2-N-P13L

   Plasmid

   #177946

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P13L). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (P13L) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P13L

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CoV2-N-P80R

   Plasmid

   #177947

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (P80R) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P80R

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CoV2-N-S235F

    Plasmid

    #177956

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (S235F) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S235F

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. DCT.VV.Orf76

    Plasmid

    #12764

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf76
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)

    Plasmid

    #160635

    Purpose

    TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

    Insert

    AcrIIA4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO-sh-Myocardin

    Plasmid

    #100769

    Purpose

    Lentiviral expression of shRNA targeting MYOCD

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

    Insert

    Lenti-sh-Myocardin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. DCT.VV.Orf98

    Plasmid

    #12786

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf98
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pAT9753-BEAR-mScarlet-preedited

    Plasmid

    #162994

    Purpose

    BEAR control plasmid with split mScarlet and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    mScarlet split with an intron between amino acids 110-111
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. DCT.VV.Orf78

    Plasmid

    #12766

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf78
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pFAB3913

    Plasmid

    #47816

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD9 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 14 and BCD9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    see insert

    Available since

    Aug. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTH371-SUP45-N58A

    Plasmid

    #29378

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N58A mutant gene including genomic sequence 1432 …

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pTH397-SUP45-I222S

    Plasmid

    #29388

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    I222S mutant gene (corresponding to the sup45-2 a…

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pPK-351

    Plasmid

    #157921

    Purpose

    pcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBD

    Depositor

    Phillip Kyriakakis

    Article

    Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

    Inserts

    PIF3-MTAD
    IRES
    PhyB(1-621)-SV40NLS-Gal4DBD
    

    Use

    Synthetic Biology; Optogenetics

    Tags

    HA tag and SV40NLS

    Expression

    Mammalian

    Mutation

    pif3 1-523

    Promoter

    CMV

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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