Addgene

We narrowed to 16,288 results for: IGH@

Showing: 721 - 740 of 16288 results

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pAAV.CAG.Ruby2sm-Flag.WPRE.SV40

Plasmid

#98925

Purpose

AAV expression of Ruby2 spaghetti monster FLAG construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

Ruby2-smFP-Flag

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CBA

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-X1-Neo-RPN1-HA

Plasmid

#139868

Purpose

Lentiviral expression of HA-tagged (C terminal) human RPN1

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

RPN1 (Ribophorin1) (RPN1 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-UFM1-dCS

Plasmid

#139869

Purpose

Mammalian expression of HA-tagged human UFM1-cCS

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

UFM1 (UFM1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

d84-85

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pUB-spHRed

Plasmid

#84734

Purpose

Expresses pHRed in plant cells-biosensor for pHRed targeted to chloroplasts stoma

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

pHRed

Use

Tags

RbcS targeting sequence

Expression

Plant

Mutation

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-eKR2

Plasmid

#115337

Purpose

Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse

Depositor

Peter Hegemann

Article

Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.

Insert

eKR2

Use

Tags

EYFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-ReaChR-P2A-dTomato

Plasmid

#114368

Purpose

expresses a Cre-dependent red light-shifted channelrhodopsin variant

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

ReaChR-P2A-dTomato

Use

AAV

Tags

dTomato

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET28_Human_SAT1_Cterm_His

Plasmid

#220487

Purpose

heterologous expression of SSAT1 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Human_SAT1_Nterm_His

Plasmid

#220482

Purpose

heterologous expression of SSAT1 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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FCK-Mac-GFP

Plasmid

#22223

Purpose

Lentiviral expression of Mac-GFP for optogenetic inhibition

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Mac

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

NO

Promoter

CaMKII

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Arch-GFP

Plasmid

#37810

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Arch

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pUB-sSypHer (RbcS-SypHer)

Plasmid

#84732

Purpose

Expresses SypHer in plant cells-biosensor for pH targeted to chloroplasts stoma

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

sSypHer

Use

Tags

RbcS targeting sequence

Expression

Plant

Mutation

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pDS240

Plasmid

#34966

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Use

Tags

Expression

Yeast

Mutation

K533∆, high-affinity epitope

Promoter

GAL1

Available since

March 21, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-NDUFB2

Plasmid

#139841

Purpose

Lentiviral expression of human NDUFB2

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

NDUFB2 (NDUFB2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pT3TS-HiFi-nCas9n-A72

Plasmid

#125646

Purpose

micro-injection of poly-adenylated HiFi-nCas9n RNA

Depositor

Jason Berman

Article

Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

Insert

HiFi-nCas9n

Use

Rna in vitro transcription vector

Tags

Expression

Mutation

R691A from wild-type Cas9

Promoter

T3

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-hu FceRI beta

Plasmid

#8366

Depositor

Jean Kinet

Article

Kuster et al J Biol Chem 1992 Jun 25;267(18):12782-7.

Insert

human Fc epsilon RI beta cDNA (MS4A2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2005

Availability

Academic Institutions and Nonprofits only
iLID-mCherry-iTrkA

Plasmid

#136509

Purpose

Mammalian expression of iLID fused to mCherry and intracellular signaling domain of TrkA (aa 450-799)

Depositor

Bianxiao Cui

Article

Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

Insert

iLID mCherry iTrkA (Ntrk1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Mac-GFP

Plasmid

#58853

Purpose

AAV-mediated expression of Mac-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Mac-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Syn

Available since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Mac-GFP

Plasmid

#58849

Purpose

AAV expression of Mac-GFP under the CaMKII promoter

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Mac-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CaMKII

Available since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-X1-Neo-NDUFB4

Plasmid

#139840

Purpose

Lentiviral expression of human NDUFB4

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

NDUFB4 (NDUFB4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_SAT1 (human)

Plasmid

#220477

Purpose

heterologous expression of SSAT1 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Use

Tags

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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