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Plasmid#203795PurposeTol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Fzd1-mApple for zebrafish transgenesisDepositorInsertFzd1 (fzd1 Zebrafish)
UseZebrafish expressionTagsmAppleExpressionMutationPromoter4kb Wnt3 promoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pminiTol2-4kb wnt3 promoter-PMTmApple
Plasmid#203794PurposeTol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of PMTmApple for zebrafish transgenesisDepositorInsertPMT
UseZebrafish expressionTagsmAppleExpressionMutationPromoter4kb Wnt3 promoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-kappa-myc-dK-2xG4S-TMst
Plasmid#145766PurposeExpresses myc-dL5(E52K, L91S) low affinity Fluorogen Activating Protein (FAP) on the extracellular surface anchored to the mammalian cell plasma membrane with the PDGFR transmembrane domain.DepositorInsertkappa-myc-dK-2xG4S-TMst
UseTagsExpressionMammalianMutationThe dK FAP is dL5 with mutations at E50K, L89S, a…PromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS305-LEU2-pINO4-SV40NLS-PfV-GS-Sapphire
Plasmid#203655PurposeYeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.DepositorArticleInsertPfV
UseTagsSapphireExpressionYeastMutationPromoterAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-kappa-myc-dL5-2xG4S-TMst
Plasmid#73206PurposeExpresses myc-dL5(E52D)-TM (PDGFR derived) on the surface of mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)DepositorInsertkappa-myc-dL5-2XG4S-TM (MYC Human)
UseTagsThe FAP is fused with 2 copies of a G4S linker an…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-IgKappa-myc-dL5- 2XG4S-GPI
Plasmid#153308PurposeExpresses GPI-anchored dL5** in mammalian cells. (FAP-GPI)DepositorInsertIgKappa-myc-dL5-2XG4S-GPI
UseTagsmyc-dL5ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUBC-Pfv-Sapphire-IRES-H2B-mCherry
Plasmid#203648PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.DepositorArticleInsertPfV
UseLentiviralTagsSapphire-IRES-H2B-mCherryExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS403-HIS3-pINO4-SV40NLS-PfV-GS-Sapphire
Plasmid#203658PurposeYeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm GEMs under the HIS3 promoter.DepositorArticleInsertPfV
UseTagsSapphireExpressionYeastMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
iD20 LoxStopLox-Zim3-dCas9-mApple-hygro
Plasmid#204720PurposeCre-inducible CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3
Plasmid#221112PurposeFAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ERDepositorInsertMFA-IgKappa-FAPoptim-STE3
UseTagsExpressionYeastMutationPromoterSTE3Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only